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27,006 results

Showing: 5821 - 5840 of 27006 results

  1. mito-mStayGold_pTwistCMVBetaGlobWPRENeo

    Plasmid
    #221898
    Purpose
    Expresses mStayGold targeted to the mitochondria
    Depositor
    Janelia Integrative Imaging
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cox8-mStayGold
    Tags
    Cox8
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    221898-plasmid-map-sequence-id-438069

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  2. TDP-REGv2(mScarlet-FLAG)#7 in pTwist-CMV backbone

    Plasmid
    #216152
    Purpose
    Expresses mScarlet in cells with TDP-43 loss-of-function. This plasmid has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells. It uses a cryptic exon. [Code 'A11'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Publication
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mScarlet with cryptic exon and C-terminal FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216152-plasmid-map-sequence-id-437762

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  3. pNeonGreen-C1-Nir1-LNS2

    Plasmid
    #220083
    Purpose
    High affinity PA biosensor with green fluorophore (also known as NeonGreen-PILS-Nir1)
    Depositor
    Gerry Hammond
    Publication
    Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PITPNM3 (PITPNM3 Human)
    Tags
    NeonGreen-GGSGGM
    Expression
    Mammalian
    Mutation
    amino acids 613-897
    Promoter
    CMV
    Available since
    June 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    220083-plasmid-map-sequence-id-434082

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  4. U6-5'+3' MS2-CircRNA

    Plasmid
    #213752
    Purpose
    For circular RNA-mediated prime editor using U6-5'+3' MS2-CircRNA in HEK293T cells
    Depositor
    Caixia Gao
    Publication
    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    5' ribozyme, 5' ligation sequences, 5' MS2, 3' MS2, 3' ligation sequences, 3' ribozyme
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Feb. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    213752-plasmid-map-sequence-id-422189

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  5. CMV-enAsCas12f

    Plasmid
    #204637
    Purpose
    Expression of enAsCas12f in mammalian cells
    Depositor
    Weixin Tang
    Publication
    Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    enAsCas12f
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    D196K, N199K, G276R, N328G, D364R
    Promoter
    CMV
    Available since
    Aug. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    204637-plasmid-map-sequence-id-403532

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  6. TRUPATH Triple Ga12

    Plasmid
    #196061
    Purpose
    Encodes a G alpha subunit (GNA12) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GAlpha12-RLuc8 (GNA12 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    196061-plasmid-map-sequence-id-385874

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  7. T7-VEE-OCT4-t2a-KLF4-e2a-SOX2-17-IRES-GLIS1

    Plasmid
    #193356
    Purpose
    VEE-OKS*iG (self-replicating RNA vector expressing human OCT4 + KLF4 + super-SOX + GLIS1 + PuroR) for generation of integration-free iPSCs
    Depositor
    Hans Schöler
    Publication
    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    OCT4-t2a-KLF4-e2a-SOX2-17-IRES-GLIS1 (KLF4, SOX2, POU5F1 Human)
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Expression
    Mammalian
    Mutation
    SOX2-17 is engineered highly cooperative chimeric…
    Available since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    193356-plasmid-map-sequence-id-379848

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  9. pPolI_SARS-CoV-2_NLuc

    Plasmid
    #183953
    Purpose
    Encodes SARS-CoV-2 minigenome with nano-luciferase reporter gene. Minigenome is under control of the Pol I promoter
    Depositor
    AJ te Velthuis
    Publication
    Vial et al bioRxiv 2021.06.28.450211
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 minigenome with Nano luciferase (N, ORF10 SARS coronavirus 2)
    Expression
    Mammalian
    Promoter
    Mammalian Pol I
    Available since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    183953-plasmid-map-sequence-id-365335

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  10. pCAGGS SARS-CoV-2 BA.1 Spike

    Plasmid
    #185452
    Purpose
    Expresses codon-optimized full length SARS-CoV-2 BA.1 Spike
    Depositor
    Marceline Côté
    Publication
    Chatterjee et al Cell Rep. 2022 Mar 1;38(9):110429. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 Spike BA.1 (S )
    Expression
    Mammalian
    Mutation
    Contains the following mutations: A67V, Δ69-70, T…
    Promoter
    chicken β-actin promoter, CMV enhancer
    Available since
    June 8, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    2 Articles
    185452-plasmid-map-sequence-id-363821

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  11. pICE-EGFP-FLAG-PLIN3

    Plasmid
    #161918
    Purpose
    Expresses human PLIN3 with a N-terminal GFP-FLAG tag. Confers Puromycin resistance. Inducible in T-REx cells.
    Depositor
    Sébastien Britton
    Publication
    Demange et al Elife. 2022 May 10;11. pii: 73913. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Perilipin-3 (PLIN3 Human)
    Tags
    GFP-FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    161918-plasmid-map-sequence-id-318840

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  13. pCMMP-MCS-IRES-eGFP-P2A-iCas9

    Plasmid
    #178533
    Purpose
    For genome editing: EGFP-inducible Caspase9 knock-in cassette which allows ablation of target cells
    Depositor
    Ryoji Yao
    Publication
    Okamoto et al STAR Protoc. 2021 Sep 16;2(4):100780. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    iCaspase9 (CASP9 Human)
    Expression
    Mammalian
    Mutation
    FKBP12 F36 to V Truncated Caspase9 (135-416; lack…
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    178533-plasmid-map-sequence-id-351918

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  15. TNFa-GFP

    Plasmid
    #163660
    Purpose
    Expresses GFP tagged TNFa in mammalian cells
    Depositor
    Lei Lu
    Publication
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    tumor necrosis factor (TNF Human)
    Tags
    GFP
    Expression
    Mammalian
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163660-plasmid-map-sequence-id-322791

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  17. CMV-dCas13X.1-REPAIRv2-SV40pA_CMV-mCherry-BGHpA_U6-BbsI-DR

    Plasmid
    #171382
    Purpose
    Expression vector for encoding a human codon-optimized dCas13X.1-REPAIRv2 driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.
    Depositor
    Hui Yang
    Publication
    Xu et al Nat Methods. 2021 May;18(5):499-506. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized dCas13X.1-REPAIRv2
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV, U6
    Available since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    171382-plasmid-map-sequence-id-337349

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Showing: 5821 - 5840 of 27006 results