161,697 results

1. pFCiR5

   Plasmid

   #149479

   Purpose

   Broad-Host range expression vector, RSF1010 origin, CinR(AM) activator, PCin promoter, mRFP, Gentamicin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   CinR(AM)-mRFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PCin

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAcRP67A prM/E YFV

   Plasmid

   #52883

   Purpose

   expression of Yellow Fever Virus VLPs in baculovirus

   Depositor

   Yorgo Modis

   Insert

   prM/E
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   May 14, 2014

   Availability

   Academic Institutions and Nonprofits only

3. PPIB

   Plasmid

   #155838

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   PPIB (PPIB Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEBB HA cIAP1 H588A

   Plasmid

   #38233

   Depositor

   Colin Duckett

   Article

   Csomos et al Biochem J. 2009 Apr 28;420(1):83-91.

   Insert

   cIAP1 H588A (BIRC2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   H588A

   Promoter

   EF1a

   Available since

   July 30, 2012

   Availability

   Academic Institutions and Nonprofits only

5. lentiCRISPRv2_AMBRA1#2

   Plasmid

   #174153

   Purpose

   Lentiviral vector expressing Cas9 and a sgRNA against the human AMBRA1 gene

   Depositor

   Julien Sage

   Article

   Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

   Insert

   AMBRA (AMBRA1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJL017

   Plasmid

   #53532

   Purpose

   This plasmid encodes both the etpB and etpA two partner secretion genes on an arabinose-inducible plasmid with myc and 6His tags in-frame with the etpA gene

   Depositor

   James Fleckenstein

   Article

   Fleckenstein et al Nat Protoc. 2009;4(7):1083-92. doi: 10.1

   Insert

   etpBA
   

   Use

   Tags

   6His and myc

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pP2BA

   Plasmid

   #31369

   Depositor

   Aziz Sancar

   Article

   Ye et al J Biol Chem. 2011 May 27. ():.

   Insert

   Per2 (Per2 Mouse)
   

   Use

   Retroviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

8. SERPINC1-bio-His

   Plasmid

   #52076

   Purpose

   Expresses full-length Antithrombin-III precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   SERPINC1 (SERPINC1 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. lentiCRISPRv2_AMBRA1#1

   Plasmid

   #174152

   Purpose

   Lentiviral vector expressing Cas9 and a sgRNA against the human AMBRA1 gene

   Depositor

   Julien Sage

   Article

   Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

   Insert

   AMBRA (AMBRA1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJL030

    Plasmid

    #53533

    Purpose

    supplies EtpC required for optimal EtpA expression on compatible plasmid

    Depositor

    James Fleckenstein

    Article

    Fleckenstein et al Nat Protoc. 2009;4(7):1083-92. doi: 10.1

    Insert

    etpC
    

    Use

    Pacyc184

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

11. TFORF3399

    Plasmid

    #144875

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    POU2F2 (POU2F2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. R701-X71-527: His6-tev-Hs.CALM1(1-80)

    Plasmid

    #159694

    Purpose

    E. coli protein expression of His6-tev-Hs.CALM1(1-80)

    Depositor

    Dominic Esposito

    Article

    Agamasu et al Biophys J. 2019 Mar 19;116(6):1049-1063.

    Insert

    His6-tev-Hs.CALM1(1-80) (CALM1 Human)
    

    Use

    Tags

    His6-tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR221_SLC35F1

    Plasmid

    #132133

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC35F1 (SLC35F1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pC1BA

    Plasmid

    #31368

    Depositor

    Aziz Sancar

    Article

    Ye et al J Biol Chem. 2011 May 27. ():.

    Insert

    Cry1 (Cry1 Mouse)
    

    Use

    Retroviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

15. p-dCas9-HDAC1-H141A-Hygro

    Plasmid

    #104417

    Purpose

    transient expression of dCas9-dHDAC1 fusion protein

    Depositor

    Stephan Beck

    Insert

    dHDAC1 (HDAC1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    H141A

    Promoter

    CMV promoter

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pgex-cIAP1 mut

    Plasmid

    #8334

    Depositor

    Jon Ashwell

    Article

    Li et al Nature 2002 Mar 21;416(6878):345-7.

    Insert

    ciap1mut (BIRC2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    H588A

    Promoter

    Available since

    Nov. 15, 2005

    Availability

    Academic Institutions and Nonprofits only

17. pDONR223_HSD17B10_WT_V5

    Plasmid

    #82941

    Purpose

    Gateway Donor vector containing HSD17B10, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

    Depositor

    Matthew Meyerson, David Root, Jesse Boehm

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    HSD17B10 (HSD17B10 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT4-U6-sgRNA-CMV-EGFP

    Plasmid

    #239587

    Purpose

    Sleeping-beauty based EV for cloning and expression of sgRNAs - concomitant expression of GFP serves as transfection marker

    Depositor

    Andreas Moor

    Article

    Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

    Type

    Empty backbone

    Use

    Sleeping beauty transposone

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. TRAV29/DV5

    Plasmid

    #237986

    Purpose

    Encodes TRAV29/DV5 allele, P2A, and TRBC to generate TCRs via cloning

    Depositor

    Michael Birnbaum

    Insert

    TRBC-P2A-TRAV29/DV5 (TRAV29DV5 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TRAV26-2

    Plasmid

    #237984

    Purpose

    Encodes TRAV26-2 allele, P2A, and TRBC to generate TCRs via cloning

    Depositor

    Michael Birnbaum

    Insert

    TRBC-P2A-TRAV26-2 (TRAV26-2 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge