Addgene

We narrowed to 11,819 results for: otos

Showing: 2731 - 2760 of 11819 results

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pLenti_CMV_pHoran4

Plasmid

#186975

Purpose

Expresses fluorescent pH sensor in mammalian cells

Depositor

Adam Cohen

Article

Hayward et al eLife. 2023 Oct 6;12:RP86833. doi: 10.75

Insert

pHoran4

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pJMP2822

Plasmid

#160670

Purpose

Mobile CRISPRi sfGFP 'test', empty sgRNA

Depositor

Jason Peters

Article

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Insert

empty sgRNA (BsaI)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pMOD3-mWasabi-nat

Plasmid

#120331

Purpose

Bacterial mWasabi promoter trap

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

mWasabi

Use

Tags

Expression

Bacterial

Mutation

Promoter

(None)

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG:Kaede

Plasmid

#39261

Depositor

Anna-Katerina Hadjantonakis

Article

Nowotschin et al BMC Dev Biol. 2009 Sep 9;9:49.

Insert

Kaede

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCAGGS

Available since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only
pTYF-G4BS-2TPH-EGFP-IRES-GAL4p65

Plasmid

#32569

Depositor

Sergey Kasparov

Article

Benzekhroufa et al Gene Ther. 2009 May;16(5):681-8. Epub 20

Inserts

EGFP
Gal4p65

Use

Lentiviral

Tags

Expression

Mutation

Promoter

2TPH

Available since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-Zeo-ecfp

Plasmid

#65038

Purpose

ZeoR mini-Tn7 vector, mobilizable, for CFP tagging bacteria

Depositor

Herbert Schweizer

Article

Choi et al Nat Protoc. 2006;1(1):153-61.

Insert

ecfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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YIplac211-KAR2-msGFP2-HDEL

Plasmid

#160468

Purpose

Yeast integration vector for the gene replacement of S. cerevisiae KAR2 fused to msGFP2 with an ER retrieval signal

Depositor

Benjamin Glick

Article

Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

Insert

KAR2-msGFP2-HDEL

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pMOD3-mCardinal-nat

Plasmid

#120333

Purpose

Bacterial mCardinal promoter trap

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

mCardinal

Use

Tags

Expression

Bacterial

Mutation

Promoter

(None)

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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YIPlac204TC-NLS-DsRed-Express2

Plasmid

#25443

Depositor

Benjamin Glick

Article

Strack et al Nat Methods. 2008 Nov . 5(11):955-7.

Insert

NLS-DsRed-Express2

Use

Tags

NLS

Expression

Yeast

Mutation

Promoter

Available since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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pACYC.GFP1Amb

Plasmid

#102792

Purpose

Expresses GFP with a premature stop codon

Depositor

Andrew Ellington

Article

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Insert

Green Fluorescent Protein

Use

Tags

Expression

Bacterial

Mutation

Y39Stop

Promoter

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCB1532H-RFP

Plasmid

#101855

Purpose

Golden-Gate compatible fungal transformation vector carrying Hygromycin Resistance with RFP screening cassette (RFP knocked out by GG reaction, allowing for red-white screening)

Depositor

Sophien Kamoun

Article

Pennington et al Figshare (2017)

Type

Empty backbone

Use

Fungal expression plasmid, tested in magnaporthe

Tags

Expression

Mutation

Promoter

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pRSET-B-frSkyran-S

Plasmid

#182209

Purpose

Bacterial expression of frSkylan-S

Depositor

Shiro Suetsugu

Article

Osuga et al Mol Biol Cell. 2021 Nov 1;32(21):br7. do

Insert

frSkylan-S

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR_TiTRE

Plasmid

#172128

Purpose

Entry vector for tightly controlled gene expression by a tetracycline-responsive promoter

Depositor

Kenji Kontani

Article

Araki et al J Biol Chem. 2021 Nov 18:101428. doi: 10

Type

Empty backbone

Use

Entry vector

Tags

Expression

Mammalian

Mutation

Promoter

tight TRE

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-Pcdh19-GFP

Plasmid

#35675

Depositor

James Jontes

Article

Biswas et al J Cell Biol. 2010 Nov 29;191(5):1029-41.

Insert

Pcdh19 (pcdh19 Zebrafish)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-dCry-V5His

Plasmid

#136241

Purpose

Protein expression of D. melanogaster (Dm) cytochrome with V5 and His tags

Depositor

Aziz Sancar

Article

Ozturk et al Biochemistry. 2009 Sep 15;48(36):8585-93

Insert

DmCry

Use

Tags

His and V5

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pBS-UPRT

Plasmid

#69310

Purpose

expresses UPRT cDNA

Depositor

Chris Doe

Article

Gay et al Nat Protoc. 2014 Feb;9(2):410-20. doi: 1

Insert

UPRT cDNA

Use

Tags

HA

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only
Ddo_PC2

Plasmid

#107629

Purpose

In situ hybridization and RNAi studies in planarians

Depositor

Alejandro Sánchez

Article

Accorsi et al The American Biology Teacher 79(3):208-2

Insert

PC2

Use

RNAi; In situ hybridization

Tags

Expression

Mutation

Promoter

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pJMP2824

Plasmid

#160671

Purpose

Mobile CRISPRi sfGFP 'test', empty sgRNA

Depositor

Jason Peters

Article

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Insert

empty sgRNA (BsaI)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pET29b-ClbP

Plasmid

#48244

Purpose

Untagged, full-length protein for in vivo peptidase assays

Depositor

Emily Balskus

Article

Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

Insert

ClbP

Use

Tags

None

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pBSKS Notch4

Plasmid

#21325

Depositor

Jan Kitajewski

Article

Uyttendaele et al Development. 1996 Jul . 122(7):2251-9.

Insert

Notch4 (Notch4 Mouse)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 11, 2009

Availability

Academic Institutions and Nonprofits only
pJMP2754

Plasmid

#160666

Purpose

Mobile CRISPRi sfGFP 'test', empty sgRNA

Depositor

Jason Peters

Article

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Insert

empty sgRNA (BsaI)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pEBB-TB

Plasmid

#36099

Depositor

Ezra Burstein

Article

Maine et al Nat Protoc. 2010 Aug;5(8):1447-59. Epub

Type

Empty backbone

Use

Tags

BT-tag

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 15, 2012

Availability

Academic Institutions and Nonprofits only

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pERB260

Plasmid

#67763

Purpose

Expresses BICD-mCherry-eDHFR

Depositor

Michael Lampson

Article

Ballister et al Curr Biol. 2015 May 18;25(10):R407-8. do

Insert

BICD-mCherry-eDHFR

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-intein/Src

Plasmid

#71406

Purpose

Expresses Src inserted with a Npu DnaE intein (F1 construct)

Depositor

Huiwang Ai

Article

Ren et al J Am Chem Soc. 2015 Feb 18;137(6):2155-8

Insert

Src active kinase domain inserted with Npu DnaE intein

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only
pKP660

Plasmid

#160812

Purpose

Expression of ExbB, ExbD proteins

Depositor

Kathleen Postle

Article

Ollis et al Mol Microbiol. 2009 Aug;73(3):466-81. do

Insert

exbB, exbD

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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sgPal1 (p2Tol-U6-sgPal1-HygR)

Plasmid

#71483

Purpose

Expresses palindromic sgRNA for use in self-cloning CRISPR

Depositor

Richard Sherwood

Article

Arbab et al Stem Cell Reports. 2015 Oct 27. pii: S22

Insert

sgPal1

Use

Tags

Expression

Mutation

Promoter

U6

Available since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pBA2SkIKmA2

Plasmid

#39214

Depositor

Anastasios Melis

Article

Lindberg et al Metab Eng. 2010 Jan;12(1):70-9. Epub 200

Insert

Synechocystis kudzu IspS

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

psbA2

Available since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only
Ddo_collagen

Plasmid

#107634

Purpose

In situ hybridization and RNAi studies in planarians

Depositor

Alejandro Sánchez

Article

Accorsi et al The American Biology Teacher 79(3):208-2

Insert

collagen

Use

RNAi; In situ hybridization

Tags

Expression

Mutation

Promoter

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-alpha2-chimaerin

Plasmid

#59316

Purpose

Expression plasmid for GFP-tagged alpha2-chimaerin (GFP at N-terminus of alpha2-chimaerin)

Depositor

Peter Scheiffele

Article

Beg et al Neuron. 2007 Sep 6;55(5):768-78.

Insert

alpha2-chimaerin

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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234_pSKB3-Venus-LUMP

Plasmid

#65975

Purpose

Bacterial expression of Venus-LUMP FRET pair

Depositor

Gerard Marriott

Article

Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. p

Insert

LUMP

Use

Tags

Venus

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge