We narrowed to 8,785 results for: sgrna

1. pDonor_hU6

   Plasmid

   #69312

   Purpose

   sgRNA scaffold and human U6 promoter

   Depositor

   Andrea Ventura

   Article

   Vidigal et al Nat Commun. 2015 Aug 17;6:8083. doi: 10.

   Insert

   hU6 promoter and sgRNA scaffold flanked by BbsI sites
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   human U6 promoter

   Available Since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQdCas12a.luxR(mut)-sggfp(C)

   Plasmid

   #236043

   Purpose

   The plasmid pQdCas12a.luxR(mut)-sggfp(C) expresses the dCas12a endonuclease and the sgRNA (design C) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.

   Depositor

   Radhakrishnan Mahadevan

   Insert

   quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (C) of gfp gene
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   quorum sensing promoter

   Available Since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTK473

   Plasmid

   #114715

   Purpose

   sgRNA for LGN's N-terminal locus

   Depositor

   Tomomi Kiyomitsu

   Article

   Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

   Insert

   LGN sgRNA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pV1090

   Plasmid

   #111427

   Purpose

   gRNA entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at RPS1 (Part of Duet system)

   Depositor

   Gerald Fink

   Article

   Vyas et al Sci Adv. 2015;1(3):e1500248.

   Insert

   sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMLS134

   Plasmid

   #73714

   Purpose

   SapTrap destination vector for building sgRNA expression vectors.

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   pU6::sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   SapI insertion site

   Promoter

   C. elegans U6 snRNA pol III promoter

   Available Since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJW1259

   Plasmid

   #61251

   Purpose

   Cas9 plasmid with sgRNA deleted; for use in C. elegans

   Depositor

   Jordan Ward

   Article

   Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

   Insert

   Deletion
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Deleted sgRNA(F+E) sequence from pJW1219

   Promoter

   Available Since

   Dec. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEX-A-U6-TelgRNA_PuroR

   Plasmid

   #84782

   Purpose

   Expresses telomere-specific sgRNA.

   Depositor

   Heinrich Leonhardt

   Article

   Schmidtmann et al Nucleus. 2016 Sep 27:0.

   Insert

   telomere-specific sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMLS256

   Plasmid

   #73715

   Purpose

   SapTrap destination vector for building combined sgRNA expression + repair template vectors

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   pU6::sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   SapI insertion site

   Promoter

   C. elegans U6 snRNA pol III promoter

   Available Since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. ttHsCas12a

   Plasmid

   #182385

   Purpose

   Gives high editing efficiency in barley.

   Depositor

   Wendy Harwood

   Article

   Lawrenson et al bioRxiv 2022.04.28.489853

   Insert

   ttHsCas12a
   

   Use

   CRISPR; Coding sequence only

   Tags

   Expression

   Mutation

   D156R

   Promoter

   None

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTK372

    Plasmid

    #114687

    Purpose

    sgRNA targeting NuMA's C-terminal locus

    Depositor

    Tomomi Kiyomitsu

    Article

    Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

    Insert

    NuMA sgRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJMP2849

    Plasmid

    #160677

    Purpose

    Mobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAs

    Depositor

    Jason Peters

    Article

    Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJ2048.2

    Plasmid

    #140687

    Purpose

    3-color stripe (different sgRNAs)

    Depositor

    Yolanda Schaerli

    Article

    Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

    Insert

    3-color stripe (different sgRNAs)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWT055g

    Plasmid

    #96864

    Purpose

    sgRNA-HEK3

    Depositor

    David Liu

    Article

    Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

    Insert

    sgRNA-HEK3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJMP2782

    Plasmid

    #160668

    Purpose

    Mobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAs

    Depositor

    Jason Peters

    Article

    Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMZ284

    Plasmid

    #52225

    Purpose

    Expression of sgRNA precursor targeted against ade6+

    Depositor

    Mikel Zaratiegui

    Article

    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

    Insert

    rrk1-sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    rrk1

    Available Since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pWT055h

    Plasmid

    #96867

    Purpose

    sgRNA-HEK4

    Depositor

    David Liu

    Article

    Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

    Insert

    sgRNA-HEK4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pWT055e

    Plasmid

    #96866

    Purpose

    sgRNA-EMX1

    Depositor

    David Liu

    Article

    Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

    Insert

    sgRNA-EMX1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pWT055f

    Plasmid

    #96865

    Purpose

    sgRNA-FANCF

    Depositor

    David Liu

    Article

    Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

    Insert

    sgRNA-FANCF
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pWT062d

    Plasmid

    #107911

    Purpose

    W2m.2-4 plasmid. Contains H1-TetR-sgRNA B and is ppart of the CAMERA2m system for cellular recording in mammalian cells

    Depositor

    David Liu

    Article

    Tang et al Science. 2018 Feb 15. pii: science.aap89

    Insert

    H1-TetR-sgRNA B
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pWT062e

    Plasmid

    #107910

    Purpose

    W2m.2-3 plasmid. Contains U6-LacI-sgRNA A and is ppart of the CAMERA2m system for cellular recording in mammalian cells

    Depositor

    David Liu

    Article

    Tang et al Science. 2018 Feb 15. pii: science.aap89

    Insert

    U6-LacI-sgRNA A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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