We narrowed to 6,648 results for: tac

1. Dom neg CstF64

   Plasmid

   #127250

   Purpose

   Expresses the Dominant Negative CstF64 in mammalian cells; DN blocks polyadenylation

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   CstF64 delta 282 (CSTF2 Human)
   

   Use

   Other

   Tags

   Expression

   Mammalian

   Mutation

   insert @SanD1:GTCCAGGCGCCTACCCATACGACGTCCCAGACTAC…

   Promoter

   pEF1a

   Available since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOTTC946 - pAAV CMV-IE NK1R-IRES-EGFP

   Plasmid

   #102935

   Purpose

   An AAV vector that expresses rat NK1R-IRES-EGFP under the CMV-IE promoter

   Depositor

   Brandon Harvey

   Article

   Nelson et al Neuroscience. 2019 Jun 23;413:77-85. doi

   Insert

   NK1R (Tacr1 Rat)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-IE

   Available since

   Sept. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLC-RFP657-CASP8

   Plasmid

   #75164

   Purpose

   LentiCRISPR-RFP657 with sgRNA targeting human Caspase-8

   Depositor

   Beat Bornhauser

   Article

   McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

   Insert

   CASP8 sgRNA (CASP8 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUDE710

   Plasmid

   #103020

   Purpose

   expression of a Cpf1 programming crRNA targeting ADE2 and HIS4 (crADE2-3 crHIS4-4.S)

   Depositor

   Jean-Marc Daran

   Article

   Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

   Insert

   crADE2-3.crHIS4-4.S (ADE2, HIS4 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52

   Available since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAVS1-A2UCOE-EF1a-iCasp9-P2A-HLA-E SCT-T2A-hCD55-E2A-neo-AAVS1

   Plasmid

   #205444

   Purpose

   human AAVS1-targeting plasmid for expression of human CD46, HLA-G SCT, CD47, and CD59

   Depositor

   Deepta Bhattacharya

   Article

   Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

   Insert

   iCasp9-HLA-E SCT-hCD55
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLAP-BUBR1-dKARD

   Plasmid

   #114053

   Purpose

   transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

   Depositor

   Geert Kops

   Article

   Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

   Insert

   BUBR1-dKARD (BUB1B Human)
   

   Use

   Tags

   YFP-TEV-S- Human LAP

   Expression

   Mammalian

   Mutation

   C2823A and G2826A (siRNA-resistance): AGATACTAGCT…

   Promoter

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLCHKO_SFRS7_exon_deletion_3

   Plasmid

   #155071

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

   Depositor

   Jason Moffat, Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLCHKO_MDM4_exon_deletion_3

   Plasmid

   #155075

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

   Depositor

   Jason Moffat, Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cntn6-AP-His

   Plasmid

   #71944

   Purpose

   Expresses the extracellular region of the Contactin 6 protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Cntn6 (Cntn6 Mouse)
   

   Use

   Tags

   AP-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. ROR2 gRNA (BRDN0001146490)

    Plasmid

    #77940

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ROR2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ROR2 (ROR2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pk335-CARGO-12mer-14kb-USF-1-12

    Plasmid

    #227472

    Purpose

    12-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    CARGO to 14kb Upstream Fgf5
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmiRGLO-Zc3h12a 3’UTR

    Plasmid

    #222662

    Purpose

    Luciferase reporter vector containing mouse Zc3h12a 3'UTR

    Depositor

    Silvia Monticelli

    Article

    Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

    Insert

    Zc3h12a 3'UTR (Zc3h12a Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA3-FLAG-Regnase-3_1-547 aa

    Plasmid

    #222657

    Purpose

    Mammalian expression vector to express 1-547 aa of Regnase-3 tagged with FLAG at N-term

    Depositor

    Silvia Monticelli

    Article

    Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

    Insert

    Zc3h12c 1-547 aa (Zc3h12c Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    mutated histidine 548 to a stop codon

    Promoter

    CMV

    Available since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA3-FLAG-Regnase-3_1-409 aa

    Plasmid

    #222658

    Purpose

    Mammalian expression vector to express 1-409 aa of Regnase-3 tagged with FLAG at N-term

    Depositor

    Silvia Monticelli

    Article

    Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

    Insert

    Zc3h12c 1-409 aa (Zc3h12c Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    mutated proline 410 to a stop codon

    Promoter

    CMV

    Available since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA3-FLAG-Regnase-3_1-244 aa

    Plasmid

    #222659

    Purpose

    Mammalian expression vector to express 1-244 aa of Regnase-3 tagged with FLAG at N-term

    Depositor

    Silvia Monticelli

    Article

    Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

    Insert

    Zc3h12c 1-244 aa (Zc3h12c Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    mutated asparagine 245 to a stop codon

    Promoter

    CMV

    Available since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDH-Regnase-3 WT-T2A-copGFP

    Plasmid

    #222660

    Purpose

    Lentiviral vector to express mouse Regnase-3 WT

    Depositor

    Silvia Monticelli

    Article

    Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

    Insert

    Zc3h12c (Zc3h12c Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    mutated stop codon prior to a T2A peptide

    Promoter

    EF1α

    Available since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA3-Regnase-1 D141N

    Plasmid

    #222654

    Purpose

    Mammalian expression vector to express mouse Regnase-1 D141N mutant

    Depositor

    Silvia Monticelli

    Article

    Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

    Insert

    Zc3h12a (Zc3h12a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mutated aspartic acid 141 to asparagine to abroga…

    Promoter

    CMV

    Available since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC57 mini-FLAG-HA-Regnase-3 D252N

    Plasmid

    #222652

    Purpose

    For in vitro transcription of mouse Zc3h12c D252N mutant tagged with FLAG-HA at N-term

    Depositor

    Silvia Monticelli

    Article

    Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

    Insert

    Zc3h12c (Zc3h12c Mouse)
    

    Use

    In vitro transcription

    Tags

    FLAG and HA

    Expression

    Mutation

    mutated aspartic acid 252 to asparagine to abroga…

    Promoter

    T7

    Available since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC57 mini-ZsGreen-P2A-FLAG-HA-Regnase-1 WT

    Plasmid

    #222649

    Purpose

    For in vitro transcription of mouse Zc3h12a tagged with FLAG-HA at N-term

    Depositor

    Silvia Monticelli

    Article

    Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

    Insert

    Zc3h12a (Zc3h12a Mouse)
    

    Use

    In vitro transcription

    Tags

    FLAG and HA

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLCHKO_SFRS7_exon_deletion_4

    Plasmid

    #155072

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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