169,273 results

1. pDB5321

   Plasmid

   #204831

   Purpose

   An integrating plasmid containing both the inducible enotetSW3 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.

   Depositor

   Li-Lin Du

   Article

   Lyu et al J Cell Sci. 2024 Nov 1;137(21):jcs263404

   Inserts

   tetR
   mECitrine
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   CMV promoter and enotetSW3 promoter

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDonor14-N7HELIX-KanR

   Plasmid

   #190663

   Purpose

   Kanamycin resistance donor for N7HELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   KanR, R6K origin of replication
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   NA

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. MLRV2: NF-kb-SMAD-DBE-P53-AP1

   Plasmid

   #124536

   Purpose

   Multiplex luciferase reporter vector, with luciferase reporters for the pathways NFKB, SMAD, FOXO, P53 and AP1

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

   Inserts

   5 copies of the NF-kb DNA binding motif
   7xSMAD_RE::FLuc
   3xDBE_BE::Renilla (FOXO)
   2xP53_RE::NLuc
   6xAP-1_RE::GrRenilla
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-EF1a-fDIO-Cre (AAV8)

   Viral Prep

   #121675-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-EF1a-fDIO-Cre (#121675). In addition to the viral particles, you will also receive purified pAAV-EF1a-fDIO-Cre plasmid DNA. EF1a-driven, Flp recombinase dependent expression of Cre recombinase. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Esteban Engel, Alexander Nectow

   Article

   Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30

   Promoter

   EF1a

   Tags

   None

   Available since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

5. pEGFP-hsRPA14 (MSW#503)

   Plasmid

   #208069

   Purpose

   Expression of GFP-tagged hsRPA3 in human cells

   Depositor

   Marc Wold

   Article

   Haring et al J Biol Chem. 2008 Jul 4;283(27):19095-11

   Insert

   RPA3 (RPA3 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. HA Raptor

   Plasmid

   #8513

   Depositor

   David Sabatini

   Article

   Kim et al Cell 2002 Jul 26;110(2):163-75.

   Insert

   Raptor (RPTOR Human)
   

   Use

   Tags

   ha

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. HA Raptor

   Plasmid

   #8513

   Depositor

   David Sabatini

   Article

   Kim et al Cell 2002 Jul 26;110(2):163-75.

   Insert

   Raptor (RPTOR Human)
   

   Use

   Tags

   ha

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmiRFP670-N1

   Plasmid

   #79987

   Purpose

   Monomeric near-infrared fluorescent protein miRFP670 in mammalian plasmid

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

   Has Service

   Cloning Grade DNA

   Insert

   miRFP670
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-Flag-H3.3-polyA

   Plasmid

   #59780

   Purpose

   Express Flag-tagged H3.3 (H3F3B) in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   H3F3B (H3-3B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Sept. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCE059-SiT-Cas12a-[Activ]

    Plasmid

    #128136

    Purpose

    Express both AsCas12a fused to [Activ] activator domain and CRISPR arrays on a single transcript

    Depositor

    Randall Platt

    Article

    Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

    Insert

    AsCas12a-[Activ]-Triplex
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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