Addgene

We narrowed to 11,673 results for: otos

Showing: 2671 - 2700 of 11673 results

Results per page:

Sort results by:

BoNT/A-LC

Plasmid

#31602

Depositor

Axel Brunger

Article

Zuniga et al Structure. 2008 Oct 8. 16(10):1588-97.

Insert

BoNT/A LC

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
LRG2.1-TagBFP2

Plasmid

#124773

Purpose

Lentiviral plasmid to co-express a guide RNA and TagBFP2

Depositor

Jason Sheltzer

Article

Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pPT24-mtnluc

Plasmid

#184339

Purpose

Transformation of mitochondrial DNA in yeast

Depositor

Martin Ott

Article

Rzepka et al STAR Protoc. 2022 May 25;3(2):101359. do

Insert

nanoluciferase recoded for mitochondrial expression flanked by COX2 UTRs

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLZ12Km2-P23R:TA:Xen5

Plasmid

#88902

Purpose

Toxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expression

Depositor

Thomas Proft

Article

Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2

Insert

toxin-antitoxin + LuxABCDE

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only
pMRX-No-HaloTag7-LC3

Plasmid

#184900

Purpose

Stable expression of HaloTag7-LC3 in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

LC3B

Use

Tags

HaloTag7

Expression

Mammalian

Mutation

Promoter

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJMP2834

Plasmid

#160673

Purpose

Mobile CRISPRi sfGFP 'test', gmc6 sgRNA

Depositor

Jason Peters

Article

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Insert

gmc6 (gfp) sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV-U6gRNA-EF(BbsI)-PGKpuro2ABFP

Plasmid

#62348

Purpose

An empty optimized gRNA expression vector

Depositor

Hiroshi Ochiai

Article

Ochiai et al Nucleic Acids Res. 2015 Jun 19. pii: gkv

Insert

none

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-Mito-HyPerGRt

Plasmid

#173198

Purpose

AAV transfer plasmid for a HyPer7 and tdTomato fusion under hSyn promoter

Depositor

Huiwang Ai

Article

Li et al ACS Sens. 2022 Feb 28. doi: 10.1021/acss

Insert

Hyper7-tdTomato

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
RTB300

Plasmid

#80410

Purpose

human cTNT minigene with genomic segment containing alternative exon 5

Depositor

Thomas Cooper

Article

Philips et al Science. 1998 May 1;280(5364):737-41.

Insert

TNNT2 (TNNT2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

RSV

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pEiLOV-N1

Plasmid

#26588

Depositor

John Christie

Article

Chapman et al Proc Natl Acad Sci U S A. 2008 Dec 16. 1

Insert

iLOV

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 28, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA TrkB

Plasmid

#24088

Depositor

Moses Chao

Article

Yano et al J Neurosci. 2001 Feb 1. 21(3):RC125.

Insert

TrkB (Ntrk2 Rat)

Use

Tags

Expression

Mammalian

Mutation

R133I

Promoter

Available since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSET FLIPPi-5u

Plasmid

#13554

Depositor

Wolf Frommer

Article

Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

Insert

FLIPPi S52A

Use

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

S52A in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pPSmOrange-C1

Plasmid

#31899

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

Insert

PSmOrange

Use

Tags

Expression

Mammalian

Mutation

S21T, Q63L, F100Y, L125M, K166R, P192S (mutations…

Promoter

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP SV2A GFP

Plasmid

#218544

Purpose

Mamallian Expression of SV2A GFP

Depositor

Edwin Chapman

Article

Dong et al Science. 2006 Apr 28;312(5773):592-6. do

Insert

SV2A

Use

Tags

Expression

Mammalian

Mutation

S42F

Promoter

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
sgBbsI (p2Tol-U6-2xBbsI-sgRNA-HygR)

Plasmid

#71485

Purpose

Base plasmid for sgRNA cloning with Hygromycin resistance and Tol2 transposon arms

Depositor

Richard Sherwood

Article

Arbab et al Stem Cell Reports. 2015 Oct 27. pii: S22

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

U6

Available since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pRha-rsw-EYFP

Plasmid

#182928

Purpose

EYFP driven by inducible promoter Prha-rsw

Depositor

Himadri Pakrasi

Article

Liu et al ACS Synth Biol. 2020 Jun 19;9(6):1441-14

Insert

EYFP

Use

Tags

Expression

Mutation

Promoter

Prha-rsw

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet

Plasmid

#130509

Purpose

expresses mScarlet

Depositor

Dorus Gadella

Article

Bindels et al Nat Protoc. 2020 Feb;15(2):450-478. doi:

Insert

mScarlet

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBGK

Plasmid

#65796

Purpose

Vector for Cas9 mRNA synthesis

Depositor

Masato Ohtsuka

Article

Harms et al Curr Protoc Hum Genet. 2014 Oct 1;83:15.

Insert

Cas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
sspB-mSc-sspB

Plasmid

#114422

Purpose

Translocating to iLID domain

Depositor

Dorus Gadella

Article

Van Geel et al Small GTPases. 2018 Sep 5:1-10. doi: 10.

Insert

iLID domain

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD3-proD/Lux-nat

Plasmid

#120324

Purpose

Bacterial luminescence

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

Lux operon

Use

Tags

Expression

Bacterial

Mutation

Promoter

proD

Available since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMA3211

Plasmid

#46879

Purpose

Lentiviral vector for Tet-inducible expression. Puromycin-resistance

Depositor

Troy Stevens, Mikhail Alexeyev

Article

Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TRE-Tight

Available since

Aug. 14, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pBTK800

Plasmid

#179209

Purpose

GFP dropout for assembling a double-stranded RNA expression plasmid

Depositor

Jeffrey Barrick

Article

Lariviere et al Nat Protoc. 2023 Mar;18(3):902-928. doi:

Insert

GFP dropout

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMK436 (mAID-mCherry-P2A-Hygro)

Plasmid

#168231

Purpose

C-terminal tagging with mAID-mCherry

Depositor

Masato Kanemaki

Article

Saito et al Curr Protoc. 2021 Aug;1(8):e219. doi: 10

Insert

mAID-mCherry-P2A-Hygro

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJMP2832

Plasmid

#160672

Purpose

Mobile CRISPRi sfGFP 'test', gmc6 sgRNA

Depositor

Jason Peters

Article

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Insert

gmc6 (gfp) sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCB1532S-RFP

Plasmid

#101856

Purpose

Golden-Gate compatible fungal transformation vector carrying Sulfonylurea Resistance (Chlorimuron Ethyl) with RFP screening cassette (RFP knocked out by GG reaction, allowing for red-white screening)

Depositor

Sophien Kamoun

Article

Pennington et al Figshare (2017)

Type

Empty backbone

Use

Fungal expression plasmid, tested in magnaporthe

Tags

Expression

Mutation

Promoter

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pH2B-PSmOrange

Plasmid

#31920

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

Insert

PSmOrange

Use

Tags

H2B

Expression

Mammalian

Mutation

S21T, Q63L, F100Y, L125M, K166R, P192S in PSmOran…

Promoter

Available since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI 9.0

Plasmid

#74229

Purpose

Luminescent coincidence reporter with FLAG tags, FLuc-P2A-NLucPEST

Depositor

James Inglese

Article

Schuck et al Curr Protoc Neurosci. 2017 Apr 10;79:5.3

Insert

3xFlag-FLuc-P2A-3xFlag-NLucPEST

Use

Tags

Expression

Mammalian

Mutation

Promoter

None

Available since

May 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti_CMV_ChR2-3M-YFP

Plasmid

#186977

Purpose

Expresses ChR2-3xM channelrhodopsin in mammalian cells

Depositor

Adam Cohen

Article

Hayward et al eLife. 2023 Oct 6;12:RP86833. doi: 10.75

Insert

ChR2-3xM-YFP

Use

Lentiviral

Tags

eYFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDRESS_mTurquoise2_spatial-linker-P2A_mScarletI

Plasmid

#130510

Purpose

expresses mScarlet1

Depositor

Dorus Gadella

Article

Bindels et al Nat Protoc. 2020 Feb;15(2):450-478. doi:

Insert

mScarlet1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC18-mini-Tn7T-Gm-LacZ20

Plasmid

#65027

Purpose

GmR mini-Tn7 vector, for construction of B-galactosidase protein fusions, frame 2. See Depositor Comments below for information regarding the lacZ sequence.

Depositor

Herbert Schweizer

Article

Choi et al Nat Protoc. 2006;1(1):153-61.

Type

Empty backbone

Use

Tags

lacZ

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge