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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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27,006 results

Showing: 7461 - 7480 of 27006 results

  1. pcDNA3.1.Gα13-LgB106

    Plasmid
    #134364
    Purpose
    Nanoluc complementation assay. Expression of Gα13 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 106 and 107 of Gα13. Addition of the HA epitope at N term of Gα13.
    Depositor
    Julien Hanson
    Publication
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gα13-LgB106 (GNA13 Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134364-plasmid-map-sequence-id-306388

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  2. pcDNA5/FRT/TO-GAlphai2-RLuc8

    Plasmid
    #140974
    Purpose
    Encodes a G alpha subunit (GNAI2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GAlphai2-Rluc8 (GNAI2 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    140974-plasmid-map-sequence-id-300657

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  3. pcDNA5/FRT/TO-GAlphaGustducin-RLuc8

    Plasmid
    #140979
    Purpose
    Encodes a G alpha subunit (GNAT3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GAlphaGustducin-RLuc8 (GNAT3 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140979-plasmid-map-sequence-id-300662

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  8. pEGFP-C1-SopF

    Plasmid
    #137734
    Purpose
    Expresses EGFP fused to SopF
    Depositor
    Leigh Knodler
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SopF
    Tags
    EGFP
    Expression
    Mammalian
    Available since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    137734-plasmid-map-sequence-id-271547

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  9. pCX-SponGee

    Plasmid
    #134775
    Purpose
    mammalian expression of SponGee, a buffer of cGMP that prevents downstream signaling
    Depositor
    Xavier Nicol
    Publication
    Ros et al Cell Rep. 2019 Jun 25;27(13):4003-4012.e
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SponGee
    Tags
    FLAG and mRFP
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    134775-plasmid-map-sequence-id-266288

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  12. SECURE miniABEmax-V82G (pJUL1828)

    Plasmid
    #131313
    Purpose
    CMV promoter expression plasmid for bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with V82G mutation).
    Depositor
    Keith Joung
    Publication
    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    131313-plasmid-map-sequence-id-254655

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  14. pLenti-CMV-Blast-DNTGFBR2-HA

    Plasmid
    #130888
    Purpose
    Expresses dominant negative mutant human TGFBR2
    Depositor
    Gian-Paolo Dotto
    Publication
    Bordignon et al Cell Rep. 2019 Aug 27;28(9):2358-2372.e6
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TGFBR2 (TGFBR2 Human)
    Tags
    HA-tag
    Expression
    Mammalian
    Mutation
    Dominant Negative receptor lacking cytosolic cata…
    Promoter
    CMV
    Available since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    130888-plasmid-map-sequence-id-255056

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Showing: 7461 - 7480 of 27006 results