We narrowed to 2,482 results for: PRK

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pLpA-DN.JNK1

Plasmid

#51942

Purpose

Adenoviral vector for dominant negative JNK1 expression

Depositor

Jeffery Molkentin

Article

Liang et al EMBO J. 2003 Oct 1;22(19):5079-89.

Insert

DN JNK1 (Mapk8 Mouse)

Use

Adenoviral

Tags

FLAG

Mutation

dominant negative: the dual phosphorylation moti…

Promoter

CMV

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only
HA-MAP1LC3A

Plasmid

#137756

Purpose

Expression of LC3A

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

MAP1LC3A (MAP1LC3A Human)

Tags

HA and myc

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLL3.7m-psMEK1

Plasmid

#89361

Purpose

A lenti-viral vector expressing photoswitchable MEK1 in mammalian cells

Depositor

Michael Lin

Article

Zhou et al Science. 2017 Feb 24;355(6327):836-842.

Insert

psMEK1 (MAP2K1 Human, Synthetic)

Use

Lentiviral

Tags

Human influenza hemagglutinin (HA) tag and pdDron…

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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myc-Atg14

Plasmid

#86747

Purpose

N-terminal myc-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

Insert

human Atg14 (ATG14 Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pFBD-Akt13C(S473D)

Plasmid

#86577

Purpose

Expresses cleavable human full-length Akt1(S473D) phosphomimetic

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Tags

10xHis

Expression

Insect

Mutation

3C cleavage site inserted at pos. 130; S473D phos…

Available Since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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pLEX-Frb:Akt2-IRES-Hygromycin

Plasmid

#120713

Purpose

Expresses Frb:Akt2 fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

AKT2 (AKT2 Human)

Use

Lentiviral

Tags

FRB(T2098L)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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904 pLNCX HA Akt1 K179M

Plasmid

#9007

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

Akt1 K179M (AKT1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

K179M. Kinase dead.

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only
MBP-MEK1

Plasmid

#68300

Purpose

N-terminal MBP tagged human MEK1 gene for bacterial expression

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

MBP-MEK1 (MAP2K1 Human, Synthetic)

Tags

His and MBP

Expression

Bacterial

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-CIB1-Myc

Plasmid

#32505

Depositor

John Groffen, Nora Heisterkamp

Article

Haataja et al J Biol Chem. 2002 Mar 8;277(10):8321-8.

Insert

CIB1 (CIB1 Human)

Tags

Myc

Expression

Mammalian

Available Since

Jan. 26, 2012

Availability

Academic Institutions and Nonprofits only
pDEST47-SARA

Plasmid

#67473

Purpose

Mammalian expression of hSara with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag p38 beta2 (agf)

Plasmid

#20356

Depositor

Roger Davis

Article

Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

Insert

pDCNA3 Flag p38 beta(agf) (MAPK11 Human)

Tags

Flag

Expression

Mammalian

Mutation

Replace Thr 182 and Tyr 184 with Ala and Phe resp…

Available Since

May 11, 2009

Availability

Academic Institutions and Nonprofits only
pLpA-DN.JNK2

Plasmid

#51943

Purpose

Adenoviral vector for dominant negative JNK2 expression

Depositor

Jeffery Molkentin

Article

Liang et al EMBO J. 2003 Oct 1;22(19):5079-89.

Insert

DN JNK2 (Mapk9 Mouse)

Use

Adenoviral

Tags

HA

Mutation

dominant negative: the dual phosphorylation moti…

Promoter

CMV

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only
PH Akt-Venus

Plasmid

#85223

Purpose

Venus tagged PH domain of Akt for use as a PIP3 sensor

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

Insert

PH AKT(1-148) (AKT1 Human)

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-Akt1-aa1-149

Plasmid

#73410

Purpose

Expresses HA tagged aa1-149 of AKT1

Depositor

Jie Chen

Article

Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

Insert

AKT1 (AKT1 Human)

Tags

HA tag

Expression

Mammalian

Mutation

Expresses aa 1-149 only of AKT1

Promoter

CMV

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
1071 pBabe puroL myr Akt T308A S473A

Plasmid

#9014

Depositor

William Sellers

Article

DiRenzo et al Cancer Res. 2002 Jan 1. 62(1):89-98.

Insert

Akt1 T308A S473A (AKT1 Human)

Use

Retroviral

Tags

HA and Myr

Expression

Mammalian

Mutation

T308A S473A

Available Since

Nov. 3, 2005

Availability

Academic Institutions and Nonprofits only
myc-KIF5A

Plasmid

#127616

Purpose

N-terminal myc-tagged mouse KIF5A

Depositor

Josef Kittler

Article

Twelvetrees et al Neuron. 2010 Jan 14;65(1):53-65. doi: 10

Insert

KIF5A (Kif5a Mouse)

Tags

myc

Expression

Mammalian

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-Akt1-aa120-433

Plasmid

#73411

Purpose

Expresses HA tagged aa120-433 AKT1

Depositor

Jie Chen

Article

Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

Insert

AKT1 (AKT1 Human)

Tags

HA tag

Expression

Mammalian

Mutation

Expresses aa 120-433 of AKT1

Promoter

CMV

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pFL-SV40-p38 β/MAPK11 5'-UTR

Plasmid

#115357

Purpose

firefly luciferase (Fluc) reporter containing the 5’-UTR of p38 β/MAPK11 mRNA

Depositor

Ming-Chih Lai

Article

Ku et al Mol Cell Biol. 2018 Dec 11;39(1). pii: M

Insert

p38 β/MAPK11 5'-UTR (MAPK11 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AktAR

Plasmid

#61624

Purpose

FRET-based biosensor for monitoring Akt activity

Depositor

Jin Zhang

Article

Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

Insert

AktAR (AKT1 Synthetic, Human, Budding Yeast)

Tags

Cerulean and cpVenus[E172]

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST47-ARL4C-GFP

Plasmid

#67402

Purpose

Mammalian expression of hArl4c with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL7 (ARL4C Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only