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EF1a_MYOD1_P2A_Hygro_Barcode

Plasmid

#120464

Purpose

Barcoded lentiviral vector to express MYOD1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MYOD1 (MYOD1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-mRuby2

Plasmid

#99117

Purpose

An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein mRuby2

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

DIO-mRuby2

Use

AAV

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-mTurquoise2

Plasmid

#99115

Purpose

An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein mTurquoise2

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

DIO-mTurquoise2

Use

AAV

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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delCMV-mCherry-3x-p67phox-GBD

Plasmid

#214141

Purpose

Rac activity sensor

Depositor

Leif Dehmelt

Article

Nanda et al Nat Commun. 2023 Dec 15;14(1):8356. doi:

Insert

3xp67phox-GBD (NCF2 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Only the GTPase binding domain (GBD) is included

Promoter

delCMV

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-Galpha-i3-IRESWT-Gbetagamma ONE-GO

Plasmid

#204341

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

Galpha-i3/Gbetagamma ONE-GO

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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mCherry-C1-TGAT

Plasmid

#84334

Purpose

Expresses TGAT tagged with mCherry

Depositor

Dorus Gadella

Article

van Unen et al BMC Cell Biol. 2018 Jun 7;19(1):6. doi:

Insert

TGAT (TRIO Human)

Tags

mCherry

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Dec. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-miRFP720-HO1

Plasmid

#197257

Purpose

Expresses the protein of miRFP720 and HO1 in bacteria

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP720

Expression

Bacterial

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-miRFP680-HO1

Plasmid

#197253

Purpose

Expresses the protein of miRFP680 and HO1 in bacteria

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP680

Expression

Bacterial

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pTubulin-miRFP680

Plasmid

#197239

Purpose

Expresses the fusion protein of Tubulin-miRFP680 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

Tubulin-miRFP680

Expression

Mammalian

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCWU6-xylR-E-lepB

Plasmid

#190725

Purpose

fine tune expression of type 1 signal peptidase under the control of xylose- and theophylline- inducible system

Depositor

Chenggang Wu

Article

Bibek et al bioRxiv. 2023 Apr 24:2023.04.24.538132.

Insert

xylR-E-lepB

Expression

Bacterial

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-BREP

Plasmid

#172337

Purpose

BRET-Based Bioluminescent Red Protein

Depositor

Huiwang Ai

Article

Tian et al Nat Commun. 2022 Jul 8;13(1):3967. doi:

Insert

BREP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pQZ083

Plasmid

#106417

Purpose

Express Complexin-1 fragment (1-83) in E. coli

Depositor

Axel Brunger

Article

Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d

Insert

complexin-1 fragment 1-83 (Cplx1 Rat, Synthetic)

Tags

GST tag

Expression

Bacterial

Mutation

Fragment 1-83, Codon optimization

Promoter

T7

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pDC183

Plasmid

#106415

Purpose

Co-expresses trans SNARE complex with pQZ210 in E. coli

Depositor

Axel Brunger

Article

Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d

Inserts

SNAP25 fragment 141-204 (Snap25 Rat, Synthetic)
SNAP25 Fragment 7-83 (Snap25 Rat, Synthetic)

Expression

Bacterial

Mutation

Fragment 141-204, Codon optimization and Fragment…

Promoter

T7

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pET21a-mAIM2-GST

Plasmid

#51533

Purpose

Express GST-tagged mAIM2 in bacteria

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

Aim2 (Aim2 Mouse)

Tags

GST

Expression

Bacterial

Promoter

lac operator

Available Since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

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pET21a-hAIM2-GST

Plasmid

#51528

Purpose

Express GST-tagged hAIM2 in bacteria

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Tags

GST

Expression

Bacterial

Promoter

lac operator

Available Since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

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(133) pcDNA3.1-Nup62-Flag-EPEA

Plasmid

#160744

Purpose

Expresses the nucleoporin Nup62 with C-terminal Flag and EPEA tags. Proteins of interest can be inserted by replacing Nup62 using Sgfl + Sgsl restriction enzymes

Depositor

Christina Woo

Article

Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

Insert

Nup62-Flag-EPEA

Tags

EPEA and Flag

Expression

Mammalian

Promoter

T7/CMV

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ150-GFP(dpiRNA)::CDK-1

Plasmid

#107938

Purpose

cdk-1p::GFP(dpiRNA)::cdk-1::cdk-1 3'UTR construct used for MosSCI in nematode. GFP is optimized by removing all piRNA targeting sites to allow germline expression.

Depositor

Heng-Chi Lee

Article

Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

Insert

GFP(dpiRNA)::CDK-1 (cdk-1 Nematode)

Expression

Worm

Promoter

cdk-1

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DIO-GRAB_CRF1.0

Plasmid

#208662

Purpose

Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in a cre-dependent manner

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0

Use

AAV

Promoter

EFS

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DIO-GRAB_CRFmut

Plasmid

#208663

Purpose

Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) control sensor GRAB_CRFmut in a cre-dependent manner

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut

Use

AAV

Promoter

EFS

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-fMCP

Plasmid

#238908

Purpose

Contains EGFP-fMCP fluorogenic protein

Depositor

Jian-Hui Jiang

Article

Zhou et al Angew Chem Int Ed Engl. 2025 Apr 10:e202

Insert

EGFP-fMCP

Expression

Mammalian

Promoter

miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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