We narrowed to 5,867 results for: chia

1. pET303-colE1T

   Plasmid

   #155180

   Purpose

   Expresses colicin E1 residues 1-190

   Depositor

   Joanna Slusky

   Article

   Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

   Insert

   colicin E1 residues 1-190
   

   Use

   Tags

   C-terminal 6x Histidine tag

   Expression

   Bacterial

   Mutation

   truncation of colicin E1 including 1-190

   Promoter

   T7

   Available since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEvoltac EcW-TGA-h9

   Plasmid

   #218765

   Purpose

   encodes EcWRS-h9 and EcW-tRNA-TGA

   Depositor

   Abhishek Chatterjee

   Article

   Italia et al Nat Chem Biol. 2017 Apr;13(4):446-450. d

   Insert

   EcWRS-h9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   S8A, V144S, V146A

   Promoter

   tacI

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAC-BETAipi

   Plasmid

   #53277

   Purpose

   Contains ctrE, crtB, crtI, crtY, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coli

   Depositor

   Francis X Cunningham Jr

   Article

   Cunningham FX et al Plant J. 2005 Feb;41(3):478-92.

   Insert

   idi
   

   Use

   Low copy number bacterial cloning vector

   Tags

   Expression

   Mutation

   Promoter

   endogenous promoter

   Available since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1 FLII12Pglu-700uDelta6

   Plasmid

   #17866

   Depositor

   Wolf Frommer

   Article

   Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

   Insert

   FLII12Pglu-700uDelta6
   

   Use

   Tags

   Citrine

   Expression

   Mammalian

   Mutation

   700uDelta6, ECFP insert in mglB

   Promoter

   Available since

   April 29, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. ykgMO-IGS-LuxCDABE

   Plasmid

   #207232

   Purpose

   Calprotectin sensing promoter with luciferase reporter

   Depositor

   Arthur Prindle

   Article

   Xia et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

   Insert

   ykgMO-IGS
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pG108 - K

   Plasmid

   #178039

   Purpose

   Bacteroides - Escherichia coli shuttle vector with TetQ and Kanamycin selection markers

   Depositor

   Janina Lewis

   Article

   Jones et al Mol Oral Microbiol. 2020 Aug;35(4):181-1

   Insert

   Kanamycin resistance
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   ErmAM has been replaced with Kanamycin for select…

   Promoter

   Available since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAC-LYCipi

   Plasmid

   #53279

   Purpose

   Contains crtE, crtB, crtI, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces lycopene in Escherichia coli

   Depositor

   Francis X Cunningham Jr

   Article

   Cunningham FX et al Eukaryot Cell. 2007 Mar;6(3):533-45. Epu

   Insert

   idi
   

   Use

   Low copy number bacterial cloning vector

   Tags

   Expression

   Mutation

   Promoter

   endogenous promoters

   Available since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRM006

   Plasmid

   #98922

   Purpose

   Express Histag-SUMO-FtsZ for purification in E coli

   Depositor

   Jie Xiao

   Article

   Yang et al Science. 2017 Feb 17;355(6326):744-747.

   Insert

   FtsZ
   

   Use

   Tags

   Histag-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTuLys2CMR2

   Plasmid

   #53544

   Purpose

   Inhibition module. Constructed from the parent plasmids pLuxRI, pLuxmCherry, pET15bLCFPT7, and pLysS. The plasmid can express T7 lysozyme by inducing AHL and LuxR. Chloramphenicol resistance

   Depositor

   Lingchong You

   Article

   Payne et al Mol Syst Biol. 2013 Oct 8;9:697. doi: 10

   Insert

   pLux
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MscL(G22S)-c-term-cpGFP-ERexp

    Plasmid

    #195346

    Purpose

    Control plasmid for the MscL tension biosensor

    Depositor

    Allen Liu

    Article

    Hsu et al ACS Sens. 2023 Jan 27;8(1):12-18. doi: 1

    Insert

    MscL(G22S)-c-term-cpGFP-ERexp
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMVd2

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCDB302

    Plasmid

    #113673

    Purpose

    expresses Cth SUMO protease in Escherichia coli

    Depositor

    Christopher Bahl

    Article

    Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

    Insert

    Cth SUMO protease
    

    Use

    Tags

    10-His

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-934

    Promoter

    T7

    Available since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLV-mNeon-TurboID

    Plasmid

    #186610

    Purpose

    Third generation lentiviral vector expressing mNeonGreen fused to V5-TurboID

    Depositor

    Gia Voeltz

    Article

    Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

    Insert

    mNeon-V5-TurboID
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    For reference see Branon et al., 2018

    Promoter

    CMV

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pVTU_260_BirA

    Plasmid

    #63682

    Purpose

    Encodes E. coli biotin ligase gene (BirA) for expression in yeast cells

    Depositor

    Aaron Hoskins

    Article

    Rodgers et al RNA. 2015 May;21(5):1031-41. doi: 10.126

    Insert

    BirA
    

    Use

    Tags

    6X Histidine and TEV cleavage site

    Expression

    Yeast

    Mutation

    Promoter

    ADH1/yeast

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato

    Plasmid

    #60658

    Purpose

    Flp-dependent expression of mNaChBac-T2A-tdTomato

    Depositor

    Massimo Scanziani

    Article

    Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

    Has Service

    AAV8

    Insert

    FLEX-mNaChBac-T2A-tdTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1α

    Available since

    Nov. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPL5606_TEF1*-Cre_ADE2

    Plasmid

    #60932

    Purpose

    Constitutive expression vector for Cre-recombinase containing ADE2 selection marker.

    Depositor

    Robert Piper

    Article

    MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

    Insert

    Cre
    

    Use

    Tags

    Driven from a mutant TEF1 promoter with 0.16x act…

    Expression

    Yeast

    Mutation

    Promoter

    mutant TEF1

    Available since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPL5608_TEF1*-Cre_TRP1

    Plasmid

    #60931

    Purpose

    Constitutive expression vector for Cre-recombinase containing TRP1 selection marker.

    Depositor

    Robert Piper

    Article

    MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

    Insert

    Cre
    

    Use

    Tags

    Driven from a mutant TEF1 promoter with 0.16x act…

    Expression

    Yeast

    Mutation

    Promoter

    Mutant TEF1

    Available since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PT7lacO-His6-AlaRS

    Plasmid

    #224362

    Purpose

    Express AlaRS protein for his-tagged purification

    Depositor

    Richard Murray

    Article

    Zhang et al bioRxiv 2024.06.19.599772

    Insert

    AlaRS (alaS E. coli)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCHA1.1-Rap2a-V5-Hygro

    Plasmid

    #195510

    Purpose

    lentiviral vector expressing RAP2A CDS with hygromycin selection

    Depositor

    Joanna Wysocka

    Article

    Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

    Insert

    Hygromycin
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPL5071_TEF1*-Cre_URA3

    Plasmid

    #60930

    Purpose

    Constitutive expression vector for Cre-recombinase containing URA3 selection marker.

    Depositor

    Robert Piper

    Article

    MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

    Insert

    Cre
    

    Use

    Tags

    Driven from a mutant TEF1 promoter with 0.16x act…

    Expression

    Yeast

    Mutation

    Promoter

    mutant TEF1

    Available since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBEL1994

    Plasmid

    #139898

    Purpose

    Expression plasmid for LetB PLL2 deletion (without TM region)

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetB(43-877; delta229-239, D228G, L240G)-6xHis

    Promoter

    Available since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBEL2511

    Plasmid

    #195625

    Purpose

    Complementation of MlaA(WT)

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-EF1α-F-FLEX-mNaChBacMut-T2A-tdTomato

    Plasmid

    #60660

    Purpose

    Flp-dependent expression of mNaChBacMut-T2A-tdTomato

    Depositor

    Massimo Scanziani

    Article

    Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

    Insert

    FLEX-mNaChBacMut-T2A-tdTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    E191K

    Promoter

    EF1α

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28a-mCherryGGBsMTS

    Plasmid

    #133625

    Purpose

    Expression vector for mCherry fused to a membrane targeting sequence (MTS) of the B.subtilis MinD (C-terminal fusion) and an N-terminal His-tag for purification.

    Depositor

    Petra Schwille

    Article

    Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

    Insert

    mCherryGGBsMTS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. ykgMO-IGS-sfGFP

    Plasmid

    #207231

    Purpose

    Calprotectin sensing promoter with sfGFP reporter

    Depositor

    Arthur Prindle

    Article

    Xia et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

    Insert

    ykgMO-IGS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. SurA

    Plasmid

    #213138

    Purpose

    Expresses chaperone SurA

    Depositor

    Sebastian Hiller

    Article

    Rath et al Nat Commun. 2023 Sep 13;14(1):5648. doi:

    Insert

    SurA (21-428) (surA E.coli)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Beta-Lactamase

    Plasmid

    #62729

    Purpose

    Expresses Beta-Lactamase protein in bacterial cells

    Depositor

    Niels Geijsen

    Article

    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

    Insert

    Beta-Lactamase
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Deletion of periplasmic signal domain

    Promoter

    T7

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pPB-R1R2-EM7neoPheS

    Plasmid

    #112923

    Purpose

    piggyBac Transposon vector with a Gateway destination cassette (neoPheS selection)

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    neoPheS
    

    Use

    Piggybac transposon gateway destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAC-GAMMAipi

    Plasmid

    #53278

    Purpose

    Has ctrE, crtB, crtI, crtY, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and crtYm gene of marine bacterium P99-3. and produces gamma-carotene in Escherichia coli

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Plant J. 2005 Feb;41(3):478-92.

    Insert

    crtYm
    

    Use

    Low copy number bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    endogenous promoter

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRAF-MutL

    Plasmid

    #86607

    Purpose

    E. coli MutL expression plasmid

    Depositor

    Richard Fishel

    Article

    Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

    Insert

    E. coli MutL
    

    Use

    Tags

    his-tag and ald-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p177bp-LacOx162-Hyg

    Plasmid

    #210215

    Purpose

    Integrates 162xLacO repeats with a hygromycin drug selection marker into 177bp repeat region.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Insert

    162xLacO repeats derived from: PMID: 12864855.
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. LacZ-5BoxB in pcDNA3.1+

    Plasmid

    #29729

    Depositor

    Howard Chang

    Article

    Wang et al Nature. 2011 Mar 20. ():.

    Insert

    LacZ-5BoxB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only

32. pRAF-MutH

    Plasmid

    #86609

    Purpose

    E. coli MutH expression plasmid

    Depositor

    Richard Fishel

    Article

    Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

    Insert

    E. coli MutH
    

    Use

    Tags

    his-tag and ald-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRAF-MutS

    Plasmid

    #86605

    Purpose

    E. coli MutS expression plasmid

    Depositor

    Richard Fishel

    Article

    Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

    Insert

    E. coli MutS (D835R,R840E)
    

    Use

    Tags

    his-tag and ald-tag

    Expression

    Bacterial

    Mutation

    D835R, R840E

    Promoter

    T7

    Available since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. ptRNAfMet-A37

    Plasmid

    #196657

    Purpose

    Plasmid for overexpression of native initiator tRNA (A37)

    Depositor

    Markus Jeschek

    Article

    Hollerer et al Nucleic Acids Res. 2023 Mar 21;51(5):237

    Insert

    tRNAfMet-A37 expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    metY native promoter

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. DHFR-myc

    Plasmid

    #20214

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    DHFR (folA E. coli)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBRα

    Plasmid

    #53731

    Purpose

    Plpp/PlacUV5 -directed synthesis of E. coli RNAP alpha subunit; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.

    Depositor

    Ann Hochschild

    Article

    Dove et al Nature. 1997 Apr 10;386(6625):627-30.

    Insert

    αNTD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lpp/lacUV5 (tandem promoter)

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pFRET-HSP33 cys

    Plasmid

    #16076

    Depositor

    Ulrich Hammerling

    Article

    Guzy et al Cell Metab. 2005 Jun;1(6):401-8. doi: 10

    Insert

    HSP33 cys
    

    Use

    Tags

    ECFP and EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

38. pETSG

    Plasmid

    #159418

    Purpose

    Expression of proteins with a C-terminal thermostable GFP (TGP) tag in Escherichia coli

    Depositor

    Dianfan Li

    Article

    Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

    Insert

    thermostable GFP (TGP)
    

    Use

    Tags

    polyhistidine (poly-His)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET303-colE1TR

    Plasmid

    #155181

    Purpose

    Expresses colicin E1 residues 1-364

    Depositor

    Joanna Slusky

    Article

    Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

    Insert

    colicin E1 residues 1-364
    

    Use

    Tags

    C-terminal 6x Histidine tag

    Expression

    Bacterial

    Mutation

    truncation of colicin E1 including 1-364

    Promoter

    T7

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pTR47m4

    Plasmid

    #104068

    Purpose

    Luciferase Reporter for Formaldehyde Sensing

    Depositor

    David Liu

    Article

    Woolston et al Biotechnol Bioeng. 2017 Sep 16. doi: 10.

    Inserts

    xluxAB
    frmR
    frm Promoter m4
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    23r and 25th nucleotides in sequence mutated from…

    Promoter

    Available since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pBLind

    Plasmid

    #113109

    Purpose

    Vector encoding the RFP gene under blue light-inducible promoter BindF1.

    Depositor

    Chueh Loo Poh

    Article

    Jayaraman et al ACS Synth Biol. 2018 Apr 20;7(4):986-994

    Insert

    RFP
    

    Use

    Cell-free

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BindF1

    Available since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. zCD2

    Plasmid

    #122031

    Purpose

    Expresses histone deacetylase 6 (HDAC6) catalytic domain 2 (CD2), also known as zCD2, in Escherichia coli cells.

    Depositor

    David Christianson

    Article

    Hai et al Nat Chem Biol. 2016 Sep;12(9):741-7. doi

    Insert

    Zebrafish HDAC6 Catalytic Domain 2
    

    Use

    Tags

    His6-MBP tag with cleavable TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pDropVn

    Plasmid

    #184873

    Purpose

    Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for Vibrio natriegens genome editing protocol.

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    lacZalpha,ccdB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

44. pEF/myc/ER FLII12Pglu-700uDelta6

    Plasmid

    #17867

    Depositor

    Wolf Frommer

    Article

    Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

    Insert

    FLII12Pglu-700uDelta6
    

    Use

    Tags

    Citrine

    Expression

    Mammalian

    Mutation

    700uDelta6, ECFP insert in mglB

    Promoter

    Available since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pMVS1111A:PhmtB-bgaB

    Plasmid

    #205998

    Purpose

    Conjugative shuttle vector for Escherichia coli and Methanothermobacter thermautotrophicus encoding beta-galactosidase

    Depositor

    Bastian Molitor

    Article

    Fink et al mBio. 2021 Dec 21;12(6):e0276621. doi: 1

    Insert

    thermostable beta-galactosidase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    PhmtB

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. EcMBP165-cpGFP.PPYF.T203V.pRSET

    Plasmid

    #33373

    Purpose

    a single-wavelength maltose sensor constructed from E. coli MalE and cpGFP

    Depositor

    Loren Looger

    Article

    Marvin et al Proteins. 2011 Nov;79(11):3025-36. doi:

    Insert

    Improved MBP based Maltose Biosensor
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    cpGFP146 variant from GCaMP2 inserted between AA1…

    Promoter

    T7

    Available since

    Dec. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pNM3814

    Plasmid

    #154122

    Purpose

    mec-4 promoter driving tet ON rtetR-L-QFAD in an RCME vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Insert

    mec-4p::rtetR-L-QFAD::act-4 '3
    

    Use

    Rmce

    Tags

    Expression

    Worm

    Mutation

    Promoter

    not applicable

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pACYCDuet_WTDHFR_WTTS

    Plasmid

    #91226

    Purpose

    bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

    Depositor

    Kimberly Reynolds

    Article

    Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

    Inserts

    folA
    thyA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pKI1

    Plasmid

    #199118

    Purpose

    Expresses N-terminally Hisx6-tagged, SNAP-tagged full-length RapA in E. coli cells, suitable for fluorescent labelling

    Depositor

    Jeff Gelles

    Article

    Inlow et al Proc Natl Acad Sci U S A. 2023 Jul 11;12

    Insert

    H6-SNAP-RapA
    

    Use

    Tags

    6xHis and SNAP-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. EcMBP165-cpGFP.PPYF.pRSET

    Plasmid

    #33372

    Purpose

    a single-wavelength maltose sensor constructed from E. coli MalE and cpGFP

    Depositor

    Loren Looger

    Article

    Marvin et al Proteins. 2011 Nov;79(11):3025-36. doi:

    Insert

    MBP based Maltose Biosensor
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    cpGFP146 variant from GCaMP2 inserted between AA1…

    Promoter

    T7

    Available since

    Jan. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge