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VinTS-tCRMod-GGSGGS7
Plasmid#111764PurposeExpresses vinculin tension sensor comprised of Clover-(GGSGGS)7-mRuby2 moduleDepositorInsertClover-mRuby2-based vinculin tension sensor with (GGSGGS)7 extensible domain
UseTagsExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDAS12342_PEAR-GFP_2in1
Plasmid#177184PurposePEAR-GFP plasmid with a pegRNA that targets its own plasmidDepositorInsertsEGFP split with an intron between amino acids 95-96
pegRNA targeting its own plasmid
UseCRISPRTagsExpressionMammalianMutationdisrupted 5' splice sitePromoterCMV and U6Available sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-RRBP1-2
Plasmid#92157PurposeCRISPR guide RNA targeting human RRBP1DepositorInsertRRBP1 sgRNA-2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
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SnFR-gamma8
Plasmid#165496PurposeGreen fluorescent glutamate reporter fused to TARP gamma-8 for mammalian expression.DepositorInsertiGluSnFR extracellular domain fused to gamma-8 via NETO2 TM domain (Cacng8 Rat, Synthetic)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-ChR2-TdTomato (Cre-OFF)
Plasmid#166610PurposeEncodes Cre-inactivated ChR2-TdTomato under control of the TREDepositorInsertChR2-TdTomato
UseAAVTagsExpressionMutationH134RPromotertetracycline response elementAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-mDLX-TVA-2A-N2cG
Plasmid#172364PurposeTargeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from inhibitory neuronsDepositorInsertTVA + B19-N2cG
UseAAVTagsExpressionMammalianMutationPromotermDLXAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Flp-DOG-NW
Plasmid#75469PurposeFlp recombinase dependent on GFP (Flp-DOG) for rAAV production and expression in mammalian cellsDepositorHas ServiceAAV1InsertFlp-DOG
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2CG2--3.5ubb:plin2-tdtomato
Plasmid#172534PurposeZebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with cmlc2:eGFP as a transgenesis markerDepositorInsert-3.5ubb:plin2-tdtomato
UseTagsExpressionMutationS80R, A81S, E144D, I148F and R415H mutations obse…Promoterzebrafish ubbAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCB
Plasmid#162699PurposeExpresses pHnCB10 derived carboxysome operon and prkDepositorInsertpHnCB10 derived carboxysome operon, prk
UseTagsExpressionBacterialMutationPromoterPLtet0-1 promoterAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFUChW Parkin shRNA Mul1 shRNA
Plasmid#78523PurposeLentiviral vector encoding two shRNAs and mCherry reporterDepositorUseLentiviral and RNAiTagsExpressionMutationPromoterH1Available sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Mito-CaNAR2
Plasmid#64731PurposeMitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of mitochondrial outer membrane.DepositorInsertCaNAR2
UseTagsCerulean3, N-terminal 30 amino acids from DAKAP1,…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-NanoLuc-15aa linker-βarrestin2-no HA-GS linker-TEVp
Plasmid#125235PurposeExpresses SPARK2 NanoLuc-βarrestin2-TEVp (no HA) in mammalian cellsDepositorInsertNanoLuc-15aa linker-βarrestin2-no HA-GS linker-TEVp
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-DIO-ReaChR-P2A-dTomato
Plasmid#114368Purposeexpresses a Cre-dependent red light-shifted channelrhodopsin variantDepositorInsertReaChR-P2A-dTomato
UseAAVTagsdTomatoExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-EF1-IRES-NEO mutant ITR
Plasmid#67905PurposePiggyBac transposon encoding Neomycin resistance with inverted terminal repeat mutationDepositorInsertNeoR (neoR Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-E2_Crimson
Plasmid#172377PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertE2_Crimson
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-rTH-tTA
Plasmid#133268PurposeAn AAV genome that expresses tTA from the rat TH promoterDepositorInserttTA
UseAAVTagsExpressionMammalianMutation*(see below)PromoterrTHAvailable sinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYP-mRuby3
Plasmid#179727PurposeLentiviral expression of SYP-mRuby3 for use as a synaptic vesicle markerDepositorInsertSYP (Syp Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDest-303
Plasmid#159678PurposeGateway destination vector for mammalian protein expressionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only
