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30,881 results

Showing: 10821 - 10840 of 30881 results

  3. pCMV-T7-ABE8e-SpRY-HiFi-P2A-EGFP (LM458)

    Plasmid
    #197508
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpRY-HiFi(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pCMV-T7-BPNLS-ABE8e-nSpRY-HiFi-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpRY-HiFi(D10A/A61R/…
    Promoter
    CMV and T7
    Available since
    March 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    197508-plasmid-map-sequence-id-387292

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  4. Td-CBEmax

    Plasmid
    #196600
    Purpose
    Mammalian expression of Td-CBEmax
    Depositor
    Dali Li
    Publication
    Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    bpNLS-TadA-8e (N46L/E27R)-32aa linker-nSpCas9-bpNLS-P2A-UGI-bpNLS
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    TadA-8e (N46L/E27R); S. pyogenes Cas9 (D10A)
    Available since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    196600-plasmid-map-sequence-id-386793

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  7. plk1 T210D

    Plasmid
    #132964
    Purpose
    To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage
    Depositor
    Michael Yaffe
    Publication
    Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    polo-like kinase 1 (PLK1 Human)
    Expression
    Mammalian
    Mutation
    T210D
    Available since
    Feb. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    132964-plasmid-map-sequence-id-265932

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  8. opto-Cdc42

    Plasmid
    #174510
    Purpose
    Expresses opto-Cdc42 (mCherry-GGGSx2-Cdc42-GGGSx2-BcLOV4-GGGS-3xFLAG) in a pcDNA3.1 backbone.
    Depositor
    Brian Chow
    Publication
    Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    opto-Cdc42-mCherry
    Tags
    3xFLAG and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    174510-plasmid-map-sequence-id-357203

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  10. Fucci(CA) hCdt1-iRFP hGeminin-iRFP

    Plasmid
    #190182
    Purpose
    Fluorescent reporter vector for visualization of G0 and other cell cycle phases.
    Depositor
    Toshiro Sato
    Publication
    Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    iRFP
    hCdt1(1/100)Cy(-) (Cdt1 Synthetic)
    iRFP
    hGeminin(1/110)
    Expression
    Bacterial and Mammalian
    Promoter
    CMV
    Available since
    Feb. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    190182-plasmid-map-sequence-id-378850

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  11. pGL4_10xUAS-MLPmin-luc2

    Plasmid
    #194383
    Purpose
    Reporter plasmid for split TEV assays; firefly luciferase reporter gene driven by 10x clustered UAS binding sites linked to a minimal MLP promoter
    Depositor
    Michael Wehr
    Publication
    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    10xUAS-luc2
    Expression
    Mammalian
    Promoter
    MLP minimal
    Available since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    194383-plasmid-map-sequence-id-381605

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  12. pFB2G

    Plasmid
    #195290
    Purpose
    Bicistronic HSV-TK and mTagBFP resistance for positive/negative selection
    Depositor
    Scott Lowe
    Publication
    Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HSV-TK 2A mTagBFP
    Expression
    Mammalian
    Promoter
    PGK
    Available since
    Jan. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    195290-plasmid-map-sequence-id-382902

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  19. His-FLAG-Tev_ACTB (beta-actin)

    Plasmid
    #188453
    Purpose
    Express in mammalian cells human beta-actin (ACTB) with N-terminal 6xHis tag, FLAG tag, and TEV protease site.
    Depositor
    Roberto Dominguez
    Publication
    Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ACTB (ACTB Human)
    Tags
    6xHis-FLAG
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    CMV
    Available since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    188453-plasmid-map-sequence-id-373094

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  20. pVC-FUS-WT

    Plasmid
    #175066
    Purpose
    expresses mVenus and mCherry tagged to FUS wildtype in mammalian cells
    Depositor
    Arnold Boersma
    Publication
    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FUS glycine rich protein (FUS Human)
    Tags
    mVenus + mCherry
    Expression
    Mammalian
    Mutation
    mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…
    Promoter
    CMV Promoter
    Available since
    Oct. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175066-plasmid-map-sequence-id-372335

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Showing: 10821 - 10840 of 30881 results