164,909 results

1. MSCV-opEBNA2-IRES-mCherry

   Plasmid

   #220355

   Purpose

   Expresses EBV latent gene EBNA2 in mammalian cells (EBNA2 coding sequence was optimised for expression)

   Depositor

   Klaus Rajewsky

   Article

   Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

   Insert

   opEBNA2 (EBNA-2 Epstein-Barr Virus (EBV) strain B95-8)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   codon-optimised for expression in mammalian cells

   Promoter

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBbA6c-RFP

   Plasmid

   #35290

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   LlacO-1

   Available since

   Feb. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNP566

   Plasmid

   #222724

   Purpose

   pNP561 - IS621 WT Target with T7 Inducible IS621 Recombinase

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

   Insert

   IS621 Recombinase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOsTIR1w/oGFP

   Plasmid

   #102883

   Purpose

   Expresses OsTIR1 via the ADH1 promoter in yeast. The cassette is integrated in the HO locus. Assembly and characterization of the auxin-inducible degradation.

   Depositor

   Matthias Heinemann

   Article

   Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

   Insert

   OsTIR1
   

   Use

   Tags

   3x myc

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-PB1-B/BRISBANE/60/2008

   Plasmid

   #187919

   Purpose

   Expresses PB1 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

   Depositor

   Arindam Mondal

   Article

   Kedia et al Front Microbiol. 2022 May 25;13:868367.

   Insert

   polymerase basic protein 1
   

   Use

   Tags

   UNTAGGED

   Expression

   Mutation

   Promoter

   CMV Promoter

   Available since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR_SFFv_4D5-WT-Highest-CAR_RHL004

   Plasmid

   #164826

   Purpose

   Lentiviral vector for constitutive expression of AntiHER2 4D5-WT-Highest CAR

   Depositor

   Wendell Lim

   Article

   Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

   Insert

   AntiHER2 4D5-WT Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. TurboID-Cyclin D1

   Plasmid

   #215084

   Purpose

   Expresses TurboID-Cyclin D1 in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Retroviral

   Tags

   TurboID

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

   Plasmid

   #139987

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTF-lacZ-rfp

   Plasmid

   #153037

   Purpose

   CRISPR-Cas12a genome editing in E. coli. Contains a constitutively CRISPR array targeting the lacZ gene in E. coli genome and donor DNA to insert an constitutively expressed rfp reporter gene.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

   Insert

   CRISPR array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAG423GPD-ccdB

    Plasmid

    #14150

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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