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Showing: 21 - 40 of 116 results

  1. pTUB1:YFP-mAID-3HA, DHFR-TS:HXGPRT

    Plasmid
    #87259
    Purpose
    YFP-mAID-3HA fusion driven by a minimal TUB1 promoter with an HXGdrug selectable marker. The mAID CDS is from Arabidopsis thaliana auxin-responsive protein IAA17E66-S133
    Depositor
    David Sibley
    Article
    Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d
    Inserts
    YFP-mAID-3HA
    HXGPRT
    Use
    Toxoplasma expression
    Tags
    3HA and mAID
    Expression
    Mutation
    Codon optimized for Toxoplasma gondii expression
    Promoter
    TgDHFR-TS 463 bp and TgTUB1 378 bp
    Availability
    Academic Institutions and Nonprofits only
    87259-plasmid-map-sequence-id-176555

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  5. pFloxed DHFR-TS*

    Plasmid
    #100605
    Purpose
    pUC19 with loxP-pDHFR-TS:DHFR-TS*(Pyr-R)-Stop-loxP-DHFR-TS 3' UTR
    Depositor
    David Sibley
    Insert
    Floxed TgDHFR-TS*
    Use
    Toxoplasma expression
    Tags
    Expression
    Mutation
    Pyrimethamine resistance
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    100605-plasmid-map-sequence-id-192861

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  6. pTUB1:OsTIR1-3FLAG, SAG1:CAT

    Plasmid
    #87258
    Purpose
    TIR1-3FLAG fusion driven by a TUB1 promoter with a CAT drug selectable marker. The TIR1 CDS from Oryza sativa was codon optimized for T. gondii expression.
    Depositor
    David Sibley
    Article
    Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d
    Inserts
    TIR1
    CAT
    Use
    Toxoplasma expression
    Tags
    3FLAG
    Expression
    Mutation
    Codon optimized for Toxoplasma gondii expression
    Promoter
    TgSAG1 361 bp and TgTUB1 2717 bp
    Availability
    Academic Institutions and Nonprofits only
    87258-plasmid-map-sequence-id-176556

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  7. pLinker-2xTy-HXGPRT-LoxP

    Plasmid
    #86664
    Purpose
    CRISPR tagging with 2xTy in Toxoplamsa gondii
    Depositor
    David Sibley
    Insert
    Linker-2xTy-HXGPRT 3'UTR
    Use
    Expression in toxoplasma gondii
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    86664-plasmid-map-sequence-id-193761

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  8. pUPRT::Floxed DHFR-TS*

    Plasmid
    #100606
    Purpose
    TgUPRT disruption by knock in with Floxed DHFR-TS*(Pyr-R) cassette with UPRT homology flanks.
    Depositor
    David Sibley
    Insert
    TgUPRT::Floxed TgDHFR-TS*
    Use
    Toxoplasma expression
    Tags
    Expression
    Mutation
    Pyrimethamine resistance
    Promoter
    pTgDHFR-TS
    Availability
    Academic Institutions and Nonprofits only
    100606-plasmid-map-sequence-id-192862

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  9. pSAG1::CAS9-U6::sgUPRT

    Plasmid
    #54467
    Purpose
    Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for disruption of genes by CRISPR.
    Depositor
    David Sibley
    Article
    Shen et al MBio. 2014 May 13;5(3). pii: e01114-14.
    Insert
    SAG1 5' UTR
    Use
    CRISPR; Toxoplasma expression
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    54467-plasmid-map-sequence-id-84808

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  13. pCAT-YFP-TetR

    Plasmid
    #59018
    Purpose
    Expresses a fusion of yellow fluorescent protein (YFP) to the N-terminus of the Escherichia coli Tn10 tet repressor (TetR) driven by the alpha tubulin promoter for use in T. gondii
    Depositor
    David Sibley
    Article
    Etheridge et al Cell Host Microbe. 2014 May 14;15(5):537
    Insert
    YFP-TetR
    Use
    Toxoplasma expression
    Tags
    Expression
    Mutation
    Promoter
    alpha-tubulin
    Availability
    Academic Institutions and Nonprofits only
    59018-plasmid-map-sequence-id-137741

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Showing: 21 - 40 of 116 results