We narrowed to 15,949 results for: LEA
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Plasmid#176202PurposeSingle INBox (INCENP 819-918) recruitment responsible for targeted Aurora B activation and recruitment using TMP-Halo dimerization systemDepositorInsertmCherry-INBox-eDHFR
UseTagsExpressionMammalianMutationPromoterAvailable SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAG.mCherry-INBox(AAA)-eDHFR
Plasmid#176207PurposeINBox TSS(892-894)-mutant replaced by AAA responsible for targeted Aurora B recruitment without activation using TMP-Halo dimerization systemDepositorInsertmCherry-INBoxAAA-eDHFR
UseTagsExpressionMammalianMutationPromoterAvailable SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
TetO::EGFP-hMeikin(8A mut)
Plasmid#174707PurposeDoxycycline inducible expression of human Meikin with 8 alanine mutations that eliminate Plk1 bindingDepositorInsertMeikin
UseTagsEGFPExpressionMammalianMutationS175A, T176A, T180A, S181A, S196A, T251A, T264A, …PromoterAvailable SinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH775_pJH_LldR_PlldR-sfGFP.2opal
Plasmid#163734PurposeLactate-inducible expression of green fluorescent protein encoding two opal codonsDepositorInsertlldr gfp.2TGA
UseTagsExpressionBacterialMutationgfp with first two leucine codons mutated to amberPromoterAvailable SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-PGRMC1-1-54
Plasmid#173472PurposePGRMC1 lacking the C-terminal region downstream of the transmembrane domainDepositorInsertPGRMC1 (PGRMC1 Human)
UseTagsHAExpressionMammalianMutationDeleted for amino acids 55-194PromoterAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-PGRMC1-21-195
Plasmid#173473PurposePGRMC1 lacking the N-terminal region upstream of the transmembrane regionDepositorInsertPGRMC1 (PGRMC1 Human)
UseTagsHAExpressionMammalianMutationDeleted for amino acids 1-20PromoterAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc120
Plasmid#161950PurposeContains elements for building reporter plasmid for IBM. Built upon pSB4A3. ECF20 reporter plasmidDepositorInsertpSB4A3-PECF20-B30-mScarlet-I-B15
UseTagsExpressionBacterialMutationPromoterPECF20Available SinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc393
Plasmid#161953PurposeDemonstrates chemically induced dimmerization of acVHH. T7 RNAP polymerase reporter plasmidDepositorInsertpSB4A3-T-PT7-B30-mScarlet-I-3T
UseTagsExpressionBacterialMutationPromoterPT7Available SinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc330
Plasmid#161952PurposeContains elements for building reporter plasmid for IBM. SrpR reporter plasmidDepositorInsertpSEVA171-PSrpR-B30-mScarlet-I-Ter
UseTagsExpressionBacterialMutationPromoterPSrpRAvailable SinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc050
Plasmid#161938PurposeCarries Golden Gate substitution insert for IBM with M86 intein. Plux2 as C-lobe promoterDepositorInsertpSB1A3-BbsI-M86N-cat-Plux2-B32-M86C-SapI
UseTagsExpressionBacterialMutationPromoterPlux2Available SinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc259
Plasmid#161951PurposeContains elements for building reporter plasmid for IBM.TetR reporter plasmidDepositorInsertpSEVA471-PTet*-RiboJ10-B64-mScarlet-I-Ter
UseTagsExpressionBacterialMutationPromoterPTet*Available SinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nup54 no FG-mEGFP494(2)
Plasmid#164271PurposeMammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 7 amino acids at the carboxyl end of mEGFPDepositorInsertNup54 (NUP54 Human)
UseTagsmEGFPExpressionMammalianMutationdeleted the FG region of Nup54PromoterCMVAvailable SinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nup54 no FG-mEGFP494(1)
Plasmid#164270PurposeMammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 6 amino acids at the carboxyl end of mEGFPDepositorInsertNup54 (NUP54 Human)
UseTagsmEGFPExpressionMammalianMutationdeleted the FG region of Nup54PromoterCMVAvailable SinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCbh-SpyCas9-2A-puro-pA_U6-sgRosa26
Plasmid#149346PurposeMammalian expression vector for SpyCas9, puromycin resistance and mouse Rosa26 guide RNADepositorInsertCas9
UseCRISPRTagsFlag tagExpressionMammalianMutationPromoterCbcAvailable SinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCbh-SpyCas9-2A-puro-pA_U6-BbsI
Plasmid#149347PurposeMammalian expression vector for SpyCas9 and puromycin resistanceDepositorInsertSpyCas9
UseCRISPRTagsFlag tagExpressionMammalianMutationPromoterCbhAvailable SinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRII-TOPO CMV-cGFP-SV40 pA + Laccase2 Exon 2
Plasmid#91802PurposeExpresses the Laccase2 circular RNA when the upstream SV40 poly(A) signal fails to be usedDepositorInsertCoral Green Fluorescent Protein (cGFP)
UseTagsExpressionMammalianMutationPromoterCMVAvailable SinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEM_HcBOP1ainsitu
Plasmid#136529PurposeTemplate for generating in situ hybridization probeDepositorInsertHouttuynia cordata BLADE-ON-PETIOLE homolog1a
UseCloning vectorTagsExpressionMutationPromoterAvailable SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEM_ZmaBOP1binsitu
Plasmid#136531PurposeTemplate for generating in situ hybridization probeDepositorInsertZoysia matrella BLADE-ON-PETIOLE homolog1b
UseCloning vectorTagsExpressionMutationPromoterAvailable SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEM_ZmaBOP2binsitu
Plasmid#136532PurposeTemplate for generating in situ hybridization probeDepositorInsertZoysia matrella BLADE-ON-PETIOLE homolog2b
UseCloning vectorTagsExpressionMutationPromoterAvailable SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEM_HcBOP1binsitu
Plasmid#136530PurposeTemplate for generating in situ hybridization probeDepositorInsertHouttuynia cordata BLADE-ON-PETIOLE homolog1b
UseCloning vectorTagsExpressionMutationPromoterAvailable SinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pst39-NST-clone14
Plasmid#133421PurposeS. cerevisiae Nip1 (with C-terminal TEV cleavage site and strep-II tag), Sui1 and Tif5B6 (with N-terminal 7xHis tag and TEV cleavage set) coding sequences with intervening ribosome binding sites and other cis-elements for expression as a polycistron, T7 theta 10 promoter (and corresponding terminator after polycistron).DepositorUseTagsExpressionBacterialMutationORF for Nip1 encodes aa 4-156 (not complete ORF),…PromoterAvailable SinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK3_031
Plasmid#123745PurposeEncodes p38 Translocation Reporter (p38 KTR) fused to mCerulean3 as a Type 3 part to be used in the MTK systemDepositorInsertp38KTR-mCerulean3
UseTagsExpressionMammalianMutationPromoterAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY86
Plasmid#130949PurposeEngineered sgRNA-LEA2-WTmis2 generator circuit including promoter Plux2, 2 BoxB aptamers and 2 mis-matches in sgRNA scaffoldDepositorInsertsgRNA-LEA2-WTmis2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPlux2Available SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMtnA UTR delta 21-55 eGFP SV40
Plasmid#132653PurposeExpresses an eGFP mRNA from the Drosophila Metallothionein A promoter that has 20 nt of the MtnA 5' UTRDepositorInserteGFP
UseTagsExpressionInsectMutationPromoterMtnAAvailable SinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMtnA UTR delta 41-55 eGFP SV40
Plasmid#132652PurposeExpresses an eGFP mRNA from the Drosophila Metallothionein A promoter that has 40 nt of the MtnA 5' UTRDepositorInserteGFP
UseTagsExpressionInsectMutationPromoterMtnAAvailable SinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0048
Plasmid#117611PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for pEPOR1CB0012(pEPOR1CB0012) and sgRNA_Rpn2/Psmd1 subunit {AT2G32730}(pEPOR1CB0075)DepositorInsert[35S:pEPOR1CB0012(pEPOR1CB0012) ] +[AtU6-26:sgRNA]
UseTagsExpressionPlantMutationPromoterAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH90
Plasmid#128203PurposecrRNA backbone for FnCas12a/CmsI nucleases, combine with TaU3 promoter module (pFH33)DepositorInsertcrRNA backbone for FnCas12a/CmsI nucleases, combine with TaU3 promoter module (pFH33)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH104
Plasmid#128209PurposecrRNA backbone for FnCas12a/CmsI nucleases, combine with AtU6-26 promoter module (pFH35)DepositorInsertcrRNA backbone for FnCas12a/CmsI nucleases, combine with AtU6-26 promoter module (pFH35)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTEI126
Plasmid#126066PurposeModule 1 vector of PlaMiNGo toolkit for expression of plastid targeted GFP1-10 in plant cellDepositorInsertChloroplastic FNR transit peptide
UseBinaryTagsGFP1-10ExpressionPlantMutationPromoter'long' CaMV35SAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTEI127
Plasmid#126067PurposeModule 1 vector of PlaMiNGo toolkit for expression of mitochondria targeted GFP1-10 in plant cellDepositorInsertmitochondria Rieske iron sulphur protein (1-100 aa)
UseBinaryTagsGFP1-10ExpressionPlantMutationPromoter'long' CaMV35SAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTEI177
Plasmid#126077PurposeModule 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP1-10 and expression in plant cellDepositorInsertsccdB Cassette (BsaI)
GFP1-10
UseBinary, module 1Tagslinker (GTGGGGS)ExpressionPlantMutationPromoterAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTEI129
Plasmid#126210PurposeModule 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11x7 and expression in plant cellDepositorInsertsccdB Cassette (BsaI)
GFP11x7 (5 aa linker)
UseBinary, module 1TagsExpressionPlantMutationPromoterAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTEI161
Plasmid#126211PurposeModule 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11 and expression in plant cellDepositorInsertsccdB Cassette (BsaI)
GFP11
UseBinary, module 1TagsExpressionPlantMutationPromoterAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJPL3[flp-6p::cmk-1::GFP]
Plasmid#124613PurposeUsed to confirm the expression of the trans-spliced GFP reporter in the expected ASE neuronsDepositorInsert[flp-6p::cmk-1::GFP]
UseTagsExpressionWormMutationPromoterAvailable SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0055
Plasmid#117622PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_Methyltransferase {AT5G10620}(pEPOR1CB0083)DepositorInsert[35S:Sp-eCas9 1.1(pEPOR1CB0011) ] +[AtU6-26:sgRNA]
UseTagsExpressionPlantMutationPromoterAvailable SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
EGFP-miSFIT-12-C
Plasmid#124693PurposeTuning expression of EGFPDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterminimal CMVAvailable SinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
EGFP-miSFIT-9-G
Plasmid#124692PurposeTuning expression of EGFPDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterminimal CMVAvailable SinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
EGFP-miSFIT-6-C,16-C
Plasmid#124694PurposeTuning expression of EGFPDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterminimal CMVAvailable SinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
EGFP-miSFIT-10-G, 15-C
Plasmid#124695PurposeTuning expression of EGFPDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterminimal CMVAvailable SinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0023
Plasmid#117538PurposeLevel1 Golden Gate Cassette: extended stem sgRNA cassette with AtU6-26 terminator for SpCas9, targeting NbPDS gene in N. benthamianaDepositorInsertsgRNA (Sp Cas9) - extended stem with U626 terminator
UseTagsExpressionPlantMutationPromoterAvailable SinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0080
Plasmid#117647PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for eSaCas9(pEPOR1CB0113) and sgRNA_ENTH/ANT {AT1G14910}(pEPOR1CB0100)and sgRNA_ENTH/ANT {AT2G01600}(pEPOR1CB0111)DepositorInsert[35S:eSaCas9(pEPOR1CB0113) ] +[AtU6-26:sgRNA]
UseTagsExpressionPlantMutationPromoterAvailable SinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0076
Plasmid#117643PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for eSaCas9 (pEPOR1CB0113), sgRNA {AT1G62600}(pEPOR1CB0098) and sgRNA PPR-Adenylate Cyclase {AT1G62590}(pEPOR1CB0109)DepositorInsert[35S:eSaCas9(pEPOR1CB0113) ] +[AtU6-26:sgRNA]
UseTagsExpressionPlantMutationPromoterAvailable SinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0074
Plasmid#117641PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for Sp-xCas9 3.7(pEPOR1CB0012) and sgRNA_AtIPCS1 {AT3G54020}(pEPOR1CB0093)and sgRNA_AtIPCS1 {AT3G54020}(pEPOR1CB0104)DepositorInsert[35S:Sp-xCas9 3.7(pEPOR1CB0012) ] +[AtU6-26:sgRNA]
UseTagsExpressionPlantMutationPromoterAvailable SinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0073
Plasmid#117640PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_AtIPCS1 {AT3G54020}(pEPOR1CB0093)and sgRNA_AtIPCS1 {AT3G54020}(pEPOR1CB0104)DepositorInsert[35S:Sp-eCas9 1.1(pEPOR1CB0011) ] +[AtU6-26:sgRNA]
UseTagsExpressionPlantMutationPromoterAvailable SinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0072
Plasmid#117639PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.0(pEPOR1CB0010) and sgRNA_AtIPCS1 {AT3G54020}(pEPOR1CB0093)and sgRNA_AtIPCS1 {AT3G54020}(pEPOR1CB0104)DepositorInsert[35S:Sp-eCas9 1.0(pEPOR1CB0010) ] +[AtU6-26:sgRNA]
UseTagsExpressionPlantMutationPromoterAvailable SinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0071
Plasmid#117638PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_AtIPCS1 {AT3G54020}(pEPOR1CB0093)and sgRNA_AtIPCS1 {AT3G54020}(pEPOR1CB0104)DepositorInsert[35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]
UseTagsExpressionPlantMutationPromoterAvailable SinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0070
Plasmid#117637PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for Sp-xCas9 3.7(pEPOR1CB0012) and sgRNA_AtCas6 {AT5G04770}(pEPOR1CB0092)and sgRNA_AtCas6 {AT5G04770}(pEPOR1CB0103)DepositorInsert[35S:Sp-xCas9 3.7(pEPOR1CB0012) ] +[AtU6-26:sgRNA]
UseTagsExpressionPlantMutationPromoterAvailable SinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0069
Plasmid#117636PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_AtCas6 {AT5G04770}(pEPOR1CB0092)and sgRNA_AtCas6 {AT5G04770}(pEPOR1CB0103)DepositorInsert[35S:Sp-eCas9 1.1(pEPOR1CB0011) ] +[AtU6-26:sgRNA]
UseTagsExpressionPlantMutationPromoterAvailable SinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0068
Plasmid#117635PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.0(pEPOR1CB0010) and sgRNA_AtCas6 {AT5G04770}(pEPOR1CB0092)and sgRNA_AtCas6 {AT5G04770}(pEPOR1CB0103)DepositorInsert[35S:Sp-eCas9 1.0(pEPOR1CB0010) ] +[AtU6-26:sgRNA]
UseTagsExpressionPlantMutationPromoterAvailable SinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0067
Plasmid#117634PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_AtCas6 {AT5G04770}(pEPOR1CB0092)and sgRNA_AtCas6 {AT5G04770}(pEPOR1CB0103)DepositorInsert[35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]
UseTagsExpressionPlantMutationPromoterAvailable SinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only