We narrowed to 5,083 results for: mcs

1. EGFP-uTEV1-ACBD5

   Plasmid

   #171008

   Purpose

   HiLITR protease with ACBD5 C-terminal targeting information (peroxisome)

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   EGFP-uTEV1-ACBD5(tmd)
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Promoter

   pTRE-tight (TetOn)

   Available Since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. (517) SB KRAB-SadCas9 U6-gStop

   Plasmid

   #163023

   Purpose

   Sleeping Beauty TET-On expression of CRISPRi with gSTOP gRNA

   Depositor

   Tonia Rex

   Article

   Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

   Insert

   Mammalian codon-optimized SadCas9
   

   Use

   Transposon

   Tags

   KRAB and myc NLS

   Expression

   Mammalian

   Promoter

   Tet-On

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p5A0-T

   Plasmid

   #92016

   Purpose

   Expresses TALE targeted to lac operator without TEV cut sites, anhydrotetracycline inducible TEV protease

   Depositor

   Brian Pfleger

   Article

   Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

   Inserts

   TALE targeting lac operator
   Tobacco Etch Virus Protease
   

   Use

   TALEN

   Tags

   5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

   Mutation

   S219V

   Promoter

   J23102 Promoter (from Anderson promoter library) …

   Available Since

   Jan. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. EGFP-uTEV1-SQStmd

   Plasmid

   #171009

   Purpose

   HiLITR protease with SQS C-terminal targeting information (ER)

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   EGFP-uTEV1-SQS(tmd)
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Promoter

   pTRE-tight (TetOn)

   Available Since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET_RGG-RGG_withTEVsite

   Plasmid

   #124940

   Purpose

   Expression of RGG-RGG

   Depositor

   Matthew Good, Benjamin Schuster, Daniel Hammer

   Article

   Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

   Insert

   RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   with TEV site between RGG domains

   Available Since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCDH-EF1a-tetR-T2A-tSapphire

   Plasmid

   #176312

   Purpose

   construct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome t-Sapphire

   Depositor

   Irmela Jeremias

   Article

   Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

   Inserts

   tetR
   T-sapphire
   

   Use

   Lentiviral

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET_RGG-RFP-RGG

   Plasmid

   #124944

   Purpose

   Exoression of RGG-RFP-RGG

   Depositor

   Matthew Good, Benjamin Schuster, Daniel Hammer

   Article

   Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

   Insert

   RGG-RFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
   

   Tags

   His and mCherry

   Expression

   Bacterial

   Available Since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Pmyo-3::tag:SL1(G20A)

   Plasmid

   #81114

   Purpose

   Expression of exogenous SL1(G20A) in C. elegans muscle

   Depositor

   Xiao Liu

   Article

   Ma et al Nucleic Acids Res. 2016 Aug 23. pii: gkw

   Insert

   sls-1.2 (sls-1.2 Nematode)
   

   Tags

   illumina-adapter

   Expression

   Worm

   Mutation

   G20A

   Promoter

   promoter of myo-3

   Available Since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 360 pCR kdrb

   Plasmid

   #13067

   Depositor

   Nathan Lawson

   Article

   Covassin et al Proc Natl Acad Sci U S A. 2006 Apr 25. 1

   Insert

   kdrb (kdr Zebrafish)
   

   Available Since

   June 18, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pMS1088

    Plasmid

    #80159

    Purpose

    pET-Duet for bacterial expression of Cys-NuG2(4x)-Ald and co-expression of Mycobacterium tuberculosis Formylglycine Generating Enzyme

    Depositor

    Marcelo Sousa

    Article

    Walder et al J Am Chem Soc. 2017 Jul 5. doi: 10.1021/

    Insert

    HisSUMO-Cys-4xNuG2-Ald
    

    Tags

    HisSUMO

    Promoter

    T7

    Available Since

    Aug. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSEM397 - mosTI unc-119 - NNN - operon - gfp - nls - tbb-2

    Plasmid

    #182354

    Purpose

    Nuclear GFP co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::gfp(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Expression

    Worm

    Available Since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSEM399 - mosTI unc-119 - NNN - operon - mScarlet - nls - tbb-2

    Plasmid

    #182356

    Purpose

    Nuclear mScarlet co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::mScarlet(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Expression

    Worm

    Available Since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCI-TPI_WT-xrRNA-4MS2-4H

    Plasmid

    #108370

    Purpose

    Expresses wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag); 4MS2 tethering sites downstream of xrRNA and allows detection via northern blot (4H binding sites)

    Depositor

    Niels Gehring

    Article

    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

    Insert

    Wild type TPI reporter with 4MS2 binding sites downstream of MVE xrRNA and 4H probe binding sites (TPI1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJL1-PorA-4xDQNAT

    Plasmid

    #128392

    Purpose

    In vitro expression of N. meningitidis PorA with C terminal 4xDQNAT glycosylation sites

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    PorA
    

    Tags

    4xDQNAT glycosylation tag and 6xHis tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJL1-TTc-4xDQNAT

    Plasmid

    #128393

    Purpose

    In vitro expression of tetanus toxin fragment C with C terminal 4xDQNAT glycosylation sites

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Tetanus toxin fragment C domain
    

    Tags

    4xDQNAT glycosylation tag and 6xHis tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCI-TPI_WT-4MS2-xrRNA-4H

    Plasmid

    #108371

    Purpose

    Expresses wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag); 4MS2 tethering sites upstream of xrRNA and allows detection via northern blot (4H binding sites)

    Depositor

    Niels Gehring

    Article

    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

    Insert

    Wild type TPI reporter with 4MS2 binding sites upstream of MVE xrRNA and 4H probe binding sites (TPI1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3 PARL-FLAG-CT S70A

    Plasmid

    #13620

    Depositor

    Luca Pellegrini

    Article

    Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

    Insert

    Presenilin-associated rhomboid-like (PARL, PARL Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine 70 to Ala

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

18. p5A7-T

    Plasmid

    #92020

    Purpose

    Expresses TALE targeted to lac operator with one TEV cut site in its N-terminal domain and a second in the C-terminal domain, anhydrotetracycline inducible TEV protease

    Depositor

    Brian Pfleger

    Article

    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

    Inserts

    TALE targeting lac operator with N and C-terminal TEV protease cleavage sites
    Tobacco Etch Virus Protease
    

    Use

    TALEN

    Tags

    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

    Mutation

    S219V

    Available Since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMS1251

    Plasmid

    #80163

    Purpose

    pET-Duet for bacterial expression of Ald-NuG2(4x)-Ald and co-expression of Mycobacterium tuberculosis Formylglycine Generating Enzyme

    Depositor

    Marcelo Sousa

    Article

    Walder et al J Am Chem Soc. 2017 Jul 5. doi: 10.1021/

    Insert

    HisSUMO-Ald-4xNuG2-Ald
    

    Tags

    HisSUMO

    Promoter

    T7

    Available Since

    Aug. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAMBIA1302-Pro35S:Irp9

    Plasmid

    #246319

    Purpose

    Overexpression of a bacterial bifunctional salicylate synthase targeted to the cytosol

    Depositor

    Chung-Jui Tsai

    Article

    Xue et al Plant Cell. 2013 Jul;25(7):2714-30. doi:

    Insert

    Irp9
    

    Expression

    Plant

    Promoter

    cauliflower mosaic virus 35S

    Available Since

    Oct. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge