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27,006 results

Showing: 7021 - 7040 of 27006 results

  2. p55-H2B-Dendra2

    Plasmid
    #80609
    Purpose
    Encodes H2B-Dendra2-fusion protein under the CAG promoter. Linearize using Asc1 and Not1 for mouse embryo injection.
    Depositor
    Periklis Pantazis
    Publication
    Mohr et al Nat Protoc. 2016 Dec;11(12):2419-2431. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    H2B-Dendra2 (H2BC21 Human, Synthetic)
    Tags
    Dendra2
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Feb. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    80609-plasmid-map-sequence-id-159250

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  5. pSLQ2813 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-ABI-tagBFP-SpdCas9

    Plasmid
    #84242
    Purpose
    Expresses GA-inducible KRAB-Sp dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GAI-tagBFP-Sp dCas9
    GID1-KRAB
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), GAI, GID1, HA tag, IRES-Puro, and t…
    Expression
    Mammalian
    Promoter
    CAG and PGK
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    84242-plasmid-map-sequence-id-160883

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  8. pMA-SpCas9-g3

    Plasmid
    #80786
    Purpose
    BsaI-based cloning of SpCas9 gRNA guide sequence, position 3/13/23 in the array
    Depositor
    Yonglun Luo
    Publication
    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRISPR gRNA expression cassette (for SpCas9)
    Use
    CRISPR
    Tags
    na
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    80786-plasmid-map-sequence-id-162406

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  9. FLAG_hPPP1R15B_4-713_mCherry_UK1298

    Plasmid
    #80707
    Purpose
    mammalian expression N-term FLAG and C-term mCherry hPPP1R15B (CReP 4-713)
    Depositor
    David Ron
    Publication
    Chen et al Elife. 2015 Mar 16;4. doi: 10.7554/eLife
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human PPP1R15B (PPP1R15B Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    CDS 4-713 no stop codon
    Promoter
    CMV
    Available since
    Aug. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    80707-plasmid-map-sequence-id-153227

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  10. RP4-11

    Plasmid
    #79812
    Purpose
    Modified RP4 plasmid with Km resistance and sacB gene. Easily curable helper plasmid
    Depositor
    Michael Hynes
    Publication
    Quandt et al Plasmid. 2004 Jul;52(1):1-12.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Kanamycin cassette and SacB gene (sacB )
    Use
    Helper plasmid for mobilization
    Promoter
    Km
    Available since
    Aug. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  11. c3GIC9n

    Plasmid
    #62192
    Purpose
    col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9n (D10A variant) expression. Contains NsiI cloning site for U6-sgRNA cassettes
    Depositor
    Lukas Dow
    Publication
    Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. Pyogenes Cas9 (D10A)
    Use
    CRISPR
    Tags
    HA
    Mutation
    D10A substitution (Cas9n)
    Promoter
    TRE3G
    Available since
    Aug. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    62192-plasmid-map-sequence-id-156364

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  13. pPR219.05

    Plasmid
    #78554
    Purpose
    Plasmid expressing UirR, response regulator and PcsiR1-regulated GFP output
    Depositor
    Jeffrey Tabor
    Publication
    Ramakrishnan et al ACS Synth Biol. 2016 Apr 27.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    uirR
    Use
    Synthetic Biology
    Promoter
    J23115
    Available since
    June 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    78554-plasmid-map-sequence-id-408852

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  14. pdCASclos

    Plasmid
    #73640
    Purpose
    Transcriptional repression for gene Spo0A (CAC-2011) in Clostridium acetobutylicum ATCC 824
    Depositor
    Sheng Yang
    Publication
    Li et al Biotechnol J. 2016 May 23. doi: 10.1002/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas9
    sgRNA to spo0A
    Use
    E.coli-clostridium shuttle vector
    Mutation
    D10A, H840A
    Promoter
    Pj23119 and ptb
    Available since
    June 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    73640-plasmid-map-sequence-id-148604

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  15. pDisplay sHRPb-KDEL

    Plasmid
    #73150
    Purpose
    Small fragment of split HRP targeted for expression in the lumen of the endoplasmic reticulum (ER). CMV promoter.
    Depositor
    Alice Ting
    Publication
    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sHRPb-KDEL
    Tags
    V5 tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73150-plasmid-map-sequence-id-134536

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  18. pcDNA3.1-COXIV-COX8-dL5-2XG4S-mCer3

    Plasmid
    #73208
    Purpose
    Expresses dL5(E52D)-mCer3 fusion protein in mitochondria of mammalian cells. (MBIC5, dL5**, FAP)
    Depositor
    Marcel Bruchez
    Publication
    Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    COXIV-COX8-dL5-2XG4S-mCer3 (COX8A Human, Synthetic)
    Tags
    The FAP and mCerulean3 are fused with 2 copies of…
    Expression
    Mammalian
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    CMV
    Available since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    73208-plasmid-map-sequence-id-139728

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  19. pcDNA3.1-NLS-myc-dL5-2xG4S-mCer3

    Plasmid
    #73205
    Purpose
    Expresses myc-dL5(E52D)-mCer3 fusion protein in nuclei of mammalian cells. (MBIC5, dL5**, FAP)
    Depositor
    Marcel Bruchez
    Publication
    Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLS-myc-dL5-2XG4S-mCer3 (MYC Human, Synthetic)
    Tags
    The FAP and mCerulean3 are fused with 2 copies of…
    Expression
    Mammalian
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    CMV
    Available since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    73205-plasmid-map-sequence-id-139718

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  20. CD200-Cd4d3+4-COMP-blac-3xFLAG-6his

    Plasmid
    #71471
    Purpose
    A positive control receptor prey (rat CD200) EXPRESs plasmid for The Basic AVEXIS kit
    Depositor
    Gavin Wright
    Publication
    Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CD200 (Cd200 Rat)
    Tags
    6xHis tag, Cd4d3+4, beta-lactamase, and triple FL…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    71471-plasmid-map-sequence-id-128809

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Showing: 7021 - 7040 of 27006 results