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pcDNA3.1 3xF-GAR1

Plasmid

#126873

Purpose

Expresses 3xFlag GAR1

Depositor

Kathleen Collins

Publication

Egan et al Mol Cell Biol. 2010 Jun;30(11):2775-86.

Insert

GAR1 (GAR1 Human)

Tags

3xFlag

Expression

Mammalian

Available since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pVEGFR2_TEV(N)_NES_TCS(Q'L)_HA-dCas9(N)_P2A_Puro-WPRE

Plasmid

#101104

Purpose

Encodes dCas9(N) fused to VEGFR2

Depositor

Tudor Fulga

Publication

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

VEGFR2-dCas9(N)

Use

CRISPR and Synthetic Biology

Tags

HA

Expression

Mammalian

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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M4755 KanMX::LEU2 Disruptor Converter

Plasmid

#51685

Purpose

Yeast marker swap plasmid for converting KanMX3 to LEU2

Depositor

David Stillman

Publication

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

LEU2 (LEU2 Budding Yeast)

Use

Yeast marker swap

Expression

Yeast

Available since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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pBR322 14-3-3 Promoter

Plasmid

#16517

Depositor

Bert Vogelstein

Publication

Hermeking et al Mol Cell. 1997 Dec . 1(1):3-11.

Insert

14-3-3 promoter (SFN Human)

Expression

Bacterial

Available since

July 27, 2008

Availability

Academic Institutions and Nonprofits only
pAc5.1B-EGFP-DmDCP1

Plasmid

#21684

Depositor

Elisa Izaurralde

Publication

Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

Insert

Decapping protein 1 (Dcp1 Fly)

Use

Drosophila expression

Tags

EGFP

Available since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-NM

Plasmid

#1127

Depositor

Susan Lindquist

Publication

Scheibel et al Nat Struct Biol 2001 Nov;8(11):958-62.

Insert

NM (SUP35 Budding Yeast)

Tags

GST

Expression

Bacterial

Available since

March 14, 2006

Availability

Academic Institutions and Nonprofits only
VCA

Plasmid

#27792

Depositor

Steven Vogel

Publication

Koushik et al PLoS One. 2009 Nov 25;4(11):e8031. doi:

Insert

Venus-Cerulean-Amber

Use

Stoichiometry standard

Expression

Mammalian

Available since

June 23, 2011

Availability

Academic Institutions and Nonprofits only
AgAK

Plasmid

#71290

Purpose

Expression of A. gambiae adenosine kinase

Depositor

Vern Schramm

Publication

Cassera et al Biochemistry. 2011 Mar 22;50(11):1885-93

Insert

Adenosine Kinase

Tags

6X Histidine

Expression

Bacterial

Promoter

T7

Available since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only
pGL4 mIL-17 1.1kb promoter

Plasmid

#20125

Depositor

Warren Strober

Publication

Fuping et al 2008 Nov;9(11):1297-306. Epub 2008 Oct 1

Insert

mIL-17 (Il17a Mouse)

Use

Luciferase

Available since

Jan. 16, 2009

Availability

Academic Institutions and Nonprofits only
BDS-2 3x MUT (p53 binding site)

Plasmid

#16516

Depositor

Bert Vogelstein

Publication

Hermeking et al Mol Cell. 1997 Dec . 1(1):3-11.

Insert

p53 binding site (SFN Human)

Tags

Luciferase

Expression

Mammalian

Mutation

Mutant p53 binding sites

Available since

March 28, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
3xFlag-HaloTag-SUZ12 HDR - pDY082

Plasmid

#171110

Purpose

Homology directed repair plasmid for generating HaloTag-SUZ12 cells

Depositor

Thomas Cech

Publication

Youmans et al Genes Dev. 2018 Jun 1;32(11-12):794-805.

Insert

SUZ12 (SUZ12 Human)

Tags

HaloTag

Expression

Mammalian

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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3xFlag-HaloTag-EZH2 HDR - pDY053

Plasmid

#171108

Purpose

Homology directed repair plasmid for generating HaloTag-EZH2 cells

Depositor

Thomas Cech

Publication

Youmans et al Genes Dev. 2018 Jun 1;32(11-12):794-805.

Insert

EZH2 (EZH2 Human)

Tags

HaloTag

Expression

Mammalian

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLV-mNeon-TurboID

Plasmid

#186610

Purpose

Third generation lentiviral vector expressing mNeonGreen fused to V5-TurboID

Depositor

Gia Voeltz

Publication

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

mNeon-V5-TurboID

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Mutation

For reference see Branon et al., 2018

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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EasyFusion mCherry

Plasmid

#112849

Purpose

General donor vector with mCherry coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Publication

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

mCherry

Use

Synthetic Biology

Promoter

No

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-rat Nrxn2beta AS4(-)

Plasmid

#58268

Purpose

Expression plasmid for HA-tagged rat Neurexin 2beta, lacking the insert at alternatively spliced segment 4 (AS4-)

Depositor

Peter Scheiffele

Publication

Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

Insert

Nrxn2beta AS4(-) (Nrxn2 Rat)

Tags

HA

Expression

Mammalian

Promoter

CAG

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pET-UbcH10-DN

Plasmid

#8502

Depositor

Joan Ruderman

Publication

Bastians et al Mol Biol Cell 1999 Nov;10(11):3927-41.

Insert

UbcH10 (UBE2C Human)

Tags

His

Expression

Bacterial

Mutation

Dominant Negative, C114S

Available since

June 1, 2005

Availability

Academic Institutions and Nonprofits only
pDDGFP2_leu2d_NRT1.1

Plasmid

#58332

Purpose

Expresses Arabidopsis thaliana NRT1.1 in Saccharomyces cells as a C-terminal GFP his tagged protein

Depositor

Simon Newstead

Publication

Parker et al Nature. 2014 Mar 6;507(7490):68-72. doi:

Insert

NRT1.1 (NRT1.1 Mustard Weed)

Tags

GFP-His

Expression

Yeast

Promoter

GAL1

Available since

Aug. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pLX307/PTPN11 E76G

Plasmid

#140862

Purpose

expresses PTPN11 E76G

Depositor

Kimberly Stegmaier

Publication

Cremer et al Cancer Discov. 2020 Feb;10(2):214-231. d

Insert

PTPN11 protein tyrosine phosphatase non-receptor type 11 [ Homo sapiens (human) ] (PTPN11 Human)

Expression

Mammalian

Mutation

E76G

Promoter

EF1a

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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TAF3 PHD_pGEX-6P-2

Plasmid

#92100

Purpose

Expresses GST fusion TAF3 PHD domain protein

Depositor

Albert Jeltsch

Publication

Kungulovski et al Epigenetics Chromatin. 2016 Mar 22;9:11.

Insert

TAF3 PHD domain (TAF3 Human)

Tags

GST Tag

Expression

Bacterial

Promoter

tac promoter

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA GAL4 CREB 1-341

Plasmid

#22972

Depositor

Marc Montminy

Publication

Conkright et al Mol Cell. 2003 Apr . 11(4):1101-8.

Insert

CREB

Tags

GAL4 DNA-binding domain

Expression

Mammalian

Available since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only