30,881 results
-
Plasmid#110736PurposeEncodes Cas9 nickase and gRNA targeting CDKN2A Exon2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCDKN2A gRNA (CDKN2A Human)
UseCRISPRExpressionMammalianAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHDR-CDKN2A-Ex2-CMV-EGFP
Plasmid#110734PurposeRecombination template for replacing CDKN2A Exon2 with CMV-EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEGFP
UseOtherAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSKC11 - UTRQ
Plasmid#110490PurposeMonitor the expression of Luciferase driven by the NRAS 5'-UTR.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNRAS 5'-UTR (bp 1-254) (NRAS Human)
UseLuciferaseTagsFirefly luciferasePromoterT7Available sinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSEMS-SDHB-Halo7Tag
Plasmid#108973PurposeSelflabeling tag at succinate dehydrogenase complex subunit B (SDHB)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsuccinate dehydrogenase complex iron sulfur subunit B (SDHB Human)
TagsHalo7-tagExpressionMammalianPromoterCMVAvailable sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
tCRMod-GGSGGS5
Plasmid#111760PurposeExpresses soluble Clover-mRuby2 tension sensor module with 5-repeat GGSGGS extensible domainDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertClover-GGSGGS5-mRuby2 fusion
ExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCI syn iGluu
Plasmid#106122PurposeFast indicator for synaptic glutamate imaging (synapsin promoter)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertiGluSnFR S72T variant
TagsMyc and PDGFRExpressionMammalianMutationChanged Ser 72 to ThrPromotersynapsinAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-N2cP
Plasmid#100808Purposeexpress rabies virus CVS N2c strain phosphoprotein gene (N2cP)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertrabies virus CVS N2c strain phosphoprotein gene
ExpressionMammalianPromoterCAGAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
hCXCR4-LSSmOrange
Plasmid#110197PurposeEncodes for human CXCR4 coding sequence tagged with the LSS-mOrange FPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthCXCR4 (CXCR4 Human)
TagsLSSmOrangeExpressionMammalianPromoterCMVAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEM705 Gsz
Plasmid#109355PurposeGsX chimera with Gαz C-terminusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGsz chimera
TagsNoneExpressionMammalianPromoterCAGAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEXP(V5-APEX2-NES)
Plasmid#107596PurposeExpresses V5-APEX2-NES in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAPEX2-NES
UseFrt site to insert into hek293 t-rex genomeTagsNES and V5ExpressionMammalianPromoterCMV Dox inducibleAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPV-TetO-SpCas9-GR-iC-ERiP (CRONUS-Puro)
Plasmid#100596PurposepiggyBac vector expressing Dual-regulated Cas9 (Puro resistance)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsCRISPR Cas9 fused with human Glucocorticoid Receptor
mCherry
rtTA-M2
UsePiggybac vectorExpressionMammalianMutationCodon-optimized for human codon usagePromoterDox-inducible TetO promoter and Human EEF1A1 prom…Available sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
eMscL WT
Plasmid#107454PurposeeMscL WT is optimized for mammalian rodent neuronal expression to the plasma membrane through a neuron-specific promoter and a voltage-gated channel targeting motifDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertbacterial mechanosensitive ion channel of large conductance (mscL Escherichia Coli)
UseAdeno-associated viralTagsKir2.1 ER export signal (FCYENEV) and tdTomato fl…ExpressionMammalianPromoterhuman synapsin 1Available sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
cacnb4 (rat) in pMT2 vector
Plasmid#107426PurposeExpresses Calcium channel beta4 auxillary subunit in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcacnb4 (Cacnb4 Rat)
ExpressionMammalianPromoterAd MLPAvailable sinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MX2_pCSdest
Plasmid#53942DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMX2 (MX2 Human)
ExpressionMammalianPromoterSP6 and CMVAvailable sinceMarch 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
USP25_pCSdest
Plasmid#53817DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertUSP25 (USP25 Human)
ExpressionMammalianPromoterSP6 and CMVAvailable sinceMarch 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
GW1-mito-roGFP1-mApple
Plasmid#107218PurposeMammalian expression plasmid to target the roGFP1-mApple sensor to the mitochondrial matrix.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertroGFP1-mApple
Tags4xCox8ExpressionMammalianPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
BPK1169: CAG-DmrC-NLS-3xFLAG-P65
Plasmid#104564PurposeMammalian expression vector for DmrC fused to p65 activation domainDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDmrC-p65
Tags3x FLAG and NLSExpressionMammalianPromoterCAGAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GST-BHMT-I/G
Plasmid#104442PurposeAutophagy cargo assayDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBHMT (BHMT Human)
TagsGFP-myc GFP is from Aequorea victoria and GSTExpressionMammalianMutationN43A in GST (in a potential KFERQ-like sequence).…PromoterCMVAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
Plasmid#101103PurposeEncodes dCas9(C)-VP64 with SAM components fused to VEGFR1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertVEGFR1-dCas9(C)
UseCRISPR and Synthetic BiologyTagsHAExpressionMammalianAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVEGFR2_TEV(N)_NES_TCS(Q'L)_HA-dCas9(N)_P2A_Puro-WPRE
Plasmid#101104PurposeEncodes dCas9(N) fused to VEGFR2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertVEGFR2-dCas9(N)
UseCRISPR and Synthetic BiologyTagsHAExpressionMammalianAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only