161,704 results

1. pSMPP-mCherry-hTRIM21

   Plasmid

   #104972

   Purpose

   Lentiviral Transfer Plasmid with mCherry-tagged human TRIM21

   Depositor

   Leo James

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   Tripartite Motif containing 21 (TRIM21 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Feb. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. DENV2 NS2B-NS3 protease: Co-expression of DENV-2 NS2B and NS3

   Plasmid

   #204808

   Purpose

   Bacterial co-expression of Dengue2 NS2B and NS3 proteins

   Depositor

   Frank von Delft, ASAP Discovery

   Insert

   Dengue2 NS2B (POLY Dengue2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 18, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pSpCas9(BB)-2A-mCherry

   Plasmid

   #197420

   Purpose

   Cas9 from S. pyogenes with 2A-mCherry, and cloning backbone for sgRNA

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. eGFP-proα2(I)

   Plasmid

   #119826

   Purpose

   Expresses mouse Type I procollagen α2 chain (Col1a2) with GFP between signal sequence and exon 6

   Depositor

   Sergey Leikin

   Article

   Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

   Insert

   Type I procollagen α2 chain (Col1a2 Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Col1a2 exons 2-5 replaced by fluorescent tag

   Promoter

   CMV

   Available since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. V247 pCEP 4HA B56gamma1

   Plasmid

   #14534

   Depositor

   David Virshup

   Article

   Seeling et al Science. 1999 Mar 26. 283(5410):2089-91.

   Insert

   PPP2R5C B56 gamma1 regulatory subunit of PP2A (PPP2R5C Human)
   

   Use

   Tags

   4 HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. mTAGBFP2 WC visual barcode

   Plasmid

   #158666

   Purpose

   tagging clonal populations with whole cell mTAGBFP2 fluorescent protein

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Insert

   mTAGBFP2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCW57.1_blasti

   Plasmid

   #194067

   Purpose

   3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR221-JUN

   Plasmid

   #79512

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Human JUN CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   JUN (JUN Human)
   

   Use

   Multisite gateway entry clone

   Tags

   HA Tag

   Expression

   Mutation

   Promoter

   no Promoter

   Available since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 Flag Runx1 FL (P#1790)

   Plasmid

   #14585

   Depositor

   Matija Peterlin

   Article

   Jiang et al Mol Cell Biol. 2005 Dec . 25(24):10675-8

   Insert

   Runx1 (Runx1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. 6xHis-TagRFP-T_A1aY1

    Plasmid

    #158754

    Purpose

    Expression of 6x His tagged TagRFP-T_A1aY1 in mammalian cells for detection of tyrosinated microtubules.

    Depositor

    Minhajuddin Sirajuddin

    Article

    Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

    Insert

    6xHistidine_TagRFP-T_A1aY1
    

    Use

    Tags

    6x-Histidine tag and TagRFP-T

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLP-PIGA (pLP140)

    Plasmid

    #168461

    Purpose

    Big-IN Landing Pad plasmid encoding pEF1a-driven mScarlet-P2A-CreERT2-P2A-PuorR-P2A-hmPIGA expression cassette flanked by heterotypic LoxM/LoxP sites and BsaI homology arms cloning sites.

    Depositor

    Matthew Maurano, Jef Boeke

    Article

    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

    Type

    Empty backbone

    Use

    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmyc-TNRC6B

    Plasmid

    #10978

    Depositor

    Thomas Tuschl

    Article

    Meister et al Curr Biol. 2005 Nov 9. ():.

    Insert

    TNRC6B (TNRC6B Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRTC2-puro

    Plasmid

    #190567

    Purpose

    Expresses the full-length human L1 (L1.3) retrotransposon and an engineered neomycin retrotransposition reporter to monitor retrotransposition efficiency in cultured mammalian cells.

    Depositor

    Anthony Furano

    Article

    Cook et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    L1 (L1.3) with reporter cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLM-mCerulean-cMyc

    Plasmid

    #23244

    Depositor

    Michel Sadelain

    Article

    Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

    Insert

    mCerulean-2A-MYC (MYC synthetic fluorescent protein, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. HDM_RSV_A_1982_F

    Plasmid

    #237355

    Purpose

    Expression plasmid for RSV A F from 1982

    Depositor

    Jesse Bloom

    Article

    Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

    Insert

    RSV A F from 1982 human codon optimized from Genbank Accesion KJ723472
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. HDM_RSV_A_2020_F

    Plasmid

    #237356

    Purpose

    Expression plasmid for RSV A F from 2020

    Depositor

    Jesse Bloom

    Article

    Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

    Insert

    RSV A F from 2020 human codon optimized from Genbank Accesion PP495954
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEGFP_TOMM20_sfCherry2(11)

    Plasmid

    #83033

    Purpose

    Expresses sfCherry2(11) tagged TOMM20 (human) in mammalian cells cells

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    TOMM20_sfCherry+11
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQdC12a

    Plasmid

    #191356

    Purpose

    Clostridium CRISPRi plasmid with TetR-repressible dCas12a (LbCpf1) expression

    Depositor

    Andrew Tolonen

    Article

    Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

    Inserts

    PGusA2-TetO2/1-dCas12a (LbCpf1)
    miniPthl-tetR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PGus2A-TetO2/1 and miniPthl

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET29a-human histone 4-in-One

    Plasmid

    #223237

    Purpose

    Polycistronic bacterial coexpression construct for human His-Sumo-H2A, H2B, H3, and Dual-StrepII-H4

    Depositor

    Weixing Zhao

    Inserts

    His6-VSVG-Histone H2A
    Histone H2B
    Histone H3
    Histone H4-TEV-His6-Dual StrepII
    

    Use

    Tags

    Dual StrepII, His6, TEV protease site, and VSVG

    Expression

    Bacterial

    Mutation

    A103G

    Promoter

    T7

    Available since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. CMV-ER-LAR-GECO1

    Plasmid

    #61244

    Purpose

    Expresses LAR-GECO1 in the endoplasmic reticulum in mammalian cells

    Depositor

    Robert Campbell

    Article

    Wu et al Biochem J. 2014 Nov 15;464(1):13-22. doi

    Insert

    LAR-GECO1
    

    Use

    Tags

    ER-retention sequence: KDEL and ER-targeting sequ…

    Expression

    Mammalian

    Mutation

    Substitutions relative to R-GECO1: V51W/I113V/N35…

    Promoter

    CMV

    Available since

    March 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAAV-R-Creon shRNA[Control]

    Plasmid

    #180775

    Purpose

    This vector simultaneously expresses mCherry protein and shRNA in a Cre-dependent manner.

    Depositor

    Eun Mi Hwang

    Article

    Kim et al J Neurosci Methods. 2021 Dec 21:109452.

    Type

    Empty backbone

    Use

    AAV, Cre/Lox, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. MSCV-N LMP1

    Plasmid

    #37962

    Depositor

    Karl Munger

    Article

    Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

    Insert

    LMP1 (LMP-1 H. Herpesvirus 4 (EBV))
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    PKG

    Available since

    Aug. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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23. pFastBac-Dual-M2

    Plasmid

    #135584

    Purpose

    High eukaryotic dual protein expression shuttle vector

    Depositor

    Xiao-Wen Cheng

    Article

    Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

    Type

    Empty backbone

    Use

    Transfer vector

    Tags

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin of AcMNPV

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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24. MAC_N_KLF9

    Plasmid

    #167774

    Purpose

    Expresses KLF9 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    KLF9 (KLF9 Human)
    

    Use

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. pOpen-phi29pol

    Plasmid

    #165573

    Purpose

    DNA polymerase responsible for protein-primed viral DNA replication by strand displacement with high processivity and fidelity. Possesses three enzymatic activities: DNA synthesis (polymerase), primer terminal protein (TP) deoxynucleotidylation, and 3' to 5' exonuclease activity.

    Depositor

    Drew Endy, Jennifer Molloy, FreeGenes Project

    Insert

    phi29 DNA Polymerase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. eGFP STAT1 WT

    Plasmid

    #12301

    Depositor

    Alan Perantoni

    Article

    Timofeeva et al Oncogene. 2006 Jun 26. ():.

    Insert

    STAT1 (STAT1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pNHEJ-recX-sacB

    Plasmid

    #158718

    Purpose

    CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum and recX.

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

    Insert

    recX
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pmyc

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Human PNP his-tagged

    Plasmid

    #64076

    Purpose

    Expression of human Purine Nucleoside Phosphorylase

    Depositor

    Vern Schramm

    Article

    Lewandowicz et al J Biol Chem. 2003 Aug 22;278(34):31465-8

    Insert

    Purine Nucleoside Phosphorylase (PNP Human)
    

    Use

    Tags

    Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pMIR-FLAG-MLL-AF9

    Plasmid

    #71444

    Purpose

    Retroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-dsRed Express2

    Depositor

    Daisuke Nakada

    Article

    Saito et al Cell Stem Cell. 2015 Sep 29. pii: S1934-

    Insert

    MLL-AF9 (KMT2A Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. NeonGreen-C1-ORP6

    Plasmid

    #220072

    Purpose

    overexpresses ORP6 with a neonGreen fluorophore

    Depositor

    Gerry Hammond

    Article

    Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

    Insert

    OSBPL6 (OSBPL6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge