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pET21-pelB-LaTdT-39

Plasmid

#228980

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-39, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-39

Use

Tags

6xHIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_SM-FLuc0-malat1

Plasmid

#210580

Purpose

Expresses SM(FLAG)-nonoptimal Firefly - 3'UTR malat1 triple helix

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-nonoptimal FLuc - 3'UTR malat1 triple helix

Use

Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_SM-FLuc100-malat1

Plasmid

#210581

Purpose

Expresses SM(FLAG)-optimal Firefly luciferase - 3'UTR malat1 triple helix

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-optimal FLuc - 3'UTR malat1 triple helix

Use

Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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CIRTS-YTHDF2-Malat1

Plasmid

#216858

Purpose

CIRTS RNA targeting system for targeted knockdown of m6A-containing Malat1 at the synapse. CIRTS-YTHDF2-Calm3 and Malat1 gRNA is expressed under human Syn1 and U6 promoter, respectively.

Depositor

Timothy Bredy

Article

Madugalle et al J Neurosci. 2023 Oct 25;43(43):7084-7100

Insert

CIRTS-YTHDF2-Calm3-U6-Malat1 gRNA

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Syn1, U6

Available since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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CIRTS-FTO-Malat1

Plasmid

#216860

Purpose

CIRTS RNA targeting system for targeted removal of m6A on Malat1 at the synapse. CIRTS-FTO-Calm3 and Malat1 gRNA is expressed under human Syn1 and U6 promoter, respectively.

Depositor

Timothy Bredy

Article

Madugalle et al J Neurosci. 2023 Oct 25;43(43):7084-7100

Insert

CIRTS-FTO-Calm3-U6-Malat1 gRNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Syn1, U6

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-sgLats1#2/Cre

Plasmid

#173600

Purpose

Expresses a Lats1-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Lats1 (Lats1 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgLats1#1/Cre

Plasmid

#173599

Purpose

Expresses a Lats1-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Lats1 (Lats1 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pPD152 Reporter Template v2

Plasmid

#138752

Purpose

Template for COMET promoters, expresses EYFP reporter upon promoter activation. BsaI sites produce palindromic sticky ends.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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Hy_pMtnA eGFP MALAT1

Plasmid

#132643

Purpose

Expresses an eGFP mRNA from the Drosophila Metallothionein A promoter that ends in the MALAT1 triple helix

Depositor

Jeremy Wilusz

Article

Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

Insert

eGFP

Use

Tags

Expression

Insect

Mutation

Promoter

MtnA

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3-cytXlATL2-R232Q

Plasmid

#124064

Purpose

Control protein. The R232Q mutation abolishes dimerization activity, so this construct is inactive compared to wild type cytATL

Depositor

Tom Rapoport

Article

Wang et al J Cell Biol. 2013 Dec 9;203(5):801-14. d

Insert

cytXlATL2-R232Q

Use

Tags

Expression

Bacterial

Mutation

Promoter

tac

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pRRG245-Ras-related

Plasmid

#99079

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Ras-related (also known as h.18.4a)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

Ras-related (also known as h.18.4a) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pDECKO-mCherry MALAT1_Enhancer.4

Plasmid

#78539

Purpose

Experimental plasmid

Depositor

Rory Johnson, Roderic Guigo

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 and U6 (for expressing sgRNA)

Available since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pDECKO-mCherry MALAT1_Enhancer.3

Plasmid

#78538

Purpose

Experimental plasmid

Depositor

Rory Johnson, Roderic Guigo

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 and U6 (for expressing sgRNA)

Available since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pDECKO-mCherry MALAT1_Exon.3

Plasmid

#78542

Purpose

Experimental plasmid

Depositor

Rory Johnson, Roderic Guigo

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 and U6 (for expressing sgRNA)

Available since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pDECKO-mCherry MALAT1_Enhancer.2

Plasmid

#78537

Purpose

Experimental plasmid

Depositor

Rory Johnson, Roderic Guigo

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 and U6 (for expressing sgRNA)

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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mGeos2-VEL-Clathrin-15

Plasmid

#57544

Purpose

Localization: Clathrin Vesicles, Excitation: 503 / 569, Emission: 514 / 581

Depositor

Michael Davidson

Insert

Clathrin (CLTB Human)

Use

Tags

mGeos2-VEL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pORANGE Cloning template vector

Plasmid

#131471

Purpose

Cloning template form ORANGE method based knock-in constructs

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Type

Empty backbone

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP_mNG2(11)_Clathrin light chain

Plasmid

#82608

Purpose

Expresses mNG2(11) tagged clathrin light chain in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

mNG2(11)_Clathrin light chain

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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SpCas9-TadCBEd-ivt-template

Plasmid

#193843

Purpose

Template for in vitro transcription of TadCBEd (with SpCas9)

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadCBEd with SpCas9

Use

In vitro transcription template

Tags

Expression

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pZDonor rosa26 LSL-mMALAT1

Plasmid

#140691

Purpose

generate mouse MALAT1 conditional transgenic mice using CRISPR system targeting mouse Rosa 26 region

Depositor

Jianjun Zhao

Insert

mouse MALAT1 (Malat1 Mouse)

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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