We narrowed to 7,278 results for: cas9 plasmid

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pX459-sg-mGreenLantern332

Plasmid

#179914

Purpose

All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern fluorescent protein site 332.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

mGreenLantern sgRNA

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

U6

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pEG302 22aa SunTag VP64 gRNA17 (FWA)

Plasmid

#120250

Purpose

CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA

Depositor

Steven Jacobsen

Article

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

g17_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pSECC

Plasmid

#60820

Purpose

3rd generation vector. Expresses a sgRNA of interest, Cas9 and Cre

Depositor

Tyler Jacks

Article

Sanchez-Rivera et al Nature. 2014 Oct 22. doi: 10.1038/nature

Inserts

Cas9
Cre

Use

CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

Tags

Flag

Expression

Mammalian

Mutation

BsmBI site eliminated by C->A; E308G

Promoter

EFS and EFS (after Cas9-2A)

Available Since

Nov. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pL-CRISPR.SFFV.GFP

Plasmid

#57827

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. SFFV Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
SFFV
P2A-eGFP

Use

CRISPR and Lentiviral

Tags

FLAG

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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MSP469

Plasmid

#65771

Purpose

Human expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant)

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Mammalian

Mutation

D1135V, R1335Q, and T1337R mutations in Cas9

Promoter

CMV & T7

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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MSP680

Plasmid

#65772

Purpose

Human expression vector for SpCas9 EQR variant: CMV-T7-humanSpCas9(D1135E/R1335Q/T1337R)-NLS-3xFLAG (EQR variant)

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E/R1335Q/T1337R)-NLS-3XFlag

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Mammalian

Mutation

D1135E, R1335Q, and T1337R mutations in Cas9

Promoter

CMV & T7

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-SCP1-dSa VPR mini.-2X snRP-1 Hbb

Plasmid

#99692

Purpose

Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Hbb, vector allows for strong activation of mouse Hbb, can be packaged and delivered as AAV

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9 and gRNA targeting Hbb (Hbb Synthetic)

Use

AAV

Tags

VPR mini

Mutation

dead Cas9

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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HCP9

Plasmid

#166111

Purpose

This plasmid encodes a Cas9 protein as well as a sgRNA that targets the C-terminus of Hta2

Depositor

Matthias Heinemann

Insert

Hta2-sg18 (HTA2 Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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psBIG1a

Plasmid

#229957

Purpose

bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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psBIG1b

Plasmid

#229958

Purpose

bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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psBIG2abc

Plasmid

#229963

Purpose

bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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psBIG2ab

Plasmid

#229962

Purpose

bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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psBIG2abcd

Plasmid

#229964

Purpose

bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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psBIG2abcde

Plasmid

#229965

Purpose

bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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psBIG1d

Plasmid

#229960

Purpose

bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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psBIG1e

Plasmid

#229961

Purpose

bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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psBIG1c

Plasmid

#229959

Purpose

bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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OgeuIscB CTG RNA

Plasmid

#222865

Purpose

This plasmid codes for the guide of the OgeuIscB with a CTG target

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Insert

OgeuIscB omega RNA with a (CTG)6 target sequence

Use

CRISPR

Expression

Mammalian

Promoter

Human U6

Available Since

Oct. 17, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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OgeuIscB GCT RNA

Plasmid

#222862

Purpose

This plasmid codes for the guide of the OgeuIscB with a GCT target

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Insert

OgeuIscB omega RNA with a (GCT)6 target sequence

Use

CRISPR

Expression

Mammalian

Promoter

Human U6

Available Since

Oct. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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OgeuIscB GCA RNA

Plasmid

#222864

Purpose

This plasmid codes for the guide of the OgeuIscB with a GCA target

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Insert

OgeuIscB omega RNA with a (GCA)6 target sequence

Use

CRISPR

Expression

Mammalian

Promoter

Human U6

Available Since

Oct. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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