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30,881 results

Showing: 6621 - 6640 of 30881 results

  2. TDP-REGv2(mScarlet-FLAG)#9 in pTwist-CMV backbone

    Plasmid
    #216153
    Purpose
    Expresses mScarlet in cells with TDP-43 loss-of-function. Has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells (brighter than #7). It uses a single intron. [Code 'B11']. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Publication
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mScarlet with cryptic splice site and C-terminal FLAG tag
    Expression
    Mammalian
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216153-plasmid-map-sequence-id-437757

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  3. p101_CRISPRai_PiggyBac

    Plasmid
    #213777
    Purpose
    Expresses Tet-On inducible CRISPRai orthogonal system with VPR-dSaCas9, dSpCas9-KRAB-BFP, zeocin resistance gene, and Tet-On transactivator. PiggyBac vector.
    Depositor
    Howard Chang
    Publication
    Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    VPR-dSaCas9
    dSpCas9-KRAB-BFP
    zeocin
    Tet-On transactivator
    Use
    CRISPR
    Promoter
    EF1a (EF-1-alpha intron a) and Tet_On_TRE
    Available since
    May 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    213777-plasmid-map-sequence-id-431902

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  5. ER-B

    Plasmid
    #209870
    Purpose
    Dimerization dependent fluorescent protein B anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1
    Depositor
    Sarah Cohen
    Publication
    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ddGFP B
    Tags
    Targeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    209870-plasmid-map-sequence-id-415452

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  7. pcDNA3-endo-EKAR4

    Plasmid
    #205513
    Purpose
    Genetically encoded FRET-based biosensor for monitoring ERK kinase activity. Targeted to endosomes.
    Depositor
    Jin Zhang
    Publication
    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    endo-EKAR4
    Tags
    2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and 6xHI…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    205513-plasmid-map-sequence-id-409396

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  10. pDV06_feedback_entry_TagBFP-2A-MCS-2A-mNeonGreen-2A-MCP-ADAR2dd(E488Q)-NES

    Plasmid
    #200402
    Purpose
    Entry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon target detection
    Depositor
    James Collins
    Publication
    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Closed-loop DART VADAR sensor entry cassette
    Use
    Synthetic Biology
    Expression
    Mammalian
    Available since
    June 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    200402-plasmid-map-sequence-id-396788

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  11. dCas12Max-ABE

    Plasmid
    #195335
    Purpose
    vector for encoding a human codon-optimized dCas12Max-ABE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Publication
    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon-optimized dCas12Max-ABE
    Expression
    Mammalian
    Mutation
    N243R, E336R, D1049A
    Promoter
    CBh, CMV, hU6
    Available since
    March 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    195335-plasmid-map-sequence-id-383124

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  12. pFB2E

    Plasmid
    #195288
    Purpose
    Bicistronic HSV-TK-EGFP fusion and Puromycin resistance for positive/negative selection
    Depositor
    Scott Lowe
    Publication
    Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HSV-TK EGFP 2A Puro
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Jan. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    195288-plasmid-map-sequence-id-382892

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  13. GFP-PASS

    Plasmid
    #193970
    Purpose
    Monitoring the production of phosphatidic acid with GFP-PASS (phosphatidic acid biosensor with superior sensitivity)
    Depositor
    Guangwei Du
    Publication
    Zhang et al Mol Cell Biol. 2014 Jan;34(1):84-95. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PA biosenor
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    193970-plasmid-map-sequence-id-382205

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  16. pST_140_LVL2 cam

    Plasmid
    #179334
    Purpose
    NT-CRISPR plasmid for a single gRNA with spG Cas9 (near PAM-less Cas9 variant).
    Depositor
    Anke Becker
    Publication
    Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ptac tfoX, Ptet spG cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
    Use
    CRISPR
    Mutation
    Cas9 -> SpG Cas9 (D1135L, S1136W, G1218K, E121…
    Available since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    179334-plasmid-map-sequence-id-357079

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  18. pATZ-Myc

    Plasmid
    #177718
    Purpose
    ER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant), myc tagged
    Depositor
    Erik Böttger
    Publication
    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    alpha-1-antitrypsin (SERPINA1 Human)
    Tags
    Myc
    Expression
    Bacterial and Mammalian
    Mutation
    E342K (ATZ mutant)
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    177718-plasmid-map-sequence-id-352604

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  20. pCMV-PE2-P2A-BSD

    Plasmid
    #174819
    Purpose
    Mammalian expression of SpCas9 prime editor 2 with P2A-BSD marker
    Depositor
    David Liu
    Publication
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE2-P2A-BSD
    Tags
    P2A-BSD and SV40 bpNLS
    Expression
    Mammalian
    Mutation
    Detailed in manuscript
    Promoter
    CMV
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    174819-plasmid-map-sequence-id-343617

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Showing: 6621 - 6640 of 30881 results