We narrowed to 7,308 results for: RAP

1. pET11a-Z-NGFP

   Plasmid

   #52734

   Purpose

   IPTG-inducible expression of Z-NGFP positive control for in vivo split GFP assembly assay

   Depositor

   Lynne Regan

   Article

   Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

   Insert

   NGFP+Z-fusion
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTcI42-Wasabi

   Plasmid

   #86118

   Purpose

   mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI42

   Depositor

   Mikhail Shapiro

   Article

   Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

   Inserts

   TcI42
   mWasabi
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A67T, K6N, S33T, Y61H, L119P, F122C

   Promoter

   pR/pL and pR/pL, LacI

   Available since

   Feb. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. Ii-Str_SBP-EGFP-GPI

   Plasmid

   #65296

   Purpose

   synchronize trafficking of EGFP-GPI from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   GPI anchor
   

   Use

   Tags

   EGFP and IL-2 Signal Sequence and Streptavidin Bi…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMP71-CD40L

   Plasmid

   #174595

   Purpose

   Stably expresses the extracellular and transmembrane domain of murine CD40L

   Depositor

   Stanley Riddell

   Article

   Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

   Insert

   CD40L
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Flag pLJM1 Rheb1

   Plasmid

   #19312

   Depositor

   David Sabatini

   Article

   Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

   Insert

   RHEB Ras homolog enriched in brain (RHEB Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

6. TOPO GFP

   Plasmid

   #68488

   Purpose

   TOPO construct expressing GFP for generation of GMAP-compatible gene

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   Green Fluorescent Protein
   

   Use

   Gmap

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Plac

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRK5 HA-RagC(S75N)

   Plasmid

   #99719

   Purpose

   Mammalian expression of HA-RagC(S75N)

   Depositor

   Kuang Shen, David Sabatini

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Insert

   RagC (RRAGC )
   

   Use

   Tags

   HA

   Expression

   Mutation

   S75N

   Promoter

   CMV

   Available since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTriEx-NLyn-Venus-Zdk1

   Plasmid

   #81042

   Purpose

   mammalian expression of plasma-membrane anchored Zdk1

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Insert

   Zdk1
   

   Use

   Tags

   6x His tag, FLAG, NLyn, and mVenus

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUREF-EX

   Plasmid

   #199331

   Purpose

   A pEF-BOS-EX-derived expression vector that contains an SV40 promoter-driven puromycin resistance gene

   Depositor

   Rikiro Fukunaga

   Article

   Fujii et al Genes Cells. 2023 Mar 2. doi: 10.1111/gt

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pME-BrainbowTEC

    Plasmid

    #82405

    Purpose

    Gateway-compatible Brainbow-1.0 with fluorophores tdTomato-myc/EGFP/E2Crimson-HA

    Depositor

    Judith Eisen

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Inserts

    tdTomatoMYC fusion
    EGFP
    E2CrimsonHA
    

    Use

    Tags

    HA and MYC

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pH6HTC_PTBP1-Halo

    Plasmid

    #175302

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    PTBP1 (PTBP1 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p3xFLAG-CMV10-hTti1

    Plasmid

    #30213

    Depositor

    Noboru Mizushima

    Article

    Kaizuka et al J Biol Chem. 2010 Jun 25. 285(26):20109-

    Insert

    Tel2 interacting protein 1 (TTI1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pLKO.1 RagD shRNA #1

    Plasmid

    #30321

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    RagD shRNA (RRAGD Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 2, 2011

    Availability

    Academic Institutions and Nonprofits only

14. p458 VQR

    Plasmid

    #101727

    Purpose

    Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VQR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    VQR

    Promoter

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDM1081

    Plasmid

    #108981

    Purpose

    Knockout cassette Hygromycin

    Depositor

    Rob Kay

    Article

    Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

    Insert

    Knockout cassette Hygromycin
    

    Use

    Cre/Lox; Dictyostelium discoideum knockout vector

    Tags

    Expression

    Mutation

    Promoter

    coaA

    Available since

    July 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMP71-IFN-B

    Plasmid

    #174597

    Purpose

    Expresses secreted murine interferon beta

    Depositor

    Stanley Riddell

    Article

    Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

    Insert

    secreted IFN-beta
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTcI-Wasabi

    Plasmid

    #86101

    Purpose

    mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TcI
    mWasabi
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A67T

    Promoter

    pR/pL and pR/pL, LacI

    Available since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDEST-CMV-N-Tandem-mCherry-EGFP

    Plasmid

    #123216

    Purpose

    N-terminal Tandem mCherry EGFP tag. Gateway destination vector for mammalian expression.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MTK2_005

    Plasmid

    #123700

    Purpose

    Encodes pCAG as a Type 2 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    pCAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pME-GFP-P2A

    Plasmid

    #80807

    Purpose

    GFP without stop codon followed by porcine teschovirus-1 2A (P2A) "self-cleaving" peptide

    Depositor

    Philip Washbourne

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    GFP-P2A
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge