Addgene

We narrowed to 5,794 results for: RIK

Showing: 3521 - 3560 of 5794 results

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pCMV-iRFP713-ORP9-PH

Plasmid

#214269

Purpose

Mammalian expression of near-IR fluorescent PI4P reporter iRFP713-ORP9-PH

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

iRFP713-ORP9(1-111) (Osbpl9 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS469-pLK.O1-SpySgRNA/DTS13-Telomere

Plasmid

#85715

Purpose

Telomere-targeting Spy sgRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Insert

telomere sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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Stat3 Flag pRc/CMV

Plasmid

#8707

Depositor

Jim Darnell

Article

Horvath et al Genes Dev 1995 Apr 15;9(8):984-94.

Insert

Stat3 (Stat3 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

*see comments

Promoter

Available since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only

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pFudio-Cpg15-IRES2-TdTomatoW

Plasmid

#134309

Purpose

Expresses cpg15 and TdTomato upon coexpression with Cre recombinase

Depositor

Elly Nedivi

Article

Subramanian et al Cell Rep. 2019 Aug 6;28(6):1584-1595.e5.

Inserts

cpg15 (Nrn1 Mouse, Rat)
TdTomato

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

UbC

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-dTomato-ORP9-PH

Plasmid

#214268

Purpose

Mammalian expression of red fluorescent PI4P reporter dTomato-ORP9-PH

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

dTomato-ORP9(1-111) (Osbpl9 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2423_Nme2.Smu-ABE8e-i7

Plasmid

#201521

Purpose

Mammalian expression of domain inlaid Nme2.Smu-ABE8e-i7

Depositor

Erik Sontheimer

Article

Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

Insert

Nme2.Smu-ABE8e-i7

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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PX459-sgRNA048_Grb10

Plasmid

#232934

Purpose

Expression vector for a sgRNA against the mouse Grb10 locus and SpCas9 with T2A-Puromycin resistance gene.

Depositor

Danny Reinberg

Article

Lucero et al bioRxiv, March 19, 2025

Insert

Cas9-T2A-PuroR (Grb10 S. pyogenes)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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PLEXIN A1

Plasmid

#58237

Purpose

Riboprobe for in vitro transcription of Plexin A1

Depositor

Christiana Ruhrberg

Article

Bouvree et al Circ Res. 2012 Aug 3;111(4):437-45. doi:

Insert

Plexin A1 (Plxna1 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
Puro-iNotch1IC

Plasmid

#75338

Purpose

doxycycline inducible Notch1 intracellular domain gene over-expression

Depositor

Danwei Huangfu

Article

Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

Insert

Notch1 Intracellular domain (Notch1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE-tight

Available since

May 24, 2016

Availability

Academic Institutions and Nonprofits only

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EGFP-IC2-FL

Plasmid

#51409

Purpose

expression of EGFP-tagged full-length dynein intermediate chain IC2C in mammalian cells

Depositor

Trina Schroer

Article

King et al Mol Biol Cell. 2003 Dec;14(12):5089-97.

Insert

dynein intermediate chain IC2C (Dync1i2 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG2IG-Hmga2

Plasmid

#122294

Purpose

Overexpression for mouse Hmga2

Depositor

Yukiko Gotoh

Article

Kishi et al Nat Neurosci. 2012 Aug;15(8):1127-33. do

Insert

Hmga2 (Hmga2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-BG505.SOSIP-gp160

Plasmid

#123273

Purpose

Mammalian expression plasmid for Env from the BG505 HIV-1 isolate (containing SOSIP mutations)

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BG505) Env

Use

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; SOSIP mutations

Promoter

CMV

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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pEJS613_pTetR-P2A-BFPnls/sgTelo

Plasmid

#108649

Purpose

Telomere-targeting Spy sgRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Inserts

Telomere-targeting sgRNA
TetR-P2A-BFP

Use

CRISPR and Lentiviral

Tags

No

Expression

Mammalian

Mutation

Promoter

U6 and hPGK

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pSFFV-dNme2Cas9

Plasmid

#193190

Purpose

Lentiviral vector expressing nuclease-dead Cas9 from Neisseria meningitis (dNme2)

Depositor

Erik Sontheimer

Article

Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

Insert

dNme2Cas9

Use

Tags

NLS and NLS-3xHA-NLS

Expression

Mammalian

Mutation

H588A, D16A

Promoter

SFFV

Available since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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Cas9-GFP_sg_mAMPKa1

Plasmid

#79004

Purpose

Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 1.

Depositor

Reuben Shaw

Article

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Insert

Prkaa1 (Prkaa1 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pCEP4-intron-BaLgp160

Plasmid

#100918

Purpose

Mammalian expression plasmid for Env from the BaL HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BaL) Env with upstream intron

Use

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pNCS tdTomato

Plasmid

#91767

Purpose

Bacterial expression of a fluorescent protein, tandem dimer Tomato (tdTomato)

Depositor

Erik Rodriguez, Roger Tsien

Article

Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

Insert

tandem dimer Tomato

Use

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-Kdm6b(1025-End)-pA

Plasmid

#100278

Purpose

In vitro transcription template (T7 promoter) for Kdm6b catalytic domain active form.

Depositor

Yi Zhang

Article

Inoue et al Nature. 2017 Jul 19. doi: 10.1038/nature

Insert

Kdm6b (Kdm6b Mouse)

Use

Tags

Flag epitope tag

Expression

Mammalian

Mutation

catalytic domain (1025aa-End)

Promoter

T7

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pEJS2422_Nme2.Smu-ABE8e-i1

Plasmid

#201520

Purpose

Mammalian expression of domain inlaid Nme2.Smu-ABE8e-i1

Depositor

Erik Sontheimer

Article

Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

Insert

Nme2.Smu-ABE8e-i1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCEP4-Q842gp160

Plasmid

#100931

Purpose

Mammalian expression plasmid for Env from the Q842.d12 HIV-1 strain

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (Q842.d12) Env

Use

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCIG2-Flag-mNuak1 WT

Plasmid

#168507

Purpose

For expression of Nuak1 (AMPK-related kinase also called ARK5/Omphk1)

Depositor

Franck Polleux

Article

Courchet et al Cell. 2013 Jun 20;153(7):1510-25. doi: 1

Insert

Nuak1 (Nuak1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-mTagBFP2-ORP9-PH

Plasmid

#214267

Purpose

Mammalian expression of blue fluorescent PI4P reporter mTagBFP2-ORP9-PH

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

mTagBFP2-ORP9(1-111) (Osbpl9 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Cas9-GFP_sg_mAMPKa2

Plasmid

#79005

Purpose

Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 2.

Depositor

Reuben Shaw

Article

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Insert

Prkaa2 (Prkaa2 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pF(UG) hSyn SYT7alpha-HaloTag

Plasmid

#179715

Purpose

Lentiviral expression of mouse Syt7 with C terminal HaloTag

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYT7 (Syt7 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmpr2

Plasmid

#163403

Purpose

Expresses BMPR-2 in mammalican cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRE-tight2-Tmem98-FLAG

Plasmid

#124506

Purpose

Expresses FLAG-tagged mouse TMEM98 in mammalian cells in a doxycycline-dependent manner (C-terminal tag)

Depositor

Renee van Amerongen

Article

van der Wal et al PLoS One. 2020 Jan 21;15(1):e0227435. do

Insert

Tmem98 (Tmem98 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

tetO-CMV

Available since

May 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEJS2424_Nme2.Smu-ABE8e-i8

Plasmid

#201522

Purpose

Mammalian expression of domain inlaid Nme2.Smu-ABE8e-i8

Depositor

Erik Sontheimer

Article

Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

Insert

Nme2.Smu-ABE8e-i8

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-h-mmPtch1-FL

Plasmid

#120889

Purpose

Expresses wildtype Ptch1 for function assays

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

ptch1 (Ptch1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
piggybac-EF1-H2AZ-halo

Plasmid

#197907

Purpose

single-particle tracking for H2AZ

Depositor

James Zhe Liu

Article

Dong et al bioRxiv 2022.10.26.513917

Insert

H2az2 (H2az2 Mouse)

Use

Tags

Halotag

Expression

Mammalian

Mutation

Promoter

EF1

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Nanog-2A-GFP

Plasmid

#59994

Purpose

Inserts GFP immediately downstream of the Nanog coding region

Depositor

Rudolf Jaenisch

Article

Faddah et al Cell Stem Cell. 2013 Jul 3;13(1):23-9. d

Insert

Nanog (Nanog Mouse)

Use

Mouse Targeting

Tags

2A-GFP

Expression

Mutation

Promoter

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAX Itgb1-FLAG

Plasmid

#30153

Depositor

Tracy Young-Pearse, Dennis Selkoe

Article

Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

Insert

integrin beta 1 (Itgb1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2011

Availability

Academic Institutions and Nonprofits only
pCEP4-BaLgp140-TM

Plasmid

#111843

Purpose

Mammalian expression plasmid for extracellular gp140 from the BaL HIV-1 strain fused to a transmembrane tether

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BaL) Env

Use

Tags

CD5 leader sequence and TM helix from MHC class I

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcdna3 flag - ULK2 wt

Plasmid

#27637

Depositor

Reuben Shaw

Article

Egan et al Science. 2010 Dec 23. ():.

Insert

mouse ULK2 (Ulk2 Mouse)

Use

Tags

flag

Expression

Mammalian

Mutation

Initial M removed from ULK2.

Promoter

Available since

March 16, 2011

Availability

Academic Institutions and Nonprofits only
mTagBFP2-H3.3-N-14

Plasmid

#55302

Purpose

Localization: Nucleus/Histones, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

H3.3 (H3f3b Mouse)

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pYLL201

Plasmid

#53454

Purpose

Expresses HA-tagged mouse DNA topoisomerase IIbeta (full length cDNA, in pCS2+ backbone)

Depositor

Leroy Liu, Lisa Lyu

Article

Ban et al Mol Cell Biol. 2013 Oct;33(20):4008-16.

Insert

HA-tagged Mus musculus topoisomerase (DNA) II beta (Top2b) (Top2b Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
Nanog-2A-mCherry

Plasmid

#59995

Purpose

Inserts mCherry immediately downstream of the Nanog coding region

Depositor

Rudolf Jaenisch

Article

Faddah et al Cell Stem Cell. 2013 Jul 3;13(1):23-9. d

Insert

Nanog (Nanog Mouse)

Use

Mouse Targeting

Tags

2A-mCherry

Expression

Mutation

Promoter

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
NFkBr-IL1Ra

Plasmid

#183065

Purpose

Expresses IL-1Ra downstream of a synthetic promoter activated by the NFkB signaling pathway

Depositor

Farshid Guilak

Article

Pferdehirt et al Tissue Eng Part A. 2019 May;25(9-10):809

Insert

interleukin-1 receptor antagonist (Il1rn Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Nme2Cas9_pCDest

Plasmid

#119923

Purpose

Nme2Cas9 CMV-driven mammalian expression plasmid

Depositor

Erik Sontheimer

Article

Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

Insert

Nme2Cas9

Use

Tags

NLS-3xHA-NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSc + MShal

Plasmid

#16166

Depositor

Larry Salkoff

Article

Pak et al Proc Natl Acad Sci U S A. 1991 May 15. 8

Insert

Mus musculus potassium voltage-gated channel (Kcnd1 Mouse)

Use

Xenopus oocyte expression

Tags

Expression

Mutation

N/A

Promoter

Available since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
pCFJ1209

Plasmid

#44491

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1 [Peft-3 tdTomato(NLS) tbb-2 UTR, NeoR]

Use

Gateway expression clone

Tags

Expression

Mutation

Promoter

Available since

April 29, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge

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