Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 9,218 results for: Set

Clear keywords
Showing: 1941 - 1960 of 9218 results

  1. Ai62(TITL-tdT) Flp-in replacement vector

    Plasmid
    #61576
    Purpose
    Recombinase-mediated cassette exchange in ES cells to insert a Cre & tTA-dependent tdTomato expression cassette into a docking site within the mouse TIGRE genomic locus
    Depositor
    Hongkui Zeng
    Article
    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10
    Insert
    chromatin insulator flanked TRE-LSL-tdTomato
    Use
    Recombinase-mediated cassette exchange using flp …
    Available Since
    March 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    61576-plasmid-map-sequence-id-209910

    Enlarge

  3. pINSPECT_SigP:NLuc

    Plasmid
    #197263
    Purpose
    Cloning Backbone for INSPECT expression. Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via Esp3I.
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676
    Type
    Empty backbone
    Use
    CRISPR, Luciferase, Synthetic Biology, and Unspec…
    Expression
    Mammalian
    Available Since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    197263-plasmid-map-sequence-id-389724

    Enlarge

  4. pINSPECT_5'-xrRNA_NLuc_3'-HCV-UTR

    Plasmid
    #197265
    Purpose
    Cloning Backbone for enhanced INSPECT expression (5' xrRNA + 3' HCV-UTR). Encodes for intronic IRES-driven NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via BsaI.
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676
    Type
    Empty backbone
    Use
    CRISPR, Luciferase, Synthetic Biology, and Unspec…
    Expression
    Mammalian
    Available Since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    197265-plasmid-map-sequence-id-389723

    Enlarge

  5. pINSPECT_5'-xrRNA_NLuc_3'-XAP1

    Plasmid
    #197264
    Purpose
    Cloning Backbone for enhanced INSPECT expression (5' xrRNA + 3' XAP1). Encodes for intronic IRES-driven NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via BsaI.
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676
    Type
    Empty backbone
    Use
    CRISPR, Luciferase, Synthetic Biology, and Unspec…
    Expression
    Mammalian
    Available Since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    197264-plasmid-map-sequence-id-389660

    Enlarge

  6. pMX-53BP1(1-1710) 3LA

    Plasmid
    #185698
    Purpose
    Mammalian Expression, Retroviral vector that encodes a 3LA mutant of 53BP1 that disrupts three RIF1-binding motifs and abolishes the 53BP1-dependent recruitment of RIF1 to DNA double-strand breaks.
    Depositor
    Daniel Durocher
    Article
    Setiaputra et al Mol Cell. 2022 Apr 7;82(7):1359-1371.e9.
    Insert
    53BP1(1-1710) 3LA (TP53BP1 Human)
    Use
    Retroviral
    Tags
    Flag tag, HA tag, and tetracysteine tag
    Expression
    Mammalian
    Mutation
    L175A, L517A, and L693A
    Promoter
    SV40
    Available Since
    July 7, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    185698-plasmid-map-sequence-id-365946

    Enlarge

  7. pMX-53BP1(1-1710) 3LA7A

    Plasmid
    #185707
    Purpose
    Mammalian Expression, Retroviral vector that encodes 7A and 3LA mutant of 53BP1. Prevents shieldin and RIF1 recruitment to sites of double-strand breaks and disrupts 53BP1 activity.
    Depositor
    Daniel Durocher
    Article
    Setiaputra et al Mol Cell. 2022 Apr 7;82(7):1359-1371.e9.
    Insert
    53BP1(1-1710) 3LA7A (TP53BP1 Human)
    Use
    Retroviral
    Tags
    Flag tag, HA tag, and tetracysteine tag
    Expression
    Mammalian
    Mutation
    T302A, S452A, S523A, S543A, S625A, S784A, and S89…
    Promoter
    SV40
    Available Since
    July 1, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    185707-plasmid-map-sequence-id-365947

    Enlarge

  8. pINSPECT:IL2_SigP:NLuc

    Plasmid
    #197266
    Purpose
    Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human IL2 locus (exon 3). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676
    Insert
    Interleukin-2 homology arms
    Use
    CRISPR, Luciferase, Synthetic Biology, and Unspec…
    Expression
    Mammalian
    Available Since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    197266-plasmid-map-sequence-id-389716

    Enlarge

  9. pINSPECT:NEAT1-v2_SigP:NLuc

    Plasmid
    #197268
    Purpose
    Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human NEAT1 locus (long isoform only). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676
    Insert
    NEAT1-v2 homology arms
    Use
    CRISPR, Luciferase, Synthetic Biology, and Unspec…
    Expression
    Mammalian
    Available Since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    197268-plasmid-map-sequence-id-389661

    Enlarge

  12. pINSPECT:NEAT1-total_SigP:NLuc

    Plasmid
    #197267
    Purpose
    Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human NEAT1 locus (both isoforms). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676
    Insert
    NEAT1-total homology arms
    Use
    CRISPR, Luciferase, Synthetic Biology, and Unspec…
    Expression
    Mammalian
    Available Since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    197267-plasmid-map-sequence-id-400583

    Enlarge

  15. pPL001-BBTK

    Plasmid
    #184751
    Purpose
    Big-IN positive control payload encoding EF1a-driven GFP-T2A-BSD. Harbors a backbone counterselectable TK cassette.
    Depositor
    Jef Boeke
    Article
    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118
    Insert
    pEF1a-eGFP-T2A-BSD-bGHpA
    Use
    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    184751-plasmid-map-sequence-id-365380

    Enlarge

  16. ENABLE® Gene Editing in planta toolkit

    Plasmid Kit
    #1000000270
    Purpose
    A streamlined set of molecular tools for constructing CRISPR gene-editing vectors in just two simple cloning steps.
    Depositor
    Kate Creasey Krainer
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector Type
    Plant Expression
    Editing Type
    CRISPR
    Cloning Type
    Golden Gate
    Available Since
    Nov. 18, 2025
    Availability
    Academic Institutions and Nonprofits only

  17. C-term AAV-SpCas9-VRQR-gRNA_entry (HES1477)

    Plasmid
    #242660
    Purpose
    CBh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpCas9-VRQR and pU6 SpCas9 gRNA entry cassette
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pAAV-pCbh-BPNLS-NpuC-SpCas9-VRQR-BPNLS-WPRE-bGH_PA-SpCas9_gRNA_entrycassette
    Use
    AAV and CRISPR
    Tags
    BPNLS
    Mutation
    VRQR mutations in SpCas9(S55R/D1135V/G1218R/R1335…
    Promoter
    CBh
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242660-plasmid-map-sequence-id-489092

    Enlarge

  20. TALE Toolbox

    Plasmid Kit
    #1000000019
    Purpose
    A complete set of plasmids, optimized for human expression, to build your own TALE nucleases (TALENs) and transcription factors (TALE-TFs) for genome editing and transcription modulation applications.
    Depositor
    Feng Zhang
    Application
    Genome Editing
    Vector Type
    Mammalian Expression
    Editing Type
    TALEN
    Available Since
    Dec. 16, 2011
    Availability
    Academic Institutions and Nonprofits only
Showing: 1941 - 1960 of 9218 results