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BL21(DE3) ΔserC

Bacterial Strain

#197656

Purpose

Derivative of BL21(DE3) with the serC gene knocked out. This strain is used for expressing proteins containing site-specific non-hydrolyzable phosphoserine.

Depositor

Ryan Mehl, Richard Cooley

Article

Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

Bacterial Resistance

None

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only
E. coli MEV20

Bacterial Strain

#197113

Purpose

E. coli BL21(DE3) derivative for diterpenoid production with genome-integrated yeast MEV pathway, redox enzyme array, and Marionette cluster

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Bacterial Resistance

None

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only
E. coli MEV15

Bacterial Strain

#197112

Purpose

E. coli BL21(DE3) derivative for sesquiterpenoid production with genome-integrated yeast MEV pathway, redox enzyme array, and Marionette cluster

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Bacterial Resistance

None

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only
RF15

Bacterial Strain

#102799

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB trpA trpB glyA serB genes deleted.

Depositor

Robert Gennis, Toshio Iwasaki

Article

Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

Bacterial Resistance

None

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only
RF16

Bacterial Strain

#102800

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB trpA trpB glyA serB cysE genes deleted.

Depositor

Robert Gennis, Toshio Iwasaki

Article

Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

Bacterial Resistance

None

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only
EC1000

Bacterial Strain

#71852

Purpose

RepA+ MC1000, KmR , carrying a single copy of the pWV01 repA gene in the glgB gene; host for pOR128-based plasmids

Depositor

Todd Klaenhammer, Jan Kok

Article

Leenhouts et al Mol Gen Genet. 1996 Nov 27;253(1-2):217-

Bacterial Resistance

Kanamycin

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only
MG1655 ΔendA ΔrecA (DE3)

Bacterial Strain

#37854

Depositor

Kristala Prather

Article

Tseng et al Microb Cell Fact. 2010 Nov 27;9:96.

Bacterial Resistance

None

Available since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only
DH10B-M.Osp807II

Bacterial Strain

#114267

Purpose

The strain expresses M.Osp807II methylase and zeocin selection marker from the arsB locus.

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Bacterial Resistance

Bleocin (zeocin)

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only
DH10B-M.XmnI

Bacterial Strain

#114269

Purpose

The strain expresses M.XmnI methylase and zeocin selection marker from the arsB locus.

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Bacterial Resistance

Bleocin (zeocin)

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only
DH10B-M2.NmeMC58II

Bacterial Strain

#114268

Purpose

The strain expresses M2.NmeMC58II methylase and zeocin selection marker from the arsB locus.

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Bacterial Resistance

Bleocin (zeocin)

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only
DHIP1

Bacterial Strain

#226851

Purpose

Expresses HIP1 methyltransferase to methylate plasmids for delivery to cyanobacteria

Depositor

David Nielsen

Article

Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

Bacterial Resistance

Streptomycin

Species

Escherichia coli

Available since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only
MCJ.559

Bacterial Strain

#69493

Purpose

Strain with recoded TAG and genomic deletions for improved incorporation

Depositor

Michael Jewett

Article

Hong et al Chembiochem. 2015 Mar 23;16(5):844-53. d

Bacterial Resistance

Ampicillin

Species

Eschericia coli

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only
rEc.13.delA

Bacterial Strain

#69495

Purpose

Recoded strain from MG1655, prfA deleted

Depositor

Michael Jewett

Article

Hong et al ACS Synth Biol. 2014 Jun 20;3(6):398-409

Bacterial Resistance

Ampicillin

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only
rEc.13

Bacterial Strain

#69494

Purpose

Recoded strain from MG1655 with prfA

Depositor

Michael Jewett

Article

Hong et al ACS Synth Biol. 2014 Jun 20;3(6):398-409

Bacterial Resistance

Ampicillin

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only
ATMW-BL21

Bacterial Strain

#218769

Purpose

E. coli strain, genotype: pUltraG ScW40CCA BL21 trpT::gentR trpS::zeoR

Depositor

Abhishek Chatterjee

Article

Ficaretta et al J Mol Biol. 2022 Apr 30;434(8):167304. d

Bacterial Resistance

Spectinomycin (50 ug/ml), gentamycin (10 ug/ml)

Species

Escherichia coli

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only
BL21(DE3)-R3-pRARE2

Bacterial Strain

#26242

Purpose

Phage-resistant derivative of BL21(DE3), which has been transformed with plasmid pRARE2, which carries seven rare-codon tRNA genes.

Depositor

Opher Gileadi

Article

Savitsky et al J Struct Biol. 2010 Jun 10. ():.

Bacterial Resistance

Chloramphenicol

Available since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only
E. coli BL21 (DE3) ΔEntD

Bacterial Strain

#192874

Purpose

Deletion of native E. coli EntD, a 4'-PPTase required for native enterobactin synthesis. Allows expression of NRPS carrier proteins that do not harbor a phosphopantetheinyl group.

Depositor

Christophe Guilhot, Christian Chalut

Article

Chalut et al Proc Natl Acad Sci U S A. 2006 May 30;10

Bacterial Resistance

None

Species

Escherichia coli

Available since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only
BM28 ΔlysU

Bacterial Strain

#201048

Purpose

MG1655-derived Escherichia coli isolate obtained from adaptive laboratory evolution to increasing incubation temperatures.

Depositor

Jeannette Winter

Article

McGuire et al BMC Genomics. 2023 Mar 27;24(1):154. doi

Bacterial Resistance

Kanamycin

Available since

May 18, 2023

Availability

Academic Institutions and Nonprofits only
Syn61

Bacterial Strain

#174513

Purpose

Recoded E. coli strain without TCG, TCA, or TAG codons in all open reading frames. Full sequence - GenBank: CP040347.1

Depositor

Jason W Chin

Article

Fredens et al Nature. 2019 May;569(7757):514-518. doi:

Bacterial Resistance

Streptomycin

Available since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only
RF21

Bacterial Strain

#102803

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB ilvE avtA yfbQ(alaA) yfdZ(alaC) genes deleted. (yfbQ also called alaA; yfdZ also called alaC)

Depositor

Robert Gennis, Toshio Iwasaki

Article

Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

Bacterial Resistance

None

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

Showing: 21 - 40 of 90 results

Results per page:

Sort results by:

BL21(DE3) ΔserC

E. coli MEV20

E. coli MEV15

RF15

RF16

EC1000

MG1655 ΔendA ΔrecA (DE3)

DH10B-M.Osp807II

DH10B-M.XmnI

DH10B-M2.NmeMC58II

DHIP1

MCJ.559

rEc.13.delA

rEc.13

ATMW-BL21

BL21(DE3)-R3-pRARE2

E. coli BL21 (DE3) ΔEntD

BM28 ΔlysU

Syn61

RF21

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