We narrowed to 24,396 results for: p

1. pdCas9-DNMT3A-PuroR_hNT

   Plasmid

   #71830

   Purpose

   Expression of dCas9-DNMT3A fusion with T2A-PuroR and non-targeting control sgRNA for use in human cells

   Depositor

   Vlatka Zoldos

   Article

   Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

   Insert

   Non-targeting sgRNA human (DNMT3A Synthetic, Human, S. pyogenes)
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, and T2A-PuroR

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9

   Promoter

   U6

   Available since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSPL3-ABCC8-2

   Plasmid

   #135916

   Purpose

   Encodes ABCC8 exon 2 wild type for the analysis of splicing variants

   Depositor

   Diana Valverde Pérez

   Article

   Lago-Docampo et al Sci Rep. 2020 Sep 15;10(1):15135. doi: 1

   Insert

   ATP-binding Cassette subfamily C member 8 (ABCC8 Human)
   

   Use

   Exon trapping

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3. Flag-CSL R192H

   Plasmid

   #167005

   Purpose

   Mammalian expression of CSL R192H

   Depositor

   Gian-Paolo Dotto

   Article

   Bottoni et al Nat Commun. 2019 Aug 29;10(1):3884. doi:

   Insert

   CSL (RBPJ Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   R192H

   Promoter

   CMV

   Available since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. FRB-TagRFPT-CAAX

   Plasmid

   #87710

   Purpose

   FRB-tagged TagRFPT; targeted to plasma membrane.

   Depositor

   Jin Zhang

   Article

   Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

   Insert

   FRB-TagRFPT-CAAX
   

   Use

   Tags

   6xHis, C-terminal targeting sequence from Kras, T…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XOllas

   Plasmid

   #217512

   Purpose

   KD Recombinase dependent conditional smGdP-10XOllas cassette

   Depositor

   S. Lawrence Zipursky

   Article

   Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

   Insert

   KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XOllas
   

   Use

   Tags

   smGdP-10XOllas

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Cav2.1 HA pcDNA3

   Plasmid

   #206069

   Purpose

   expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and an exofacial double HA tag in domain II

   Depositor

   Annette Dolphin

   Article

   Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

   Insert

   cacna1a (Cacna1a Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-HNF1A-WT-Flag-UTR

   Plasmid

   #183237

   Purpose

   Expression of FLAG tagged HNF1A

   Depositor

   Jorge Ferrer

   Article

   Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

   Insert

   HNF1 homeobox A (HNF1A Human)
   

   Use

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSPL3-ABCC8-9-10

   Plasmid

   #135918

   Purpose

   Encodes ABCC8 exons 9 and 10 wild type for the analysis of splicing variants

   Depositor

   Diana Valverde Pérez

   Article

   Lago-Docampo et al Sci Rep. 2020 Sep 15;10(1):15135. doi: 1

   Insert

   ATP-binding Cassette subfamily C member 8 (ABCC8 Human)
   

   Use

   Exon trapping

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAV-nEF-dCas9

   Plasmid

   #106430

   Purpose

   Expresses SpdCas9 from the nEF promoter in AAV backbone

   Depositor

   Juan Belmonte

   Article

   Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

   Insert

   nEF-dCas9
   

   Use

   AAV and CRISPR

   Tags

   HA-NLS and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   nEF

   Available since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. Donor_ANKRD1_KO_Puro

    Plasmid

    #186667

    Purpose

    Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, puromycin and polyA sequence is inserted between two homology arms.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    ANKRD1 KO Puromycin (ANKRD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pdCas9-DNMT3A-PuroR_BACH2-sgRNA8

    Plasmid

    #71828

    Purpose

    Expression of dCas9-DNMT3A fusion with T2A-PuroR and specific sgRNA for targeted DNA methylation of BACH2 promoter in human cells; for use as a control

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    BACH2-sgRNA8 (BACH2 Synthetic, Human, S. pyogenes)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9

    Promoter

    U6

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGL3-Basic-Rab18-sfGFP(N) HDR template

    Plasmid

    #129415

    Purpose

    HDR tempalte for tagging of endogenous human RAB18 N-terminus with sfGFP

    Depositor

    Elina Ikonen

    Article

    Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

    Insert

    RAB18 HDR template (RAB18 Human)
    

    Use

    CRISPR and TALEN; Endogenous tagging hdr template

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Donor_YTHDF2_KO_Hygro

    Plasmid

    #186671

    Purpose

    Donor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Hygromycin and polyA sequence is inserted between two homology arms.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    YTHDF2 KO Hygromycin (YTHDF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Donor_YTHDF2_KO_Puro

    Plasmid

    #186670

    Purpose

    Donor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Puromycin and polyA sequence is inserted between two homology arms.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    YTHDF2 KO Puromycin (YTHDF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FKBP-Dronpa

    Plasmid

    #87709

    Purpose

    FKBP-tagged Dronpa.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

    Insert

    FKBP-Dronpa
    

    Use

    Tags

    6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPbcas13b

    Plasmid

    #89906

    Purpose

    Bacterial expression for pbCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.

    Depositor

    Feng Zhang

    Article

    Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

    Insert

    Cas13b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FLAG-EglN2-pLenti6

    Plasmid

    #36950

    Depositor

    William Kaelin

    Article

    Koivunen et al Nature. 2012 Feb 15;483(7390):484-8. doi

    Insert

    EglN2 (EGLN2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Wildtype

    Promoter

    CMV

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Ef1a-DIO-BiPOLES-mCerulean

    Plasmid

    #154949

    Purpose

    Optogenetic tool for blue-light inhibition and red-light excitation of neurons

    Depositor

    Simon Wiegert

    Article

    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

    Insert

    BiPOLES
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1-alpha

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FLAG-EglN3-pLenti6

    Plasmid

    #36951

    Depositor

    William Kaelin

    Article

    Koivunen et al Nature. 2012 Feb 15;483(7390):484-8. doi

    Insert

    EglN3 (EGLN3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Wildtype

    Promoter

    CMV

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XHA

    Plasmid

    #217511

    Purpose

    KD Recombinase dependent conditional smGdP-10XHA cassette

    Depositor

    S. Lawrence Zipursky

    Article

    Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

    Insert

    KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XHA
    

    Use

    Tags

    smGdP-10XHA

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge