We narrowed to 4,135 results for: p2a

1. pPL6873_2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS

   Plasmid

   #217970

   Purpose

   Provides expression from a CMV promoter surrounded by insulators. Integrates at FRT site. Confers puromycin resistance. Expresses nuclear localized BFP.

   Depositor

   Robert Piper

   Insert

   2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-CMV-Nier1s-3NLS-P2A-EGFP-3NLS

   Plasmid

   #217478

   Purpose

   Mammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1s

   Depositor

   Peng Zou

   Article

   Lin et al Cell Rep Methods. 2023 Jul 27;3(8):10054

   Insert

   Nier1s
   

   Use

   Tags

   3xNLS, P2A-EGFP-3xNLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-CMV-Nier1b-3NLS-P2A-EGFP-3NLS

   Plasmid

   #217479

   Purpose

   Mammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1b

   Depositor

   Peng Zou

   Article

   Lin et al Cell Rep Methods. 2023 Jul 27;3(8):10054

   Insert

   Nier1b
   

   Use

   Tags

   3xNLS, P2A-EGFP-3xNLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCF827-recipient_U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

   Plasmid

   #211692

   Purpose

   U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

   Depositor

   Christof Fellmann

   Article

   Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

   Insert

   SpyCas9 and guide RNA recipient vector
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCF826-recipient_U6-sgRNA-EFS-Cas9-P2A-mCherry2

   Plasmid

   #211688

   Purpose

   U6-sgRNA-EFS-Cas9-P2A-mCherry2

   Depositor

   Christof Fellmann

   Article

   Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

   Insert

   SpyCas9 and guide RNA recipient vector
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-h.Rhno1 T52A (B)-C-Myc-DDK-P2A-Puro

   Plasmid

   #211621

   Purpose

   Rhno1 T52A

   Depositor

   Agnel Sfeir

   Article

   Brambati et al Science. 2023 Aug 11;381(6658):653-660.

   Insert

   hRHNO1 (RHNO1 Human)
   

   Use

   Tags

   myc-flag

   Expression

   Mammalian

   Mutation

   T52A

   Promoter

   CMV

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-h.Rhno1 S51A (A)-C-Myc-DDK-P2A-Puro

   Plasmid

   #211620

   Purpose

   Rhno1 S51A

   Depositor

   Agnel Sfeir

   Article

   Brambati et al Science. 2023 Aug 11;381(6658):653-660.

   Insert

   hRHNO1 (RHNO1 Human)
   

   Use

   Tags

   myc-flag

   Expression

   Mammalian

   Mutation

   S51A

   Promoter

   CMV

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-h.Rhno1 S163A (E)-C-Myc-DDK-P2A-Puro

   Plasmid

   #211624

   Purpose

   Rhno1 S163A

   Depositor

   Agnel Sfeir

   Article

   Brambati et al Science. 2023 Aug 11;381(6658):653-660.

   Insert

   hRHNO1 (RHNO1 Human)
   

   Use

   Tags

   myc-flag

   Expression

   Mammalian

   Mutation

   S163A

   Promoter

   CMV

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-h.Rhno1 S162A (D)-C-Myc-DDK-P2A-Puro

   Plasmid

   #211623

   Purpose

   Rhno1 S162A

   Depositor

   Agnel Sfeir

   Article

   Brambati et al Science. 2023 Aug 11;381(6658):653-660.

   Insert

   hRHNO1 (RHNO1 Human)
   

   Use

   Tags

   myc-flag

   Expression

   Mammalian

   Mutation

   S162A

   Promoter

   CMV

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-h.Rhno1 S164A (F)-C-Myc-DDK-P2A-Puro

    Plasmid

    #211625

    Purpose

    Rhno1 S164A

    Depositor

    Agnel Sfeir

    Article

    Brambati et al Science. 2023 Aug 11;381(6658):653-660.

    Insert

    hRHNO1 (RHNO1 Human)
    

    Use

    Tags

    myc-flag

    Expression

    Mammalian

    Mutation

    S164A

    Promoter

    CMV

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-h.Rhno1 S178A (G)-C-Myc-DDK-P2A-Puro

    Plasmid

    #211626

    Purpose

    Rhno1 S178A

    Depositor

    Agnel Sfeir

    Article

    Brambati et al Science. 2023 Aug 11;381(6658):653-660.

    Insert

    hRHNO1 (RHNO1 Human)
    

    Use

    Tags

    myc-flag

    Expression

    Mammalian

    Mutation

    S178A

    Promoter

    CMV

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5-FRT-TO-NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7

    Plasmid

    #212805

    Purpose

    Constitutive or doxycycline-inducible expression of NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7 in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV/CBA::EGFP-P2A-tC3/54aa-FLAG

    Plasmid

    #129479

    Purpose

    AAV2 transfer plasmid for truncated C3 (54 aa) tagged with FLAG with EGFP under control of the CBA promoter

    Depositor

    Robert Gross

    Insert

    truncated (AA148-201) exoenzyme C3 from C. botulinum, P2A, EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBA

    Available since

    Feb. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. AttB_ACE2[R.alcyone+ferrum]-2A-TMPRSS2_IRES-iRFP670-P2A-PuroR

    Plasmid

    #190079

    Purpose

    express chimeric Rhinolophus alcyone ACE2 and human TMPRSS2 with selectable markers iRFP670 and puromycin resistance gene

    Depositor

    Kenneth Matreyek

    Article

    Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

    Insert

    ACE2 (TMPRSS2 Rhinolophus alcyone and Rhinolophus ferrumequinum, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    none

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-TagBFP-P2A-Pre-SPHERE-Rncp-iGluSnFR1

    Plasmid

    #187093

    Purpose

    Glutamate sensor for localization to membrane contatct

    Depositor

    Kotaro Oka

    Article

    Shindo et al bioRxiv 2022

    Insert

    Pre-SPHERE-Rncp-iGluSnFR1
    

    Use

    Tags

    TagBFP-P2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-iRFP-P2A-Post-SPHERE-Rncp-iGluSnFR1

    Plasmid

    #187094

    Purpose

    Glutamate sensor for localization to membrane contatct

    Depositor

    Kotaro Oka

    Article

    Shindo et al bioRxiv 2022

    Insert

    Post-SPHERE-Rncp-iGluSnFR1
    

    Use

    Tags

    iRFP670-P2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-mCherry-P2A-Pre-SPHERE-sfmTurquoise2-iGluSnFR

    Plasmid

    #187095

    Purpose

    Glutamate sensor for localization to membrane contatct

    Depositor

    Kotaro Oka

    Article

    Shindo et al bioRxiv 2022

    Insert

    Pre-SPHERE-sfmTurquoise2-iGluSnFR
    

    Use

    Tags

    mCherry-P2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVH270-Tier1-OTtgO2-PCMVmin2-SEAP-p2A-iRFP670

    Plasmid

    #169592

    Purpose

    Tier-1 vector encoding PTtgO2-driven SEAP-p2A-iRFP670 expression (OTtgO2-PCMVmin-2-SEAP-p2A-iRFP670-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    phloretin-controlled SEAP and iRFP expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TtgO2-PCMVmin-2

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pVH269-Tier1-OVanO2-PCMVmin2-SEAP-p2A-iRFP670

    Plasmid

    #169667

    Purpose

    Tier-1 vector encoding PVanO2-driven SEAP-p2A-iRFP670 expression (OVanO2-PCMVmin-2-SEAP-p2A-iRFP670-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    vanillic acid-controlled SEAP and iRFP expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    VanO2-PCMVmin

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. SPELL-pBabe-Vav2(DPZ) (Component 2-T2AP2A-Component 1)

    Plasmid

    #170488

    Purpose

    SPELL Vav2(DH-PH-ZnF). Two components cloned into the same plasmid with T2A/P2A sequence. Component 2-T2A-P2A-Component 1

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Nat Commun. 2018 Oct 2;9(1):4042. doi: 1

    Insert

    FRB, Vav2(C-lobe), iFKBP, Vav2(N-lobe), mCherry
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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