30,881 results
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Plasmid#209872PurposeDimerization dependent fluorescent protein RA anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertddRFP A
TagsTargeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…ExpressionMammalianPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-EGFR-V5/HIS
Plasmid#201102Purposeexpression of human EGFR receptor tyrosine kinase in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman EGFR receptor tyrosine kinase, full length, wildtype (EGFR Human)
TagsV5/HisExpressionMammalianPromoterCMVAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDAC569
Plasmid#195241PurposeMammalian expression of Csm complex (RNase mut) from separate promoters; GFP backbone (Used for RNA binding/tethering)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3(RNase mut); FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; GFP
ExpressionMammalianAvailable sinceFeb. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-TDP-43
Plasmid#190093PurposeExpresses TDP-43 in mammalian cells. Note: EGFP and TDP-43 are expressed in different reading frames.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTAR DNA-Binding Protein 43 (TARDBP Human)
ExpressionMammalianAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-TargetBC-5xTAPE-1-pegRNA-InsertBC
Plasmid#183790PurposeA single construct out of the pool of plasmid for lineage recording experiment using DNA Ticker Tape.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsP2A-eGFP-TargetBC-5xTAPE-1
pegRNA-InsertBC
UseSynthetic BiologyMutationG>A in the gRNA scaffold- please see depositor…Available sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
B-gTEMP/pcDNA3
Plasmid#188447PurposeB-gTEMP for mammalian expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertB-gTEMP
ExpressionMammalianAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
HaloTag-sec61b
Plasmid#186959PurposeExpress sec61b connected with N-terminus HaloTagDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSec61 (SEC61B Human)
TagsHaloTagExpressionMammalianPromoterCMV PromoterAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-RIα-EGFP
Plasmid#181840PurposeGFP-tagged PKA RIα subunit for mammalian expression.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRIα-EGFP (PRKAR1A Human)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAGGS_cAMPFIRE-H
Plasmid#182285PurposeMammalian expression of the cAMP sensor cAMPFIRE-H under a CAG promotor w/ WPRE translation enhancer.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcAMPFIRE-H (cAMP sensor)
ExpressionMammalianAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
px330_Rosa_sgRNA
Plasmid#97007PurposeExpresses Cas9 and Rosa26 locus specific sgRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRosa26 sgRNA
UseCRISPRExpressionMammalianPromoterU6Available sinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFX-mCherry-EEA1
Plasmid#174452PurposeExpress mCherry with early endosome-targeting sequence in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry with early endosome-targeting sequence
Tagsearly endosomal antigen 1 (EEA1)ExpressionMammalianPromoterCMVAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Rogi1-CMV-LAMB3-T2A-GFP-bGH
Plasmid#179864PurposeTargets Rogi1 genomic region with HDR and expresses LAMB3-T2A-GFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLAMB3-T2A-GFP
Available sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
MAC_N_IRF1
Plasmid#167760PurposeExpresses IRF1 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertIRF1 (IRF1 Human)
TagsMACExpressionMammalianMutationno mutationsAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
ELP-TEMP/pcDNA3.1(-)
Plasmid#178434PurposeGenetically encoded ratiometric fluorescent thermometerDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertELP-TEMP
TagsNo tagExpressionMammalianAvailable sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP-noPS
Plasmid#177943PurposeTransient mammalian expression of eGFP. Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserteGFP
ExpressionMammalianAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
slc1a3b:myrGFP-P2A-H2AmCherry
Plasmid#170208PurposeDrives myrGFP-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGFP labels the membrane and mCherry labels the nucleusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertslc1a3b (slc1a3b Zebrafish)
UseZebrafishTagsmyrGFP-P2A-H2AmCherryPromoterslc1a3bAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3 H2B-mIFP T2A Mpro (wt)
Plasmid#163079PurposeExpresses wild type Mpro (active) and a near-infrared fluorescent protein mIFP fused to H2BDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertWild type Mpro of SARS-CoV-2 and H2B-mIFP
ExpressionMammalianAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1_ 2xFLAG-2xSTREP_KEAP1
Plasmid#159128Purposeexpresses KEAP1 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertKEAP1 (KEAP1 Human)
Tags2X-FLAG _ 2X-STREPExpressionMammalianPromoterCMVAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gαi2-LgB91
Plasmid#134358PurposeNanoluc complementation assay. Expression of Gαi2 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi2. Addition of the HA epitope at N terminus of Gαi2.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGαi2-LgB91 (GNAI2 Human)
TagsHAExpressionBacterial and MammalianPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlpha15-RLuc8
Plasmid#140984PurposeEncodes a G alpha subunit (GNA15) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGAlpha15-RLuc8 (GNA15 Human)
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only