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30,881 results

Showing: 6301 - 6320 of 30881 results

  1. pTetON-DeAct-GS1

    Plasmid
    #89454
    Purpose
    Tet/Dox-inducible expression of EGFP-GS1 (Human Gelsolin 53-176) in mammalian cells
    Depositor
    Brad Zuchero
    Publication
    Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DeAct-GS1 (GSN Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Truncation spanning cytoplasmic Gsn amino acids 5…
    Promoter
    TRE3G
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    89454-plasmid-map-sequence-id-176821

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  2. sFlt1-i13 V5

    Plasmid
    #86044
    Purpose
    human Soluble Flt1 isoform i13 (terminates in intron 13)
    Depositor
    Christie Thomas
    Publication
    Thomas et al J Clin Endocrinol Metab. 2009 Jul;94(7):
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human soluble Flt1 isoform i13 (FLT1 Human)
    Tags
    6xHis and V5
    Expression
    Mammalian
    Mutation
    isoform i13 (terminates in intron 13)
    Promoter
    CMV
    Available since
    April 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    86044-plasmid-map-sequence-id-171579

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  5. pTRE-Tight-BI-Gl NORM-LacZA-TER-LacZB

    Plasmid
    #86194
    Purpose
    beta Globin NMD construct with and without PTC under Bi-directional TET on promoter
    Depositor
    Robert Singer
    Publication
    Trcek et al Genes Dev. 2013 Mar 1;27(5):541-51. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    beta Globin (HBB, Hbb Human, Mouse)
    Use
    Tet on
    Expression
    Mammalian
    Mutation
    39TER in beta Globin in MCS II
    Promoter
    Tet on
    Available since
    Jan. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    86194-plasmid-map-sequence-id-169938

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  6. ITSN-mcherry-sspB

    Plasmid
    #85220
    Purpose
    Role of Cdc-42 selective GEF domain from ITSN in immune cell migration
    Depositor
    Narasimhan Gautam
    Publication
    O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    ITSN1(1225-1575) (ITSN1 Human)
    mcherry
    sspB
    Tags
    NA
    Expression
    Mammalian
    Mutation
    R73Q
    Promoter
    CMV
    Available since
    Jan. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    85220-plasmid-map-sequence-id-169605

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  9. pSLQ2814 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SpdCas9

    Plasmid
    #84247
    Purpose
    Expresses direct fusion VPR-Sp dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    VPR-tagBFP-Sp dCas9
    Puro
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), HA tag, VPR, and tagBFP
    Expression
    Mammalian
    Promoter
    CAG and PGK
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    84247-plasmid-map-sequence-id-160890

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  13. pMA-SpCas9-g1

    Plasmid
    #80784
    Purpose
    BsaI-based cloning of SpCas9 gRNA guide sequence, position 1/11/21 in the array
    Depositor
    Yonglun Luo
    Publication
    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRISPR gRNA expression cassette (for SpCas9)
    Use
    CRISPR
    Tags
    na
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    80784-plasmid-map-sequence-id-162402

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  15. TetO-dCas9-D3A

    Plasmid
    #78254
    Purpose
    Expresses dead Cas9 (dCas9) fused to DNMT3A catalytic domain (CD) linked to IRES-mCherry under the control of TetOp and a minimal CMV promoter
    Depositor
    Grant Challen
    Publication
    McDonald et al Biol Open. 2016 May 11. pii: bio.019067.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DNMT3A CD aa 598-912 (DNMT3A Human)
    Use
    CRISPR
    Tags
    Flag epitope tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    78254-plasmid-map-sequence-id-182128

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  16. pcDNA3.tgoGAP1

    Plasmid
    #78737
    Purpose
    Expression of Ca2+ sensor GAP1 in the trans Golgi apparatus
    Depositor
    Teresa Alonso
    Publication
    Rodriguez-Garcia et al Proc Natl Acad Sci U S A. 2014 Feb 18;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    tgoGAP1
    Tags
    N terminal 81 amino acids of human galactosyltran…
    Expression
    Mammalian
    Mutation
    D117A/D119A/D163A in aequorin moiety
    Promoter
    CMV
    Available since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  19. pcDNA3.1 CLN3-GFP

    Plasmid
    #78110
    Purpose
    Mammalian expression of human CLN3 with C terminal GFP fusion
    Depositor
    Thomas Braulke
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CLN3 (CLN3 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  20. pDisplay sHRPa-KDEL

    Plasmid
    #73149
    Purpose
    Large fragment of split HRP targeted for expression in the lumen of the endoplasmic reticulum (ER). CMV promoter.
    Depositor
    Alice Ting
    Publication
    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sHRPa-KDEL
    Tags
    FLAG tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73149-plasmid-map-sequence-id-134530

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Showing: 6301 - 6320 of 30881 results