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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,383 results for: mpl

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Showing: 2731 - 2760 of 11383 results

  1. pD649-HAsp-SIGLEC10-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156906
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    SIGLEC10 (SIGLEC10 Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    Jan. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    156906-plasmid-map-sequence-id-302921

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  2. pcDNA5-FRT-TO-Nprot-HF_GC3opt

    Plasmid
    #157732
    Purpose
    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Article
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Insert
    Nprot-HF_GC3opt (N SARS-CoV-2)
    Tags
    His8-Flag
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Available Since
    Aug. 10, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157732-plasmid-map-sequence-id-308146

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  8. pODN-AAVS1-mNG-CAAX

    Plasmid
    #183870
    Purpose
    Repair template for the stable expression of a mNeonGreen-CAAX fusion in human cells using CRISPR/Cas9.
    Depositor
    Alisa Piekny
    Article
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Insert
    AAVS1 homology arms with CMV-driven mNeonGreen-CAAX fusion (AAVS1 Human)
    Use
    CRISPR; Donor template
    Tags
    mNeonGreen-CAAX
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Promoter
    CMV
    Available Since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    183870-plasmid-map-sequence-id-362419

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  10. pPB-tetO(hCMV1)-HA-Tet1(201R2)-IV

    Plasmid
    #102421
    Purpose
    Inducible expression of siRNA resistant mouse Tet1-201 (Ensembl transcript ENSMUST00000050826.13) with HA-tag and IRES-Venus in piggyBac (PB) transposon vector
    Depositor
    Kian Peng Koh
    Article
    Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi
    Insert
    Tet1 (Tet1 Mouse)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Modified at the Dharmacon SMARTpool siRNA #2 targ…
    Promoter
    TetO-CMV
    Available Since
    Nov. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    102421-plasmid-map-sequence-id-198790

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  16. pPB-tetO(hCMV1)-HA-Tet1mHxD(201R2)-IV

    Plasmid
    #102422
    Purpose
    Inducible expression of siRNA resistant mouse Tet1-201 (ENSMUST00000050826.13) with mutated catalytic domain (H1620Y & D1622A), HA-tag and IRES-Venus in piggyBac (PB) transposon vector
    Depositor
    Kian Peng Koh
    Article
    Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi
    Insert
    Tet1 catalytic domain mutant (Tet1 Mouse)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    H1620Y and D1622A mutations in Tet1 catalytic dom…
    Promoter
    TetO-CMV
    Available Since
    Nov. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    102422-plasmid-map-sequence-id-198789

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  17. HA-hDAGLa-V5

    Plasmid
    #87674
    Purpose
    Expresses human DAGLa protein with a HA tag inserted in the first extracellular loop, and intracellular V5 tag located on the C-terminal
    Depositor
    Pat Doherty
    Article
    Zhou et al Mol Cell Neurosci. 2016 Oct;76:76-86. do
    Insert
    Human Diacylglycerol Lipase Alpha (DAGLA Human)
    Tags
    HA and V5 tag
    Expression
    Mammalian
    Mutation
    Hemagglutinin (HA) peptide sequence (YPYDVPDYA) f…
    Promoter
    T7
    Available Since
    March 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    87674-plasmid-map-sequence-id-173603

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  19. pSems-leader-mXFPe-IFNGR2(30-337)

    Plasmid
    #192787
    Purpose
    Expression of mXFPe-tagged IFNGR2 at the plasma membrane, e.g. for single molecule fluorescence microscopy
    Depositor
    Jacob Piehler
    Article
    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165
    Insert
    IFNGR2 (IFNGR2 Human)
    Tags
    Ig k-chain leader sequence and mXFPe
    Expression
    Mammalian
    Mutation
    mXFPe: tyrosine 66 to phenylalanine, asparagine 1…
    Promoter
    CMV
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    192787-plasmid-map-sequence-id-379660

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  24. pST50Trc1-HISDHFR

    Plasmid
    #63935
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    63935-plasmid-map-sequence-id-177341

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  26. pBR HPV31 9E

    Plasmid
    #221009
    Purpose
    Human papillomavirus 31 isolate 9E, complete genome cloned in pBR322
    Depositor
    Alison McBride
    Insert
    Human papillomavirus type 31 genome 9E isolate
    Use
    Cloning vector
    Available Since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    221009-plasmid-map-sequence-id-436075

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Showing: 2731 - 2760 of 11383 results