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27,467 results

Showing: 8381 - 8400 of 27467 results

  1. pFX-EGFP-EEA1

    Plasmid
    #174451
    Purpose
    Express EGFP with early endosome-targeting sequence in mammalian cells
    Depositor
    Yusuke Ohba
    Publication
    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP with early endosome-targeting sequence
    Tags
    early endosomal antigen 1 (EEA1)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    174451-plasmid-map-sequence-id-354863

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  4. pSmart-EF1a-Nla-cas3

    Plasmid
    #178881
    Purpose
    Expression of human codon-optimized NlaCas3 with C terminal NLS and HA tag
    Depositor
    Yan Zhang
    Publication
    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EF1a-NlaCas3c-bGH polyA
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Jan. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    178881-plasmid-map-sequence-id-351754

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  5. pYL-kasOp-FnCas12a2

    Plasmid
    #177874
    Purpose
    Expresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.
    Depositor
    Yunzi Luo
    Publication
    Zhang et al Front Bioeng Biotechnol. 2020 Jun 30;8:7
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Streptomyces codon optimized FnCas12a
    Use
    CRISPR
    Promoter
    kasOp*
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    177874-plasmid-map-sequence-id-352015

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  12. 10TALE_Pmin_fLuc

    Plasmid
    #160663
    Purpose
    Expression of the firefly luciferase reporter upon binding of a transcriptional activator to target sites. The plasmid contains 10 copies of target sites for the TALEN1257 DNA-binding domain
    Depositor
    Roman Jerala
    Publication
    Mesko et al ACS Synth Biol. 2020 Aug 21;9(8):2055-20
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    10TALE_Pmin_fLuc
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    minimal
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160663-plasmid-map-sequence-id-345378

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  14. DDX41-GFP

    Plasmid
    #175494
    Purpose
    Total RNA was isolated from TF-1 cells and converted to cDNA using SuperScript III (Invitrogen). The full DDX41 coding sequence was cloned into pcDNA3.1 (Invitrogen) with an EGFP c-terminal insert.
    Depositor
    Evan Braunstein
    Publication
    Li et al Haematologica. 2016 Jun;101(6):e228-31.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DDX41 (DDX41 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    175494-plasmid-map-sequence-id-345045

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  15. pSF4 TetCMV ATF4(5'UTR)-SunTag-Renilla-FKBP-Stop-24xMS2v5-CTE-polyA

    Plasmid
    #164615
    Purpose
    Expresses ATF4(5'UTR)-SunTag-Renilla mRNA reporter under tetracycline-inducible promoter. SunTag and Renilla luciferase are in frame with the main start codon of ATF4.
    Depositor
    Jeffrey Chao
    Publication
    Mateju et al Cell. 2020 Dec 23;183(7):1801-1812.e13.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Renilla luciferase
    Tags
    24xMS2v5 (3'UTR) and SunTag
    Expression
    Bacterial and Mammalian
    Promoter
    TetCMV
    Available since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    164615-plasmid-map-sequence-id-325006

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  16. pX330 _CRISPIE donor B8

    Plasmid
    #172849
    Purpose
    DRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B8 (Zhong et al, eLife 2021), mEGFP translational phase (1-1), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).
    Depositor
    Haining Zhong
    Publication
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DRS-2 sgRNA and CRISPIE designer exon (phase 1-1) encoding mEGFP
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    172849-plasmid-map-sequence-id-340928

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  19. SauCas9 PE2*

    Plasmid
    #169851
    Purpose
    Mammalian Expression, sauCas9 based prime editor
    Depositor
    Scot Wolfe
    Publication
    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SaCas9-N580A-M-MLV
    Tags
    bpSV40 NLS and SV40 NLS and cmyc-NLS and bpSV40 N…
    Expression
    Mammalian
    Promoter
    cmv
    Available since
    June 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    169851-plasmid-map-sequence-id-332821

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  20. pcDNA3.1-GGamma5-GFP2

    Plasmid
    #166777
    Purpose
    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GGamma5-GFP2 (GNG5 Synthetic, Human)
    Expression
    Mammalian
    Mutation
    Contains an N-terminal GFP2 and GSAG linker
    Promoter
    CMV
    Available since
    May 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    166777-plasmid-map-sequence-id-408989

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Showing: 8381 - 8400 of 27467 results