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27,466 results

Showing: 11201 - 11220 of 27466 results

  1. mGFP-hNET

    Plasmid
    #193364
    Purpose
    expression of human norpinephrine transporter tagged with monomeric EGFP at the N-terminus in mammalian cells
    Depositor
    Harald Sitte
    Publication
    Luethi et al Commun Biol. 2022 Nov 17;5(1):1259. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human Norepinephrine Transporter (SLC6A2 Human)
    Tags
    monomeric EGFP (mEGFP)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193364-plasmid-map-sequence-id-381453

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  5. pAK_DR30_CasRx_mCherry

    Plasmid
    #134844
    Purpose
    All in one overexpression of CasRx and guideRNA
    Depositor
    Michael Yaffe
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RfxCas13d
    Use
    CRISPR
    Tags
    T2A mCherry
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Nov. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    134844-plasmid-map-sequence-id-266704

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  7. ER2-mhYFP

    Plasmid
    #186528
    Purpose
    Localization and retention of monomeric hyperfolder YFP fluorescent protein in the ER; improved targeting efficiency
    Depositor
    Gregory Petsko
    Publication
    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Monomeric hyperfolder YFP
    Tags
    Calreticulin and KDEL
    Expression
    Mammalian
    Mutation
    hfYFP-S147P/L195M/V206K
    Promoter
    CMV
    Available since
    Nov. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    186528-plasmid-map-sequence-id-373452

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  8. EK0493 SFFV-mCherry-SGc(GAPDH.s1)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid
    #191156
    Purpose
    Expression of a sensor for GAPDH 3' UTR in mammalian cells; mCherry marker, EGFP output, three MS2 sequences in 3' UTR
    Depositor
    Xiaojing Gao
    Publication
    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry:GAPDH.s1:EGFP-3xMS2
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    SFFV
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    191156-plasmid-map-sequence-id-375490

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  9. EK0438 SFFV-mCherry-SGc(s70587)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid
    #191163
    Purpose
    Expression of a sensor for synthetic sequence 1 (EK0208) with three MS2 sequences in the 3' UTR in mammalian cells. Compared to EK0285, also has additional restriction sites around the sensor sequence. Recommended for the basis for cloning new sensors for use with MCP-ADAR(DD). HindIII & MluI can be used to swap out marker (mCherry). (KpnI or BamHI) and (BshTI or BcuI) can be used to swap out sensor sequence (s70587). XhoI and ApaI can be used to swap out output (EGFP).
    Depositor
    Xiaojing Gao
    Publication
    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry:s70587:EGFP-3xMS2
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    SFFV
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    191163-plasmid-map-sequence-id-375409

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  10. EK0409 SFFV-mCherry-SGc(s70587)cSG-EGFP (FLP-IN)

    Plasmid
    #191164
    Purpose
    Similar to EK0285 but has additional restriction sites around the sensor sequence. Recommended for cloning new sensors when the presence of MS2 sequences are not relevant (use EK0438 if MS2 sequences
    Depositor
    Xiaojing Gao
    Publication
    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry:s70587:EGFP (additional cutsites)
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    SFFV
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    191164-plasmid-map-sequence-id-375473

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  13. mCherry-p62-Silent_Mutation_WT

    Plasmid
    #187986
    Purpose
    human p62 with silent mutation for sip62 #5 (Thermo Scientific). Internal reference: SMC516
    Depositor
    Sascha Martens
    Publication
    Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    p62 (NUP62 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    silent mutation for resistance to sip62 #5
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    187986-plasmid-map-sequence-id-374441

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  14. pJEC819

    Plasmid
    #190815
    Purpose
    CRISPRa backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.
    Depositor
    James Chappell
    Publication
    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-XTEN-rpoA
    Use
    CRISPR and Synthetic Biology
    Tags
    XTEN (linker), followed by the N-terminal domain …
    Promoter
    SP30
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    190815-plasmid-map-sequence-id-373717

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  16. pCAGGS SARS-CoV-2 BA.4.6 Spike

    Plasmid
    #190013
    Purpose
    Expresses codon-optimized full length SARS-CoV-2 BA.4.6 Spike
    Depositor
    Marceline Côté
    Publication
    Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 BA.4.6 Spike (S )
    Expression
    Mammalian
    Mutation
    Contains the following mutations: T19I, LPPA24S, …
    Promoter
    chicken β-actin promoter, CMV enhancer
    Available since
    Sept. 7, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    190013-plasmid-map-sequence-id-371149

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Showing: 11201 - 11220 of 27466 results