30,881 results
-
Plasmid#78862PurposeDULIP negative control (prey) to exclude unspecific background bindingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry
UseExpression vectorTagsFirefly luciferase, V5-tagExpressionMammalianPromoterCMVAvailable sinceJuly 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRK-flag-USP19 C506S
Plasmid#78584PurposeExpression flag-USP19 C506S in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertflag-USP19 C506S (USP19 Human)
TagsflagExpressionMammalianMutationmutate 506 cysteine residue to serinePromoterCMVAvailable sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CAG sHRPa-FRB-pre mGRASP
Plasmid#73145PurposeLarge fragment of split HRP and FRB fused to the extracellular terminus of the "pre mGRASP" scaffold published by Magee, in which the extracellular domain of NRX is replaced by portions of CD4.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsHRPa-FRB-pre mGRASP
TagsFlag tagExpressionMammalianPromoterCAGAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-cofilin-S3D
Plasmid#78297PurposeMammalian expression of mouse cofilin S3D fused to EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcofilin 1 (Cfl1 Mouse)
TagsEGFPExpressionMammalianMutationS3DPromoterCAGAvailable sinceMay 17, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPB-hCMV1-hKLF2-IV
Plasmid#60433Purposetet-inducible human KLF4DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertKLF2 (KLF2 Human)
ExpressionMammalianAvailable sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag::UbvG08 P69L, L70V, I44A, deltaGG
Plasmid#74940PurposeUbiquitin variant with mutations L69P and V70L reverted to wild type (i53-DM mutant)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertUbiquitin
TagsFlagExpressionMammalianMutationUbvG08 with I44A, P69L, and L70V mutations and no…PromoterCMVAvailable sinceMay 13, 2016AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pECE M2-SNX17 WT
Plasmid#69811PurposeExpresses M2-SNX17 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSorting nexin-17 (SNX17 Human)
TagsFLAGExpressionMammalianPromoterSV40 early promoterAvailable sinceMay 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
Cag.Luc.puro
Plasmid#74409PurposeConstitutive Luciferase expression in mammalian cells along with puromycin selectionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLuciferase
ExpressionMammalianPromoterCAGAvailable sinceApril 26, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDNA3-deltaBamHI-human MIZ1
Plasmid#74167PurposeMammalian expression of human MIZ1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMIZ1 (ZBTB17 Human)
ExpressionMammalianPromoterCMVAvailable sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Rlucrarr3-Flash4
Plasmid#74132PurposeFlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 225 of Arrestin3.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertArrestin3
TagsRenilla luciferase (rLuc)ExpressionMammalianMutationInsertion of FlAsH motif (CCPGCC) following amino…Available sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Rlucrarr3-Flash3
Plasmid#74131PurposeFlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 171 of Arrestin3.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertArrestin3
TagsRenilla luciferase (rLuc)ExpressionMammalianMutationInsertion of FlAsH motif (CCPGCC) following amino…Available sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSG5-BALF5
Plasmid#72630PurposeMammalian expression vector for Epstein-Barr virus DNA polymeraseDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBALF5
ExpressionMammalianMutationG249SAvailable sinceFeb. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
PGL4-4X TE Down (GQs) Renilla
Plasmid#70039PurposePlasmid for testing 5’UTR TE Down (G-quadruplex) folding elementsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert4x TE Down (G-quadruplexes) in 5'UTR
UseLuciferaseTagsrenilla luciferaseExpressionMammalianMutation4 repeats of TE down motif (G-quadruplexes) in 5&…PromoterSV40Available sinceJan. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pY017 (pcDNA3.1-hPcCpf1)
Plasmid#69989PurposeExpresses humanized PcCpf1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthPcCpf1
UseCRISPRTagsNLS & 3xHAExpressionMammalianPromoterCMVAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pY012 (pcDNA3.1-hCMtCpf1)
Plasmid#69984PurposeExpresses humanized CMtCpf1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthCMtCpf1
UseCRISPRTagsNLS-3xHAExpressionMammalianPromoterCMVAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRJPaph-bjGFP
Plasmid#67032PurposePlasmid for Paph-bjGFP integration downstream of B. diazoefficiens (B. japonicum) scoIDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsB. diazoefficiens DNA from scoI downstream region (comprising blr1132')
bjGFP
UseConjugative bacterial vectorAvailable sinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJW1354
Plasmid#69490PurposeGly-Ser-4xGly linker-degron-TEV-3xFLAG with 40 bp nhr-25 homology armsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGSGGGG spacer-Degron-TEV protease site-3x FLAG epitope
UseCRISPR; Template to generate cassettes for -media…Available sinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
PX403 Streptococcus thermophilus LMD-9 CRISPR 3 Cas9
Plasmid#68337PurposeStreptococcus thermophilus LMD-9 CRISPR 3 Cas9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSt3Cas9
UseCRISPRTagsHA and NLSExpressionMammalianPromoterCBhAvailable sinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSNAP-CLC-EYFP
Plasmid#38011DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertClathrin, light polypeptide (LCa) (Clta Mouse)
TagsEYFP and SNAP26mExpressionBacterial and MammalianPromoterCMVAvailable sinceSept. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
RXFP1-Tango
Plasmid#66492PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRXFP1 (RXFP1 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only