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BL21ΔABCF

Bacterial Strain

#102270

Purpose

ompA, lamB, ompC, ompF quadruple knockout strain for overproduction of outer membrane proteins

Depositor

Jack Leo

Article

Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

Bacterial Resistance

Tetracycline

Available since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only
C321.ΔA

Bacterial Strain

#48998

Purpose

Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted)

Depositor

George Church

Article

Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

Bacterial Resistance

Ampicillin

Species

Escherichia coli

Available since

Oct. 24, 2013

Availability

Academic Institutions and Nonprofits only
rEcoli XpS

Bacterial Strain

#192872

Purpose

MG1655-C321 -- All 321 UAG codons changed to UAA

Depositor

Jesse Rinehart

Article

Gassaway et al Nat Methods. 2022 Oct 24. pii: 10.1038/s

Bacterial Resistance

Bleocin (zeocin)

Species

Escherichia coli

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only
Syn61Δ3(ev5) ΔrecA (ev1)

Bacterial Strain

#189857

Purpose

Laboratory adapted, genomically recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU, prfA, and recA genes. Evolved for ~270 generations in LB at 37 °C.

Depositor

George Church

Article

Nyerges et al Nature. 2023 Mar;615(7953):720-727. doi:

Bacterial Resistance

Streptomycin

Species

Synthetic

Available since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only
BL21 ΔrecBCD

Bacterial Strain

#176581

Purpose

E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression.

Depositor

Chase Beisel, Jean-Loup Faulon

Article

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Bacterial Resistance

None

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only
V. natriegens NC7

Bacterial Strain

#215356

Purpose

Vibrio natriegens with genomically integrated natural competence regulator, TfoX. For zero-capital molecular biology applications.

Depositor

Buz Barstow

Bacterial Resistance

None

Available since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only
Ochre (rEcΔ2.ΔA.B3.tW*)

Bacterial Strain

#234622

Purpose

Genomically recoded bacteria for site-specific incorporation of two nonstandard amino acids into proteins

Depositor

Farren Isaacs

Article

Grome et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Bacterial Resistance

Hygromycin

Available since

May 16, 2025

Availability

Academic Institutions and Nonprofits only
C321.∆A.opt

Bacterial Strain

#87359

Purpose

This is C321.∆A with additional mutations engineered by Kuznetsov et al. to improve doubling time.

Depositor

George Church

Article

Kuznetsov et al Genome Biol. 2017 May 25;18(1):100. doi:

Bacterial Resistance

Gentamicin

Species

Synthetic

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only
EC1000

Bacterial Strain

#71852

Purpose

RepA+ MC1000, KmR , carrying a single copy of the pWV01 repA gene in the glgB gene; host for pOR128-based plasmids

Depositor

Jan Kok, Todd Klaenhammer

Article

Leenhouts et al Mol Gen Genet. 1996 Nov 27;253(1-2):217-

Bacterial Resistance

Kanamycin

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only
C321.ΔA.M9adapted

Bacterial Strain

#98568

Purpose

Recoded strain C321.ΔA adaptively evolved for 1000+ generations in M9 minimal media + 1% glucose and biotin

Depositor

George Church

Article

Wannier et al Proc Natl Acad Sci U S A. 2018 Mar 20;11

Bacterial Resistance

Ampicillin

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only
BL21∆serB

Bacterial Strain

#34929

Depositor

Dieter Söll, Jesse Rinehart

Article

Park et al Science. 2011 Aug 26;333(6046):1151-4.

Bacterial Resistance

None

Available since

Feb. 24, 2012

Availability

Academic Institutions and Nonprofits only
RF11

Bacterial Strain

#61961

Purpose

C43(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. metA gene deleted

Depositor

Robert Gennis, Toshio Iwasaki

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
C321.deltaA (Isaacs lab)

Bacterial Strain

#73581

Purpose

Genomically recoded organism (E. coli C321.A, CP006698.1, GI:54981157). This strain is different than Addgene #68306 as it still retains the serB gene

Depositor

Farren Isaacs

Article

Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

Bacterial Resistance

Ampicillin

Available since

March 4, 2016

Availability

Academic Institutions and Nonprofits only
RHO3

Bacterial Strain

#124700

Purpose

E. coli mobilizer strain that facilitates conjugation of mobilizable plasmids by using metabolic counter-selection against the donor strain.

Depositor

Herbert Schweizer

Article

Lopez et al Appl Environ Microbiol. 2009 Oct;75(20):

Bacterial Resistance

Dap (400 µg/ml)

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only
MG1655 ΔendA ΔrecA

Bacterial Strain

#37853

Depositor

Kristala Prather

Article

Leonard et al Proc Natl Acad Sci U S A. 2010 Aug 3;107

Bacterial Resistance

None

Available since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only
sAJM.1504

Bacterial Strain

#108251

Purpose

Marionette-Clo. Marionette cluster inserted in the direction of leading strand replication, between 3,957,594 and 3,959,211 in E. coli DH10B

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Bacterial Resistance

Chloramphenicol

Available since

June 29, 2018

Availability

Academic Institutions and Nonprofits only
eScaf

Bacterial Strain

#220921

Purpose

cssDNA production strain

Depositor

Shawn Douglas

Article

Shen et al Nucleic Acids Res. 2024 Apr 24;52(7):409

Bacterial Resistance

None

Species

Escherichia coli

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only
TB205

Bacterial Strain

#230034

Purpose

Fluorescently labelled (mCherry) E. coli

Depositor

Calin Guet, Tobias Bergmiller

Article

Bergmiller et al Science. 2017 Apr 21;356(6335):311-315.

Bacterial Resistance

None

Available since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only
BL21(DE3) ΔserC

Bacterial Strain

#197656

Purpose

Derivative of BL21(DE3) with the serC gene knocked out. This strain is used for expressing proteins containing site-specific non-hydrolyzable phosphoserine.

Depositor

Ryan Mehl, Richard Cooley

Article

Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

Bacterial Resistance

None

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only
bMS.346

Bacterial Strain

#220588

Purpose

Increase editing efficiency of retron and oligo recombineering

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Bacterial Resistance

None

Available since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

Showing: 21 - 40 of 412 results

Results per page:

Sort results by:

BL21ΔABCF

C321.ΔA

rEcoli XpS

Syn61Δ3(ev5) ΔrecA (ev1)

BL21 ΔrecBCD

V. natriegens NC7

Ochre (rEcΔ2.ΔA.B3.tW*)

C321.∆A.opt

EC1000

C321.ΔA.M9adapted

BL21∆serB

RF11

C321.deltaA (Isaacs lab)

RHO3

MG1655 ΔendA ΔrecA

sAJM.1504

eScaf

TB205

BL21(DE3) ΔserC

bMS.346

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