Addgene

We narrowed to 6,477 results for: sas

Showing: 1861 - 1880 of 6477 results

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pNUT N6His Y95F/Y188F hTFNG

Plasmid

#69601

Purpose

Expresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in the N-lobe

Depositor

Anne Mason, Ross MacGillivray

Article

Mason et al Protein Expr Purif. 2004 Aug;36(2):318-2

Insert

mutated human serum transferrin (TF Human)

Use

Tags

Hexa His tag and N-terminal signal peptide, 4 aa …

Expression

Mammalian

Mutation

Asn413 Asp, Asn611Asp, Tyr95Phe, Tyr188Phe

Promoter

SV40

Available since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ADAM7 (H243Y)

Plasmid

#31138

Depositor

Yardena Samuels

Article

Wei et al Hum Mutat. 2011 Jun;32(6):E2148-75. doi:

Insert

ADAM7 (ADAM7 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Histidine 243 changed to Tyrosine

Promoter

Available since

Sept. 1, 2011

Availability

Academic Institutions and Nonprofits only
pDONR221-hSOX10-C373T

Plasmid

#24734

Depositor

William Pavan

Article

Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

Insert

(sex determining region Y)-box 10 (SOX10 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

C replaced by T at position 373 resulting in Q125…

Promoter

Available since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
pDONR221-hSOX10-C1271T

Plasmid

#24736

Depositor

William Pavan

Article

Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

Insert

(sex determining region Y)-box 10 (SOX10 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

C replaced with T at position 1271 resulting in A…

Promoter

Available since

May 18, 2010

Availability

Academic Institutions and Nonprofits only
pLV.tetO.Nurr1

Plasmid

#234848

Purpose

3rd generation lentiviral vector, expresses Nurr1 under control of TetON promoter

Depositor

Malin Parmar

Article

Nolbrant et al Stem Cell Reports. 2020 Oct 13;15(4):869

Insert

Nurr1 (Nr4a2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLV.tetO.lmx1a

Plasmid

#234847

Purpose

3rd generation lentiviral vector, expresses Lmx1a under control of TetON promoter

Depositor

Malin Parmar

Article

Nolbrant et al Stem Cell Reports. 2020 Oct 13;15(4):869

Insert

Lmx1a (Lmx1a Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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BtARR1 Cys-less

Plasmid

#236274

Purpose

Bacterial expression of bovine arrestin-1 C63A, C128S, C143A, inserted GGT codon after starting ATG

Depositor

Vsevolod Gurevich

Article

Hanson et al Proc Natl Acad Sci U S A. 2006 Mar 28;10

Insert

arrestin-1 (SAG Bovine)

Use

Tags

Expression

Bacterial

Mutation

C63A, C128S, C143A, inserted GGT codon after star…

Promoter

lac

Available since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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p3HAT1Luc1

Plasmid

#236275

Purpose

Mammalian expression of human angiotensin II type 1 receptor; N-terminal HA tag, C-terminal Rluc8

Depositor

Vsevolod Gurevich

Article

Gimenez et al J Biol Chem. 2012 Aug 24;287(35):29495-5

Insert

angiotensin II type 1 receptor (AGTR1 Human)

Use

Tags

3xHA and Rluc8

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pJET-CMV-GFP-P2A-K20-P2A-mCherry

Plasmid

#185618

Purpose

Ribosomal stalling reporter: positive control

Depositor

Sabine Fuchs

Article

Kok et al Nucleic Acids Res. 2025 Jan 7;53(1):gkae

Insert

GFP-K20(AAA)-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pJET-CMV-GFP-P2A-K0-P2A-mCherry

Plasmid

#185619

Purpose

Ribosomal stalling reporter: empty

Depositor

Sabine Fuchs

Article

Kok et al Nucleic Acids Res. 2025 Jan 7;53(1):gkae

Insert

GFP-empty-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pJET-CMV-GFP-P2A-I15-P2A-mCherry

Plasmid

#185620

Purpose

Ribosomal stalling reporter: 15x isoleucine

Depositor

Sabine Fuchs

Article

Kok et al Nucleic Acids Res. 2025 Jan 7;53(1):gkae

Insert

GFP-I15-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNAintron-CHIKV-Structural polyprotein with Nluc tagged E2

Plasmid

#215699

Purpose

Produces the Chikungunya virus structural polyprotein Capsid-E3-NLucE2-6K-E1

Depositor

Melinda Brindley

Article

Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

Insert

CHIKV structural polyprotein Capsid-E3-NLucE2-6K-E1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-ArgiNLS-EGFP

Plasmid

#220594

Purpose

Cre-dependent expression of a single-cell discriminating version of EGFP fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

EGFP

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-ArgiNLS-miRFP670

Plasmid

#220600

Purpose

Constitutive expression of a single-cell discriminating version of miRFP670 fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

miRFP670

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-ArgiNLS-mKate2

Plasmid

#220599

Purpose

Constitutive expression of a single-cell discriminating version of mKate2 fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

mKate2

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-ArgiNLS-AausFP1

Plasmid

#220595

Purpose

Constitutive expression of a single-cell discriminating version of AausFP1 fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

AausFP1

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLEX-ArgiNLS-EGFP

Plasmid

#220601

Purpose

Cre-dependent expression of a single-cell discriminating version of EGFP fluorescent protein

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

EGFP

Use

AAV and Cre/Lox

Tags

ArgiNLS

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pLC045 - pL0_CrGLK (CDS1ns)

Plasmid

#203904

Purpose

MoClo compatible L0 plasmid containing the CrGLK coding sequence, lacking a stop codon to allow for C-terminal fusions.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

CrGLK (without a stop codon)

Use

Tags

Expression

Plant

Mutation

Silent mutation of BpiI or BsaI cut site for MoCl…

Promoter

Available since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLC047 - pL2_p2x35s-CrGLK-tMAS

Plasmid

#203906

Purpose

Overexpression of the CrGLK coding sequence from C. roseus in a plant binary vector for Agrobacterium-mediated transformation.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

CrGLK

Use

Tags

Expression

Plant

Mutation

Silent mutation of BpiI or BsaI cut site for MoCl…

Promoter

CaMV 2x35S

Available since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSB278 - pL0_pT16H2 (pro + 5U)

Plasmid

#203889

Purpose

T16H2 promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

T16H2 promoter and 5'UTR

Use

Tags

Expression

Plant

Mutation

Mutation of BpiI or BsaI cut site for MoClo domes…

Promoter

Available since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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