We narrowed to 4,583 results for: bre
-
Plasmid#211893PurposeVinculin tension sensor (VinTS) with vinculin S1033 phosphomimetic point mutation (S1033D), in lentiviral expression vector.DepositorInsertVinculinTS-S1033D (VCL Chicken, Synthetic)
UseLentiviralMutationmutated vinculin serine 1033 to aspartic acid (S1…PromoterCMVAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRRL-VinculinCS-S1033A
Plasmid#211894PurposeVinculin conformation sensor (VinCS) with vinculin S1033 unphosphorylatable point mutation (S1033A), in lentiviral expression vector.DepositorInsertVinculinCS-S1033A (VCL Chicken, Synthetic)
UseLentiviralMutationmutated vinculin serine 1033 to alanine (S1033A),…PromoterCMVAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRRL-VinculinCS-S1033D
Plasmid#211895PurposeVinculin conformation sensor (VinCS) with vinculin S1033 phosphomimetic point mutation (S1033D), in lentiviral expression vector.DepositorInsertVinculinCS-S1033D (VCL Chicken, Synthetic)
UseLentiviralMutationmutated vinculin serine 1033 to aspartic acid (S1…PromoterCMVAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHW491
Plasmid#198808PurposeIn vivo blocking of synaptic signaling through Tetanus toxin mediated cleavage of synptobrevin proteins.DepositorInsert15xUAS::Tetx cDNA::let-858 3'UTR
ExpressionWormAvailable SinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pROS13_IDH2 #7
Plasmid#166102PurposeThis plasmid express a sgRNA that targets the IDH2 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH2-135 by homologous recombination.DepositorArticleAvailable SinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pROS13_IDH1 #4
Plasmid#166101PurposeThis plasmid express a sgRNA that targets the IDH1 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH1-67 by homologous recombination.DepositorArticleAvailable SinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
SNX17-PX (1-111)
Plasmid#119098PurposeBacterial expression of human phox homology (PX) domain, SNX17-PX (1-111)DepositorAvailable SinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
SNX16-PX (104-216)
Plasmid#119097PurposeBacterial expression of human phox homology (PX) domain, SNX16-PX (104-216)DepositorInsertSNX16-PX (104-216) (SNX16 Human)
PromoterN/AAvailable SinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
SNX4-PX (48-186)
Plasmid#119085PurposeBacterial expression of human phox homology (PX) domain, SNX4-PX (48-186)DepositorAvailable SinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
SNX32-PX (17-166)
Plasmid#119111PurposeBacterial expression of human phox homology (PX) domain, SNX32-PX (17-166)DepositorAvailable SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
SNX7-PX (89-218)
Plasmid#119088PurposeBacterial expression of human phox homology (PX) domain, SNX7-PX (89-218)DepositorAvailable SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
SNX1-PX (142-269)
Plasmid#119082PurposeBacterial expression of human phox homology (PX) domain, SNX1-PX (142-269)DepositorAvailabilityAcademic Institutions and Nonprofits only -
SNX2-PX (136-279)
Plasmid#119083PurposeBacterial expression of human phox homology (PX) domain, SNX2-PX (136-279)DepositorAvailabilityAcademic Institutions and Nonprofits only -
SNX8-PX (70-190)
Plasmid#119089PurposeBacterial expression of human phox homology (PX) domain, SNX8-PX (70-190)DepositorAvailabilityAcademic Institutions and Nonprofits only -
SNX15-PX (1-127)
Plasmid#119096PurposeBacterial expression of human phox homology (PX) domain, SNX15-PX (1-127)DepositorAvailabilityAcademic Institutions and Nonprofits only -
SNX23-PX (1178-1312)
Plasmid#119102PurposeBacterial expression of human phox homology (PX) domain, SNX23-PX (1178-1312)DepositorInsertSNX23-PX (1178-1312)
TagsGSTExpressionBacterialPromotertacAvailabilityAcademic Institutions and Nonprofits only -
SNX24-PX (1-101)
Plasmid#119103PurposeBacterial expression of human phox homology (PX) domain, SNX24-PX (1-101)DepositorAvailabilityAcademic Institutions and Nonprofits only -
PXK-PX (1-125)
Plasmid#119114PurposeBacterial expression of human phox homology (PX) domain, PXK-PX (1-125)DepositorAvailabilityAcademic Institutions and Nonprofits only -
PI3KC2beta-PX (1348-1487)
Plasmid#119120PurposeBacterial expression of human phox homology (PX) domain, PI3KC2beta-PX (1348-1487)DepositorAvailabilityAcademic Institutions and Nonprofits only -
RICS-PX (127-255)
Plasmid#119126PurposeBacterial expression of human phox homology (PX) domain, RICS-PX (127-255)DepositorInsertRICS-PX (127-255)
TagsGSTExpressionBacterialPromotertacAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-HA-CAD
Plasmid#46238PurposeFor expression of HA and FLAG-tagged murine CAD in mammalian cellsDepositorInsertcarbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)
TagsFLAG and HAExpressionMammalianPromoterCMVAvailable SinceNov. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA Myc DBC1
Plasmid#35096DepositorAvailable SinceJuly 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-oA ONE-GO
Plasmid#189732PurposeGPCR/G protein BRET biosensorDepositorAvailable SinceJan. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTol2CG2-hsp70I:QFGal4-SV40pA
Plasmid#155120PurposeTol2 vector for heat-shock inducible expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis markerDepositorInserthsp70l:QFGal4 (hsp70l Budding Yeast, Neurospora crassa, Zebrafish)
UseZebrafish expressionPromoterhsp70lAvailable SinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
hGFAP-GFP
Plasmid#40592DepositorInsertGFP-S65T
UseMouse TargetingTagsMP-1 fragment (mouse protamine 1 intron and polya…ExpressionBacterial and MammalianMutationConstruct contains a mutated, humanized hGFP(S65T…PromoterhGFAP promoterAvailable SinceOct. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
VAMP3-mCherry
Plasmid#92423PurposeExpression of VAMP3 C-terminally conjugated to mCherry.DepositorAvailable SinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMax_ScaI_BFP
Plasmid#177824PurposeSite-specific mutagenesis of tagBFP for the purposes of creating a clean double strand break for the purposes of measuring NHEJ activity via Host Cell Reactivation (HCR).DepositorInserttagBFP
ExpressionMammalianMutation5' inserted ScaI RE cut sitePromoterCMVAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-13 ONE-GO
Plasmid#189740PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralMutationL342M (present in YFP but no functional consequen…Available SinceJan. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLVX-CMV-Galpha-12 ONE-GO
Plasmid#189739PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralAvailable SinceJan. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLVX-CMV-Galpha-z ONE-GO
Plasmid#189734PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralAvailable SinceJan. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLVX-CMV-Galpha-oB ONE-GO
Plasmid#189733PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralAvailable SinceJan. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEM-Cas9HF1-recA56
Plasmid#89962PurposeModified from pEM-Cas9HF1 (Addgene ID: 89961) to include constitutive recA56 to block recA-mediated double-strand break repair.DepositorInsertsCas9HF1
recA56
ExpressionBacterialMutationN497A/R661A/Q695A/Q926APromoterpTet and proDAvailable SinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
Rlucrarr3-Flash5
Plasmid#74133PurposeFlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 263 of Arrestin.DepositorInsertArrestin3
TagsRenilla luciferase (rLuc)ExpressionMammalianMutationInsertion of FlAsH motif (CCPGCC) following amino…Available SinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Gluc-LHCGR
Plasmid#221781PurposeMeasurement of glycoprotein-glycoprotein receptor binding using the BRET phenomenon and measurement of membrane expression of the Gluc-LHCGRDepositorAvailable SinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
BK alpha subunit
Plasmid#113566Purposemouse BK channel alpha subunit expression vectorDepositorAvailable SinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
Rlucrarr3-Flash6
Plasmid#74134PurposeFlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 410 of Arrestin3.DepositorInsertArrestin3
TagsRenilla luciferase (rLuc)ExpressionMammalianMutationInsertion of FlAsH motif (CCPGCC) following amino…Available SinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Rlucrarr3-Flash1
Plasmid#74129PurposeFlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 40 of Arrestin3.DepositorInsertArrestin3
TagsRenilla luciferase (rLuc)ExpressionMammalianMutationInsertion of FlAsH motif (CCPGCC) following amino…Available SinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
Rlucrarr3-Flash4
Plasmid#74132PurposeFlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 225 of Arrestin3.DepositorInsertArrestin3
TagsRenilla luciferase (rLuc)ExpressionMammalianMutationInsertion of FlAsH motif (CCPGCC) following amino…Available SinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Rlucrarr3-Flash3
Plasmid#74131PurposeFlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 171 of Arrestin3.DepositorInsertArrestin3
TagsRenilla luciferase (rLuc)ExpressionMammalianMutationInsertion of FlAsH motif (CCPGCC) following amino…Available SinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Rlucrarr3-Flash2
Plasmid#74130PurposeFlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 139 of Arrestin3.DepositorInsertArrestin3
TagsRenilla luciferase (rLuc)ExpressionMammalianMutationInsertion of FlAsH motif (CCPGCC) following amino…Available SinceApril 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEM-Cas9HF1-indRecA56
Plasmid#102294PurposeModified from pEM-Cas9HF1 (Addgene ID: 89961) to co-express inducible recA56 to block recA-mediated double-strand break repair.DepositorInsertsCas9HF1
recA56
UseCRISPRExpressionBacterialMutationN497A/R661A/Q695A/Q926APromoterpTetAvailable SinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmiCas9-msa
Plasmid#164833PurposemiCas9-msa fusion protein expressing plasmidDepositorInsertmiCas9-msa
UseCRISPRTagsFLAG and NLSExpressionMammalianMutationcontains mSA sequence range is 5106-5447 and Brex…PromoterCMVAvailable SinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-hSyn-Galpha-13 ONE-GO
Plasmid#204337PurposeGPCR/G protein BRET biosensorDepositorInserthSyn Galpha-13 ONE-GO (GNA13 Human)
UseLentiviralAvailable SinceJan. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTol2CG2-acta1:QFGal4-SV40pA
Plasmid#155121PurposeTol2 vector for skeletal-muscle expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis markerDepositorInsertacta1:QFGal4
UseZebrafish expressionPromoteracta1Available SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEND-304_Cacnes_tetR
Plasmid#225619PurposeC. acnes with tetR-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+TetR // P(BBa_23119-TetO1O3)+RBS_1+mCherryDepositorInsertTetR
UseSynthetic BiologyTags6xHis and mCherry-V5-6xHisExpressionBacterialMutationCodon optimized for Cutibacterium acnesAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEND-375_Cacnes_phlF
Plasmid#225620PurposeC. acnes with PhlF-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+PhlF // P(BBa_23119-PhlO1O3)+RBS_1+mCherry.DepositorInsertPhlF
UseSynthetic BiologyTags6xHis and mCherry-V5-6xHisExpressionBacterialMutationCodon optimized for Cutibacterium acnesAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-PSMB5-PSMB6-PSMB7-TEV-2xSTII
Plasmid#217896PurposeInsect cell expression of human 20S CP proteasome subunit beta type-5 (PSMB5), beta type-6 (PSMB6), and beta type-7 (PSMB7) with C-terminal Twin-Strep-tagDepositorAvailable SinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-PSMA5-PSMA6-PSMA7
Plasmid#217894PurposeInsect cell expression of human 20S CP proteasome subunit alpha type-5 (PSMA5), alpha type-6 (PSMA6), and alpha type-7 (PSMA7)DepositorAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
SNX25-PX (506-628)
Plasmid#119104PurposeBacterial expression of human phox homology (PX) domain, SNX25-PX (506-628)DepositorAvailable SinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
SNX14-PX (561-686)
Plasmid#119095PurposeBacterial expression of human phox homology (PX) domain, SNX14-PX (561-686)DepositorAvailable SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only