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27,006 results

Showing: 5121 - 5140 of 27006 results

  5. pRK7-HA-MSK2

    Plasmid
    #8994
    Depositor
    John Blenis
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MSK2 (RPS6KA4 Human)
    Tags
    HA
    Expression
    Mammalian
    Available since
    Sept. 2, 2005
    Availability
    Academic Institutions and Nonprofits only

  6. pcDNA3/td5StayGold(c4)=GianCreg

    Plasmid
    #212022
    Purpose
    For labeling the Golgi apparatus. c4 is the appendage for N-terminal tagging with td5StayGold. ‘GianCreg’ is an abbreviation for giantin C-terminal region. ‘=’ denotes a linker [(GGGGS)3].
    Depositor
    Atsushi Miyawaki
    Publication
    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GianCreg (GOLGB1 Human)
    Tags
    td5StayGold(c4)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    212022-plasmid-map-sequence-id-419221

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  7. pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet3

    Plasmid
    #189752
    Purpose
    Produces an mTurquoise-antiFRET-mScarlet3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet3 FPs for ratiometric analysis
    Depositor
    Dorus Gadella
    Publication
    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mTurquoise2_spatial-linker-P2A_mScarlet3
    Tags
    6xHis
    Expression
    Bacterial and Mammalian
    Promoter
    CMV & Rha
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    189752-plasmid-map-sequence-id-375290

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  11. pcDNA3.1-hfYFP

    Plasmid
    #186523
    Purpose
    Hyperfolder YFP fluorescent protein: cytosolic expression in mammalian cells
    Depositor
    Gregory Petsko
    Publication
    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Hyperfolder YFP
    Expression
    Mammalian
    Mutation
    mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…
    Promoter
    CMV
    Available since
    Nov. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    186523-plasmid-map-sequence-id-373443

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  12. pCBh_NLS_dCas13X_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid
    #191796
    Purpose
    vector for encoding a human codon-optimized dead Cas13X (dCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter
    Depositor
    Huawei Tong
    Publication
    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized dCas13X
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    R84A, H89A, R739A, R740A, H745A, H746A, H747A
    Promoter
    CBh, SV40, hU6
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    191796-plasmid-map-sequence-id-375578

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  13. pFX-Tom20-EGFP

    Plasmid
    #174186
    Purpose
    Express EGFP with mitochondria-targeting sequence in mammalian cells
    Depositor
    Yusuke Ohba
    Publication
    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP with mitchondria-targeting sequence
    Tags
    the mitochondria-targeting sequence of TOM20
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    174186-plasmid-map-sequence-id-354852

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  14. CAG FUCCI

    Plasmid
    #175266
    Purpose
    Bicistronic FUCCI construct driven by a CAG promoter. Contains I-SceI sites for transgenesis
    Depositor
    James Monaghan
    Publication
    Duerr et al Development. 2022 Mar 15;149(6). pii: 27
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FUCCI
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Sept. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    175266-plasmid-map-sequence-id-343477

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  15. G15-CASE

    Plasmid
    #168126
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein G15. Composed of the subunits G alpha 15 (GNA15) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Publication
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GNA15, GNB3, GNG9 (GNA15, GNB3, GNGT2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG9
    Expression
    Mammalian
    Mutation
    NLuc is inserted at E244/N245 within GNA15
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168126-plasmid-map-sequence-id-332871

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  17. pcDNA3.1-GGamma2-GFP2

    Plasmid
    #166774
    Purpose
    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GGamma2-GFP2 (GNG2 Human, Synthetic)
    Expression
    Mammalian
    Mutation
    Contains an N-terminal GFP2 and GSAG linker
    Promoter
    CMV
    Available since
    May 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    166774-plasmid-map-sequence-id-330993

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Showing: 5121 - 5140 of 27006 results