Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

30,881 results

Showing: 6941 - 6960 of 30881 results

  2. pYPQ151

    Plasmid
    #69302
    Purpose
    Gateway entry vector with pcoCas9D10A
    Depositor
    Yiping Qi
    Publication
    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pcoCas9D10A (Plant codon-optimized)
    Use
    CRISPR
    Tags
    2XFLAG
    Mutation
    D10A mutation; NLS fusion
    Available since
    Oct. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    69302-plasmid-map-sequence-id-122140

    Enlarge

  4. PTH1R-Tango

    Plasmid
    #66489
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PTH1R (PTH1R Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  5. HCA2-Tango

    Plasmid
    #66396
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HCA2 (HCAR2 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    66396-plasmid-map-sequence-id-372748

    Enlarge

  6. Beta-2-adrenergic receptor-CFP

    Plasmid
    #55794
    Purpose
    Beta-2-adrenergic receptor tagged with ECFP on carboxyl terminus
    Depositor
    Catherine Berlot
    Publication
    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Beta-2-adrenergic receptor-ECFP (ADRB2 Aequorea victoria, Human)
    Tags
    ECFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    55794-plasmid-map-sequence-id-86731

    Enlarge

  10. pCAG-HA-rat Nrxn3beta AS4(-)

    Plasmid
    #58269
    Purpose
    Expression plasmid for HA-tagged rat Neurexin 3beta, lacking the insert at alternatively spliced segment 4 (AS4-)
    Depositor
    Peter Scheiffele
    Publication
    Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nrxn3beta AS4(-) (Nrxn3 Rat)
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    June 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58269-plasmid-map-sequence-id-107089

    Enlarge

  11. pcDNA3-hsGFP

    Plasmid
    #64912
    Purpose
    Encodes a Fluorescent Reporter for Hydrogen Sulfide in Mammalian Cells
    Depositor
    Huiwang Ai
    Publication
    Chen et al Biochemistry. 2014 Sep 23;53(37):5966-74
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hsGFP
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    Chromophore tyrosine of the protein mutated to TA…
    Promoter
    CMV
    Available since
    May 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    64912-plasmid-map-sequence-id-109661

    Enlarge

  12. pEGFP(C3)-Nup153-C (880-1475)

    Plasmid
    #64317
    Purpose
    GFP-tagged mammalian expression of C-terminal domain of Nup153
    Depositor
    Birthe Fahrenkrog
    Publication
    Duheron et al Nucleus. 2014;5(6):601-12. doi: 10.4161/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nup153 (NUP153 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    C-terminal subunit (880-1475)
    Promoter
    CMV
    Available since
    April 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  14. CyTERM-mGFP

    Plasmid
    #62237
    Purpose
    ER targeted transmembrane domain with EGFP oriented towards the cytoplasm. Used for assessing fluorescent protein oligomerization.
    Depositor
    Erik Snapp
    Publication
    Costantini et al Traffic. 2012 May;13(5):643-9. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CyTERM-EGFP
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    signal anchor of rabbit cytochrome p450 and monom…
    Promoter
    CMV
    Available since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62237-plasmid-map-sequence-id-320621

    Enlarge

  15. mEos3.2-Actin-C-18

    Plasmid
    #57443
    Purpose
    Localization: Actin, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Actin (ACTB Human)
    Tags
    mEos3.2
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 12, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by

  17. pcDNA3.1-CibN-dCas9

    Plasmid
    #60551
    Purpose
    Expresses CibN-dCas9 in mammalian cells
    Depositor
    Charles Gersbach
    Publication
    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CibN-dCas9
    Use
    CRISPR
    Tags
    FLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    inactivating Cas9 mutations D10A and H840A
    Promoter
    CMV
    Available since
    Feb. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60551-plasmid-map-sequence-id-97050

    Enlarge

  18. GANLS-DEVD-BNES

    Plasmid
    #50835
    Purpose
    Expresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 2 (BNLS).
    Depositor
    Robert Campbell
    Publication
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ddGFP A and ddRFP B
    Tags
    NES sequence LALKLAGLDIGS and triplicated NLS seq…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    50835-plasmid-map-sequence-id-76278

    Enlarge

  19. mCherry-ARP3-C-12

    Plasmid
    #54981
    Purpose
    Localization: Actin Related Protein, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ARP3 (ACTR3 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    54981-plasmid-map-sequence-id-485109

    Enlarge

  20. mCherry-FAK-5

    Plasmid
    #55044
    Purpose
    Localization: Focal adhesion kinase, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FAK
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    55044-plasmid-map-sequence-id-218555

    Enlarge

Showing: 6941 - 6960 of 30881 results