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We narrowed to 8,813 results for: Ott

Showing: 4651 - 4700 of 8813 results

  8. pILGFPEasy9R

    Plasmid
    #218291
    Purpose
    Plasmid-free in-situ promoter cloning and characterisation system in S. cerevisiae, allows the characterisation of a bidirectional promoter using yEGFP and E2-Crimson as reporters.
    Depositor
    Claudia Vickers
    Article
    McDonnell et al bioRxiv 2024.03.26.586389
    Insert
    pURA3>KlURA3>tKlURA3-tPGK1KanMX>tAgTEF-pMAL32>MazF(RPL28i)-E2Crimson>tPDC1-pDDI2>ISceI>tSynth3>ura3(4,191)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218291-plasmid-map-sequence-id-433736

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  9. pToxAmp21R

    Plasmid
    #218287
    Purpose
    A complete plasmid for ToxAmp (Toxin-antitoxin-driven gene amplification) for the expression of AeBlue
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    HO(-955, -789)-LoxP>pKlLEU2>KlLEU2>PCRT1>RelB>tPDC1-pTDH3>AeBlue>tSYNth7-ARS712-HO(-731, -264)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218287-plasmid-map-sequence-id-433689

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  10. pToxAmp12

    Plasmid
    #218286
    Purpose
    Multi-copy integration of heterologous genes (AeBlue and RelB) through co-transformation with ToxAmp (toxin-antitoxin-driven gene amplification) modules
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    HO(-253, -1)-pCRT1>RelB>tPDC1-pTDH3>AeBlue>tsynth7-ARS712-HO(-731, -264)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218286-plasmid-map-sequence-id-433688

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  11. pToxAmp1

    Plasmid
    #218285
    Purpose
    Amplifying the gene copy number of heterologous genes (yEGFP and RelB) through ToxAmp (toxin-antitoxin-driven gene amplification) mechanism
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    HO(-253, -1)-pRPL8B>RelB>tPDC1-pTEF1>yEGFP>tURA3-ARS712-HO(-731, -264)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218285-plasmid-map-sequence-id-433687

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  14. pJCC_162 SpCas9 KES(1107-1109)GG

    Plasmid
    #179526
    Purpose
    For bacterial expression of SpCas9 KES(1107-1109)GG (phosphate lock loop mutant) with an N-terminal His-MBP tag
    Depositor
    Jennifer Doudna
    Article
    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-
    Insert
    SpCas9 KES(1107-1109)GG
    Use
    Tags
    10X His, TEV protease cleavage site, and maltose-…
    Expression
    Bacterial
    Mutation
    replaced KES (residues 1107-1109) with GG
    Promoter
    Available since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    179526-plasmid-map-sequence-id-351748

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  15. pJCC_164 SpCas9 R1333A/R1335A

    Plasmid
    #179528
    Purpose
    For bacterial expression of SpCas9 R1333A/R1335A (PAM-binding arginines mutant) with an N-terminal His-MBP tag
    Depositor
    Jennifer Doudna
    Article
    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-
    Insert
    SpCas9 R1333A/R1335A
    Use
    Tags
    10X His, TEV protease cleavage site, and maltose-…
    Expression
    Bacterial
    Mutation
    changed arginines 1333 and 1335 to alanine
    Promoter
    Available since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    179528-plasmid-map-sequence-id-351744

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  16. pPLN-hFluc-DM-HA-GFP11-KDEL

    Plasmid
    #177723
    Purpose
    DM Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus
    Depositor
    Erik Böttger
    Article
    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:
    Insert
    hFlucDM
    Use
    Tags
    GFP11, HA, KDEL, and Prolactin Signal Sequence
    Expression
    Bacterial and Mammalian
    Mutation
    R188Q, R261Q (Destabilized Mutant)
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    177723-plasmid-map-sequence-id-352618

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  17. p406 – TDH3-ER-FlucDM-GFP- Ura

    Plasmid
    #177732
    Purpose
    DM Firefly luciferase reporter fused to a KAR2 signal sequence for ER targeting and HDEL for ER retention and fused to eGFP at the C-terminus in yeast plasmid with URA marker
    Depositor
    Erik Böttger
    Article
    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:
    Insert
    FlucDM
    Use
    Tags
    HA, HDEL, KAR2 Signal Sequence, and eGFP
    Expression
    Bacterial and Yeast
    Mutation
    R188Q, R261Q (Destabilized Mutant)
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    177732-plasmid-map-sequence-id-352622

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  21. CMV-10xPP7-Qb model based

    Plasmid
    #158203
    Purpose
    Cassette of 10 different binding sites with high affinity to both PP7 and Qb phage coats protein, as computed by the ML model.
    Depositor
    Roee Amit
    Article
    Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:
    Insert
    10 binding sites with high affinity to PP7 and QB coat proteins based on the machine-learning model
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    158203-plasmid-map-sequence-id-311815

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  31. D24_Ld_171460

    Plasmid
    #135840
    Purpose
    Expression of Leishmania donovani ectodomain in mammalian cells
    Depositor
    Gavin Wright
    Article
    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.
    Insert
    cytochrome b5-like Heme/Steroid binding domain containing protein, putative
    Use
    Tags
    biotinylation sequence and His tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    135840-plasmid-map-sequence-id-274908

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  45. D19_Ld_130940

    Plasmid
    #135835
    Purpose
    Expression of Leishmania donovani ectodomain in mammalian cells
    Depositor
    Gavin Wright
    Article
    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.
    Insert
    subtilisin-like serine peptidase,serine peptidase, clan SB, family S8-like protein
    Use
    Tags
    biotinylation sequence and His tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    135835-plasmid-map-sequence-id-274547

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  46. D9_Ld_190920

    Plasmid
    #135825
    Purpose
    Expression of Leishmania donovani ectodomain in mammalian cells
    Depositor
    Gavin Wright
    Article
    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.
    Insert
    peptidyl-prolyl cis-trans isomerase, macrophage infectivity potentiator precursor, putative
    Use
    Tags
    biotinylation sequence and His tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    135825-plasmid-map-sequence-id-274525

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  48. D4__Ld_290790

    Plasmid
    #135820
    Purpose
    Expression of Leishmania donovani ectodomain in mammalian cells
    Depositor
    Gavin Wright
    Article
    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.
    Insert
    heat shock protein 90, putative,lipophosphoglycan biosynthetic protein, putative,glucose regulated p...
    Use
    Tags
    biotinylation sequence and His tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    135820-plasmid-map-sequence-id-274511

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Showing: 4651 - 4700 of 8813 results