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Addgene

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We narrowed to 144,539 results for: ins

Showing: 1941 - 1960 of 144539 results

  2. PA-DAD-wt

    Plasmid
    #53095
    Purpose
    Expresses wild type PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light.
    Depositor
    Ronen Zaidel-Bar, Klaus Hahn
    Article
    Rao et al Cytoskeleton (Hoboken). 2013 Jul;70(7):3
    Insert
    LOV(wt)-6aa-DAD
    Use
    Tags
    6x His and mVenus
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    L47F and C49S in Venus
    Promoter
    Available since
    Aug. 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    53095-plasmid-map-sequence-id-119520

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  3. FCM101

    Plasmid
    #194298
    Purpose
    E. coli expression vector for monobody adaptor, FCM101, that bind to the Fc region of mouse IgG1 subclass. It contains a single Cys residue at the C-terminus for chemical conjugation, N-terminal His6
    Depositor
    Shohei Koide
    Article
    Albert et al Nat Commun. 2022 Oct 11;13(1):5998. doi:
    Insert
    FCM101
    Use
    Tags
    His tag, FLAG tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    194298-plasmid-map-sequence-id-381074

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  5. LADL Bridge + Promoter Only Target

    Plasmid
    #127669
    Purpose
    Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter as well as 2 gRNAs targeting the Zfp462 promoter expressed from their individual hU6 promoters
    Depositor
    Jennifer Phillips-Cremins
    Article
    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s
    Insert
    CRY2-HA-2A-mCherry and gRNAs 115 and 117
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and hU6
    Available since
    July 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    127669-plasmid-map-sequence-id-249167

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  8. pLX304_miRFP670nano-P2A-ERM-gCam6f

    Plasmid
    #214927
    Purpose
    Express miRFP670nano P2A ERM-gCaMP6f (fluorescent proteins and genetic calcium sensor, gCaMP6f for normalizing factor and sensing calcium ions at cytosol exposed to ERM, respectively)
    Depositor
    Xinnan Wang
    Article
    Bharat et al Cell Rep. 2023 Dec 26;42(12):113544. doi
    Insert
    miRFP670nano-P2A-ERM-gCam6f
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMVp
    Available since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    214927-plasmid-map-sequence-id-429502

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  14. LADL Bridge + Target Zfp462-Klf4SE

    Plasmid
    #127668
    Purpose
    Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter; 2 gRNAs targeting the Zfp462 promoter and 2 gRNAs targeting the Klf4 SE expressed from their individual hU6 promoters
    Depositor
    Jennifer Phillips-Cremins
    Article
    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s
    Insert
    CRY2-HA-2A-mCherry and gRNAs 129, 135, 115 and 117
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and hU6
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127668-plasmid-map-sequence-id-247046

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  17. AAVS1-Hb9-CD14

    Plasmid
    #204344
    Purpose
    Knock-in vector to insert a motor neuron-specific MACS-sortable genetic reporter into the AAVS1 locus in human pluripotent stem cells. Allows isolation of human iPSC-derived motor neurons.
    Depositor
    Ivo Lieberam
    Article
    Harley et al Cell Rep. 2023 Dec 26;42(12):113509. doi
    Insert
    human CD14 (CD14 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    mouse Hb9 (Mnx1) 9kb promoter fragment
    Available since
    Sept. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    204344-plasmid-map-sequence-id-407654

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  19. pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q23

    Plasmid
    #111723
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Article
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q23 (polyQ repeat)
    Promoter
    CMV and P10
    Available since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    111723-plasmid-map-sequence-id-220473

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  20. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q145

    Plasmid
    #111731
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Article
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q145 (polyQ repeat)
    Promoter
    CMV and P10
    Available since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    111731-plasmid-map-sequence-id-226895

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Showing: 1941 - 1960 of 144539 results