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27,006 results

Showing: 6721 - 6740 of 27006 results

  1. CBE-T1.52

    Plasmid
    #193285
    Purpose
    Expression of CBE-T1.52 base editor in mammalian cells, CMV promoter
    Depositor
    Nicole Gaudelli
    Publication
    Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CBE-T1.52
    Expression
    Mammalian
    Mutation
    D10A (Cas9), TadA mutations within
    Promoter
    CMV
    Available since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193285-plasmid-map-sequence-id-379826

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  5. pCMV-eNme2-T.2-ABE8e

    Plasmid
    #185669
    Purpose
    Expresses eNme2-T.2-ABE8e in mammalian cells
    Depositor
    David Liu
    Publication
    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ecTadA(8e)-neNme2-T.2
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Nme2Cas9 D16A/E47K/R63K/V68M/A116T/T123A/D152N/E1…
    Available since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    185669-plasmid-map-sequence-id-373132

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  6. pSBbi_RP_lbMicroID2-HA

    Plasmid
    #188522
    Purpose
    Encodes the low background biotin proximity labeling enzyme lbMicroID2 for expression and stable integration into mammalian cells
    Depositor
    James Londino
    Publication
    Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    lbMicroID2
    Use
    Transposon mediated gene integration
    Tags
    HA
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available since
    Sept. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    188522-plasmid-map-sequence-id-372813

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  7. pCMV-T7-ABE8.20m-nSpCas9-NG-P2A-EGFP (KAC1164)

    Plasmid
    #185919
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ABE8.20m-nSpCas9-NG-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185919-plasmid-map-sequence-id-367356

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  11. pCMV-Spike

    Plasmid
    #173921
    Purpose
    Expresses the original SARS-Cov-2 Spike Protein under a CMV Promoter
    Depositor
    Andreas Stuermer
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-2 Spike Protein (S Human, Synthetic, SARS-COV-2)
    Tags
    no tags, native protein
    Expression
    Bacterial and Mammalian
    Promoter
    standard CMV
    Available since
    April 11, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    173921-plasmid-map-sequence-id-358849

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  13. pST_140_LVL2 cam

    Plasmid
    #179334
    Purpose
    NT-CRISPR plasmid for a single gRNA with spG Cas9 (near PAM-less Cas9 variant).
    Depositor
    Anke Becker
    Publication
    Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ptac tfoX, Ptet spG cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
    Use
    CRISPR
    Mutation
    Cas9 -> SpG Cas9 (D1135L, S1136W, G1218K, E121…
    Available since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    179334-plasmid-map-sequence-id-357079

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  14. pDB4695

    Plasmid
    #171127
    Purpose
    A plasmid containing the codon-optimized OsTIR1-F74A, which is under the control of adh1 promoter. It served as one of the F-box protein expression plasmids in AID system.
    Depositor
    Li-Lin Du
    Publication
    Zhang et al G3 (Bethesda). 2022 Jan 4;12(1):jkab393.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    OsTIR1-F74A
    Use
    Other
    Mutation
    non applicable
    Available since
    Aug. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    171127-plasmid-map-sequence-id-341099

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  15. pCMV-Csy4-PE2

    Plasmid
    #172667
    Purpose
    Enhanced prime editing system in mammalian cells. This plasmid is used by PE3 and PE3b
    Depositor
    Xiaolong Wang
    Publication
    Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Csy4-PE2
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    172667-plasmid-map-sequence-id-339511

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  16. CMV-VPR-S-L1-dCjCas9 MiniCAFE

    Plasmid
    #169910
    Purpose
    Expression of a truncated VPR-dCjCas9 fusion protein to activate gene expression.
    Depositor
    Zhili Rong
    Publication
    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MiniCAFE
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D8A, HNH-truncation (Δ495–609 aa) in CjCas9
    Promoter
    CMV
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169910-plasmid-map-sequence-id-336045

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  17. pCMV-RdLight1

    Plasmid
    #125706
    Purpose
    Expresses the red genetically encoded dopamine sensor RdLight1 in mammalian cells.
    Depositor
    Lin Tian
    Publication
    Patriarchi et al Nat Methods. 2020 Nov;17(11):1147-1155.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RdLight1
    Tags
    Flag tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    125706-plasmid-map-sequence-id-246158

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  18. pCMVd1-NLS-GAL4-iLight-VP16-T2A-mTagBFP2

    Plasmid
    #170273
    Purpose
    NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2 construct expression in mammalian cells.
    Depositor
    Vladislav Verkhusha
    Publication
    Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2
    Expression
    Mammalian
    Mutation
    See Comments
    Promoter
    CMVd1
    Available since
    June 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    170273-plasmid-map-sequence-id-334945

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  20. GFP-PIK3C2B

    Plasmid
    #161989
    Purpose
    Mammalian expression vector containing PIK3C2B tagged with GFP
    Depositor
    David Bryant, Volker Haucke
    Publication
    Marat et al Science. 2017 Jun 2;356(6341):968-972. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PIK3C2B (PIK3C2B Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    siRNA resistance to siRNA#2 with 4 silent mutatio…
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    161989-plasmid-map-sequence-id-333558

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Showing: 6721 - 6740 of 27006 results