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pET28-sfGFP[D134TAG+N150TAA]

Plasmid

#164582

Purpose

superfolder GFP with D134TAG and N150TAA mutations

Depositor

Ryan Mehl

Article

Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

Insert

sfGFP-134TAG+150TAA

Tags

6xHis

Expression

Bacterial

Mutation

D134TAG + N150TAA Mutations

Promoter

T7

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-EF1A-DIO-PSD95.FingR-eGFP-CCR5TC

Plasmid

#126216

Purpose

Labeling of excitatory synapses (green fluorescence) in cre-expressing neurons

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

PSD95.FingR-eGFP-CCR5TC

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1A

Available Since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pFastBac-Dual_FluA/H7N9

Plasmid

#229860

Purpose

pFastBac Dual vector for co-expression of FluPolA/H7N9 polymerase heterotrimer (PA/PB1/PB2)

Depositor

Stephen Cusack

Article

Kouba et al Cell Rep. 2023 Jan 31;42(1):111901. doi:

Inserts

PA_H7N9-FluA
PB1_H7N9-FluA
PB2_H7N9-FluA

Tags

2x-Streptag and 8xHis-tag

Expression

Insect

Available Since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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4XCLEAR-luciferase reporter

Plasmid

#66800

Purpose

Reporter for activity of the CLEAR network

Depositor

Albert La Spada

Article

Cortes et al Nat Neurosci. 2014 Sep;17(9):1180-9. doi

Insert

4XCLEAR

Use

Luciferase

Promoter

Four CLEAR elements (GTCACGTGAC) in tandem derive…

Available Since

Nov. 5, 2015

Availability

Academic Institutions and Nonprofits only

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CMV_3xFLAG_NLS_ISCro4_NLS_T2A_puroR_SV40_polyA

Plasmid

#251494

Purpose

Expression of a human codon optimised ISCro4 bridge recombinase

Depositor

Martin Jinek

Article

Pelea et al Science. 2026 Feb 5:eadz1884. doi: 10.11

Insert

ISCro4 bridge recombinase

Expression

Mammalian

Mutation

NA

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pTRIPZ shNS

Plasmid

#127696

Purpose

Doxycyclin inducible pTRIPZ lentivirus vector containing a non-silencing sequence for both human and mouse

Depositor

Sandra Demaria

Article

Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

Insert

shNS

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAnc80L65AAP

Plasmid

#92307

Purpose

Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct with endogenous AAP

Depositor

Luk Vandenberghe

Article

Landegger et al Nat Biotechnol. 2017 Mar;35(3):280-284.

Insert

Ancestral AAV Capsid Anc80L65AAP

Use

AAV and Synthetic Biology

Expression

Mammalian

Promoter

Rep2

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pC1-HyPerFLEX-ERlum

Plasmid

#238941

Purpose

Mammalian expression of HyPerFLEX targeted to the luminal side of the endoplasmic reticulum membranes

Depositor

Vsevolod Belousov

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX-ERlum (Calreticulin signal sequence-HyPerFLEX-KDEL)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-EF1A-DIO-Gephyrin.FingR-eGFP-CCR5TC

Plasmid

#126217

Purpose

Labeling of inhibitory synapses (green fluorescence) in cre-expressing neurons

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

Gephyrin.FingR-eGFP-CCR5TC

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1A

Available Since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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T7_MBP_TEV_ISCro4_6xHis_T7_term

Plasmid

#251502

Purpose

Bacterial expression vector for producing recombinant ISCro4 (E. coli codon optimised)

Depositor

Martin Jinek

Article

Pelea et al Science. 2026 Feb 5:eadz1884. doi: 10.11

Insert

ISCro4 bridge recombinase

Expression

Bacterial

Mutation

NA

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pQTEV-PSMD10

Plasmid

#31332

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

PSMD10, proteasome (prosome, macropain) 26S subunit, non-ATPase, 10 (gankyrin) (PSMD10 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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Z-lock cofilin

Plasmid

#141137

Purpose

controlling the actin-severing ability of cofilin with light

Depositor

Klaus Hahn

Article

Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

Insert

Zdk2, Cofilin, LOV2

Expression

Mammalian

Mutation

mCherry, Zdk2(132F), Cofilin S3A, LOV WT

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-cFos-tTA-pA

Plasmid

#66794

Purpose

Expresses TetActivator protein under the control of the c-fos promoter for use in TetTaging

Depositor

William Wisden

Article

Zhang et al Nat Neurosci. 2015 Apr;18(4):553-61. doi

Insert

cfos promoter linked to the tet activator gene

Use

AAV

Tags

no

Expression

Mammalian

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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FLAG NC

Plasmid

#214675

Purpose

Negative control plasmid for splicing reporters consisting of MCP fused FLAG epitope tags

Depositor

Eugene Yeo

Article

Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

FLAG

Tags

MCP and V5

Expression

Mammalian

Promoter

EF-1a

Available Since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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pDA077

Plasmid

#131388

Purpose

L1RP expression

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

L1RP

Expression

Mammalian

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-NMNAT2

Plasmid

#133260

Purpose

Express FLAG-tagged NMNAT2 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Insert

NMNAT2 (NMNAT2 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pPD0029_pIVTRup(BsmBI)_SpCas9

Plasmid

#242535

Purpose

IVT

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

SpCas9

Use

CRISPR

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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TagRFP-T_A1aY1

Plasmid

#158751

Purpose

Lentiviral vector for expression of TagRFP-T_A1aY1 in mammalian cells for detection of tyrosinated microtubules.

Depositor

Minhajuddin Sirajuddin

Article

Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

Insert

TagRFP-T_A1aY1

Use

Lentiviral

Tags

TagRFP-T

Expression

Mammalian

Promoter

CAG (CMV enhancer and chicken beta actin promoter)

Available Since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pSG019

Plasmid

#126502

Purpose

Pseudomonas aeruginosa disaggregase ClpG cloned as ClpG-6xHis

Depositor

Ute Romling

Article

Lee et al Proc Natl Acad Sci U S A. 2018 Jan 9;115

Insert

disaggregase ClpG with 6xHis-tag

Tags

C-terminal 6xHis

Expression

Bacterial

Promoter

PBAD

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pNluc-hbb

Plasmid

#239753

Purpose

Template for in vitro transcription of nanoluciferase flanked by the human haemoglobin beta 5' and 3' UTRs.

Depositor

Catherine Jopling

Article

Smart et al Mol Ther Nucleic Acids. 2025 Jun 24;36(3

Insert

Nanoluciferase flanked by human haemoglobin beta 5' and 3' UTRs

Use

Luciferase

Promoter

T7 (included as part of insert)

Available Since

July 7, 2025

Availability

Academic Institutions and Nonprofits only

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