We narrowed to 150,436 results for: ins

1. pFastbac-Dual-StrepII-BARD1

   Plasmid

   #137166

   Purpose

   Expresses human StrepII-tagged BARD1 for purification from baculovirus/insect cell system

   Depositor

   Andrew Deans

   Article

   Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

   Insert

   BARD1 (BARD1 Human)
   

   Tags

   StrepII

   Expression

   Insect

   Mutation

   fully synthetic, codon optimized for insect expre…

   Promoter

   p10

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. FCM101

   Plasmid

   #194298

   Purpose

   E. coli expression vector for monobody adaptor, FCM101, that bind to the Fc region of mouse IgG1 subclass. It contains a single Cys residue at the C-terminus for chemical conjugation, N-terminal His6

   Depositor

   Shohei Koide

   Article

   Albert et al Nat Commun. 2022 Oct 11;13(1):5998. doi:

   Insert

   FCM101
   

   Tags

   His tag, FLAG tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. His6-SMT3-SopB(C460S)

   Plasmid

   #183677

   Purpose

   Recombinant Salmonella Typhimurium SopB (catalytically inactive)

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium, Budding Yeast)
   

   Tags

   His6-S. cerevisiae SMT3(2-101)

   Expression

   Bacterial

   Mutation

   C460S

   Promoter

   T7

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LADL Bridge + Promoter Only Target

   Plasmid

   #127669

   Purpose

   Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter as well as 2 gRNAs targeting the Zfp462 promoter expressed from their individual hU6 promoters

   Depositor

   Jennifer Phillips-Cremins

   Article

   Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

   Insert

   CRY2-HA-2A-mCherry and gRNAs 115 and 117
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Promoter

   EF1a and hU6

   Available Since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. MAP4K3-GFP d344-873, K45E

   Plasmid

   #21735

   Depositor

   L. Miguel Martins

   Article

   Lam et al Proc Natl Acad Sci U S A. 2009 Jul 21;10

   Insert

   MAP4K3 (MAP4K3 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Deletion of the carboxy-terminus, amino-acids 344…

   Available Since

   June 3, 2010

   Availability

   Academic Institutions and Nonprofits only

6. Gfa104-hIRβ

   Plasmid

   #236227

   Purpose

   Expressing a truncated, constitutively active human insulin receptor (IRβ) in astrocyte

   Depositor

   Olivier Thibault

   Article

   Frazier et al Neurochem Res. 2019 Jan;44(1):269-280. d

   Insert

   human Insulin Receptor beta subunit (INSR Human)
   

   Use

   AAV

   Expression

   Bacterial

   Available Since

   May 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLX304_miRFP670nano-P2A-ERM-gCam6f

   Plasmid

   #214927

   Purpose

   Express miRFP670nano P2A ERM-gCaMP6f (fluorescent proteins and genetic calcium sensor, gCaMP6f for normalizing factor and sensing calcium ions at cytosol exposed to ERM, respectively)

   Depositor

   Xinnan Wang

   Article

   Bharat et al Cell Rep. 2023 Dec 26;42(12):113544. doi

   Insert

   miRFP670nano-P2A-ERM-gCam6f
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMVp

   Available Since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. EGFP-SopB(172-554)

   Plasmid

   #183664

   Purpose

   N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Amino acids 172-554

   Promoter

   CMV

   Available Since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EGFP-SopB(211-554)

   Plasmid

   #183665

   Purpose

   N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Amino acids 211-554

   Promoter

   CMV

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV6-AN-DDK_1-590 POLI

    Plasmid

    #131233

    Purpose

    Mammalian expression of DNA polymerase iota residues 1-590 with an N-terminal FLAG-tag

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    DNA polymerase iota (POLI Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains a 24 bp insertion within the Pol iota co…

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV6-AN-DDK_1-420 POLI

    Plasmid

    #131234

    Purpose

    Mammalian expression of DNA polymerase iota residues 1-420 with an N-terminal FLAG-tag

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    DNA polymerase iota (POLI Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains a 24 bp insertion within the Pol iota co…

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV 3xFLAG-LC3B Q116P G120

    Plasmid

    #129289

    Purpose

    Expresses 3xFLAG-tagged deconjugation-resistant LC3B in mammalian cells

    Depositor

    Robin Ketteler

    Article

    Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Changed Glutamine 116 to Proline. Deleted amino a…

    Promoter

    CMV

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LADL Bridge + Target Zfp462-Klf4SE

    Plasmid

    #127668

    Purpose

    Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter; 2 gRNAs targeting the Zfp462 promoter and 2 gRNAs targeting the Klf4 SE expressed from their individual hU6 promoters

    Depositor

    Jennifer Phillips-Cremins

    Article

    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

    Insert

    CRY2-HA-2A-mCherry and gRNAs 129, 135, 115 and 117
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Promoter

    EF1a and hU6

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. BRI1 BRI1_pECIA14

    Plasmid

    #114886

    Purpose

    Prey vector BRI1 BRI1_pECIA14 should be used with bait vector BRI1 BRI1_pECIA2.

    Depositor

    Youssef Belkhadir

    Article

    Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

    Insert

    AT4G39400 (BRI1 Mustard Weed)
    

    Expression

    Insect

    Promoter

    Metallothionein (Copper-Inducible)

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. synapsin-hIRβ-mCherry

    Plasmid

    #228449

    Purpose

    Expressing a truncated, constitutively active human insulin receptor (IRβ) with mCherry in rat primary hippocampal neurons

    Depositor

    Olivier Thibault

    Article

    Frazier et al Neurochem Res. 2019 Jan;44(1):269-280. d

    Insert

    human Insulin Receptor beta subunit (INSR Human)
    

    Use

    AAV

    Promoter

    neuron-specific synapsin promote

    Available Since

    May 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAVS1-Hb9-CD14

    Plasmid

    #204344

    Purpose

    Knock-in vector to insert a motor neuron-specific MACS-sortable genetic reporter into the AAVS1 locus in human pluripotent stem cells. Allows isolation of human iPSC-derived motor neurons.

    Depositor

    Ivo Lieberam

    Article

    Harley et al Cell Rep. 2023 Dec 26;42(12):113509. doi

    Insert

    human CD14 (CD14 Human)
    

    Expression

    Mammalian

    Promoter

    mouse Hb9 (Mnx1) 9kb promoter fragment

    Available Since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDONR223 LC3B Q116P G120

    Plasmid

    #129291

    Purpose

    Gateway entry clone encoding human deconjugation-resistant LC3B

    Depositor

    Robin Ketteler

    Article

    Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Gateway entry vector / entry clone

    Mutation

    Changed Glutamine 116 to Proline. Deleted amino a…

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFBOH-MHL_DCAF11:1-546

    Plasmid

    #210907

    Purpose

    Insect expression of full-length human DCAF11. N-terminal 6X His tag with TEV cleavage.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    DCAF11:M1-Q546 (DCAF11 Human)
    

    Tags

    MHHHHHHSSGRENLYFQG

    Expression

    Insect

    Mutation

    R207H natural variant (VAR_020121)

    Promoter

    Polyhedrin

    Available Since

    March 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pFBOH-MHL_CDC20:161-477

    Plasmid

    #210943

    Purpose

    Insect expression of human CDC20 fragment S161 to P477. N-terminal 6X His tag with TEV cleavage.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    CDC20:S161-P477 (CDC20 Human)
    

    Tags

    MHHHHHHSSGRENLYFQG

    Expression

    Insect

    Mutation

    wild type

    Promoter

    Polyhedrin

    Available Since

    March 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pFBOH-MHL_EIF3B:127-814

    Plasmid

    #210909

    Purpose

    Insect expression of human EIF3B fragment D127 to E814. N-terminal 6X His tag with TEV cleavage.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    EIF3B:D127-E814 (EIF3B Human)
    

    Tags

    MHHHHHHSSGRENLYFQGMQ

    Expression

    Insect

    Mutation

    Y760C sequence conflict

    Promoter

    Polyhedrin

    Available Since

    March 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge