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phABCA3-eGFP_CRISPR_Donor

Plasmid

#188540

Purpose

Homologous recombination donor plasmid for CRISPR/Cas9 targeting of GFP fusion protein to the stop codon of endogenous human ABCA3 gene locus

Depositor

Darrell Kotton

Article

Sun et al Am J Respir Cell Mol Biol. 2021 Oct;65(4

Insert

EGFP

Use

CRISPR; Donor for homologous recombination

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLC-Flag-SENP8 sg2R-P2A-Hygro

Plasmid

#124300

Purpose

Lentiviral vector for expression of Flag tagged SENP8-P2A-Hygro casette from a CMV promoter. SENP8 cDNA contains silent mutations to disrupt a sgRNA binding site.

Depositor

Eva Gottwein

Article

Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

Insert

SENP8 (SENP8 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

several silent mutations to disrupt sgRNA targeti…

Promoter

CMV

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pEMS2046

Plasmid

#79654

Purpose

ssAAV genome with Ple257 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

ssAAV Ple257-EmGFP WPRE

Use

AAV

Promoter

PAX6 Enhancer

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pEMS1930

Plasmid

#79779

Purpose

High copy number pUC vector containing Ple257 (PAX6 MiniPromoter) insert

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

Ple257

Use

Pleiades promoter project [sic, pleaides plieades]

Promoter

Ple257 (PAX6 MiniPromoter)

Available Since

Nov. 15, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2047

Plasmid

#79655

Purpose

ssAAV genome with Ple258 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

ssAAV Ple258-EmGFP WPRE

Use

AAV

Promoter

PAX6 Enhancer

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pEMS1931

Plasmid

#79780

Purpose

High copy number pUC vector containing Ple258 (PAX6 MiniPromoter) insert

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

Ple258

Use

Pleiades promoter project [sic, pleaides plieades]

Promoter

Ple258 (PAX6 MiniPromoter)

Available Since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1929

Plasmid

#79778

Purpose

High copy number pUC vector containing Ple256 (PAX6 MiniPromoter) insert

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

Ple256

Use

Pleiades promoter project [sic, pleaides plieades]

Promoter

Ple256 (PAX6 MiniPromoter)

Available Since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1928

Plasmid

#79777

Purpose

High copy number pUC vector containing Ple255 (PAX6 MiniPromoter) insert

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

Ple255

Use

Pleiades promoter project [sic, pleaides plieades]

Promoter

Ple255 (PAX6 MiniPromoter)

Available Since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1927

Plasmid

#79776

Purpose

High copy number pUC vector containing Ple254 (PAX6 MiniPromoter) insert

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

Ple254

Use

Pleiades promoter project [sic, pleaides plieades]

Promoter

Ple254 (PAX6 MiniPromoter)

Available Since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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213-petDuet1-GBD-LUMP--cdc42

Plasmid

#65973

Purpose

Dual expression of GBD-LUMP and cdc42

Depositor

Gerard Marriott

Article

Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. p

Inserts

LUMP
cell division cycle 42 (CDC42 Human)

Tags

GBD (GTPase binding domain from ACK1; residues 44…

Expression

Bacterial

Mutation

constitutively active Cdc42 (Q61L)

Promoter

T7

Available Since

July 13, 2015

Availability

Academic Institutions and Nonprofits only

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LCV2 IRF3 KO

Plasmid

#217446

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human IRF3

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Insert

sgRNA targeting IRF3 (IRF3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-eGFP-53BP1

Plasmid

#60813

Purpose

mammalian expression vector

Depositor

Daniel Durocher

Article

Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

Insert

53BP1 (TP53BP1 Human)

Tags

eGFP

Expression

Mammalian

Available Since

Dec. 9, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pHAGE-FGFR3__TACC3

Plasmid

#116818

Purpose

Lentiviral expression of FGFR3__TACC3 fusion

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

FGFR3__TACC3

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pVSV-G

Plasmid

#138479

Purpose

Expresses VSV-G envelope protein for pseudotyping NanoMEDIC particle.

Depositor

Akitsu Hotta

Article

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

VSV-G envelop protein

Expression

Mammalian

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pSLQ5428_pHR_EF1a-mCherry-P2A-Rfx_Cas13d-2xNLS-3xFLAG

Plasmid

#155305

Purpose

Lentiviral vector encoding Rfx Cas13d fused with 2xNLS, 3xFLAG, and 2A-tagged mCherry.

Depositor

Stanley Qi

Article

Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

Insert

mCherry 2A-tagged to Ruminococcus flavefaciens XPD3002 Cas13d

Use

Lentiviral

Tags

2xNLS-3xFLAG

Expression

Mammalian

Promoter

EF1a

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-ACTA2_gRNA1-SpCas9-T2A-GFP

Plasmid

#126706

Purpose

to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human ACTA2 locus at the end of the endogenous coding sequence

Depositor

Darrell Kotton

Article

Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

Insert

ACTA2_gRNA1-SpCas9-T2A-GF (ACTA2 Human)

Use

CRISPR

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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psd44-iGFP-MFGE8-long

Plasmid

#46847

Purpose

expression of MFGE8 in mammalian cells

Depositor

Agnese Mariotti

Article

Carrascosa et al Oncogene. 2012 Mar 22;31(12):1521-32. do

Insert

MFGE8 (Mfge8 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK promoter

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only
pHAGE-EF1aL-WT_ABCA3-GFP

Plasmid

#188548

Purpose

Lentiviral vector allowing for EF1aL-driven constitutive expression of wildtype human ABCA3:GFP fusion protein

Depositor

Darrell Kotton

Article

Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

Insert

ABCA3:GFP (ABCA3 Human)

Use

Lentiviral

Tags

GFP

Promoter

EF1aL

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pFB2D

Plasmid

#195286

Purpose

Bicistronic DTR-EGFP fusion and Puromycin resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

DTR EGFP 2A Puro

Expression

Mammalian

Promoter

EFS

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pFB2E

Plasmid

#195288

Purpose

Bicistronic HSV-TK-EGFP fusion and Puromycin resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

HSV-TK EGFP 2A Puro

Expression

Mammalian

Promoter

EFS

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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