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Plasmid#100167PurposeUsed to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growthDepositorInsertMevT and MBIS operon
UseTagsExpressionBacterialMutationPromoterlacUV5Available sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mCherry-FKBP-GEF(TIAM1)
Plasmid#85156Purposeacute activation of Rac through rapamycin-induced translocation to membrane-localized FRBDepositorInsertFusion of mCherry, FKBP, and the DH-domain of human TIAM1 (amino acids 1012-1250)
UseTagsmCherry-FKBPExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pST_AfrLEA3m (pBS0911)
Plasmid#185285PurposeFor the mammalian expression of the brine shrimp protein AfrLEA3m. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertAfrLEA3m
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pST_PvLEA4_repeats_k3_26 (pBS0762)
Plasmid#185244PurposeFor the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k3_26. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertPvLEA4_repeats_k3_26
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pST_SERF2_HUMAN (pBS0751)
Plasmid#185239PurposeFor the mammalian expression of the human protein SERF2_HUMAN. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertSERF2_HUMAN
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pST_MAHS_RAMVA (pBS0310)
Plasmid#185169PurposeFor the mammalian expression of the tardigrade protein MAHS_RAMVA. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertMAHS_RAMVA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
Keasling-2337
Plasmid#100168PurposeUsed to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growthDepositorInsertlacIq-Ptrc-ADS
UseTagsExpressionBacterialMutationPromotertrcAvailable sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pST_DHN_AtCor47 (pBS0784)
Plasmid#185254PurposeFor the mammalian expression of the Arabidopsis protein DHN_AtCor47. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).DepositorInsertDHN_AtCor47
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
FKBP-YPet-RanGAP*
Plasmid#175245PurposeBacterial expression and purification, SUMOylation substrate that can be recruited to FRB with rapamycin, Ypet is a FRET acceptor for CyPetDepositorInsertRANGAP1 (RANGAP1 Human)
UseTagsExpressionBacterialMutationAmino acid 562 F to APromotertacAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
alpha1Ddelta-G12-FRB
Plasmid#87454Purposerapamycin-mediated heterodimerization between IQV and PCRD/DCRD from C-terminal of LTCCsDepositorInserta1D short variants (Cacna1d Rat)
UseTagsFRBExpressionMammalianMutationdelete KYPAKNTTIALQMLERMLPromoterCMVAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
L2111-FKBP-GPA-20-11S
Plasmid#222882PurposeBiosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.DepositorInsertFKBP-GPA-20GS-NanoLuc11S
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2375-FKBP-GPA-20-11S
Plasmid#222897PurposeBiosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.DepositorInsertFKBP-GPA-20GS-NanoLuc11S
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2376-FKBP-GPA-20-114
Plasmid#222896PurposeBiosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114.DepositorInsertFKBP-GPA-20GS-NanoLuc11S
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2374-FRB-GPA-20-11S
Plasmid#222895PurposeBiosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.DepositorInsertFRB-GPA-20GS-NanoLuc11S
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2373-FRB-GPA-20-114
Plasmid#222894PurposeBiosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114.DepositorInsertFRB-GPA-20GS-NanoLuc114
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2179-FRB-GPA-6-GFPS
Plasmid#222893PurposeBiosensor chain detecting rapamycin and responding with split GFP reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 6 GS linker, split GFP fragment small.DepositorInsertFRB-GPA-6GS-GFPS
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2110-FKBP-GPA-20-114
Plasmid#222881PurposeBiosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114.DepositorInsertFKBP-GPA-20GS-NanoLuc114
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2108-FRB-GPA-20-114
Plasmid#222879PurposeBiosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114.DepositorInsertFRB-GPA-20GS-NanoLuc114
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2109-FRB-GPA-20-11S
Plasmid#222880PurposeBiosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.DepositorInsertFRB-GPA-20GS-NanoLuc11S
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
LBR(R583Q)-FKBP-GFP
Plasmid#222423PurposeTo express a cholesterol synthesis point mutant of LBR (R583Q) with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cellsDepositorInsertLBR(R583Q) (LBR Human)
UseTagsFKBP-GFPExpressionMammalianMutationR583QPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only