30,881 results
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Plasmid#201489PurposeNanoBit detection of V5-tagged proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNbV5-HiBiT
TagsHiBitExpressionMammalianPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE TurboID-HA donor (all-in-one) ORF1
Plasmid#200384PurposeTagging endogenously expressed proteins with TurboID-HA, C-terminalDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTurboID (ref: PMID: 30125270, Addgene plasmid # 107169)
ExpressionMammalianMutationNAAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
PAmCherry1-C1-P4Mx2
Plasmid#202681PurposePhotoactivatable lipid biosensorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPAmCherry1: L. pneumophila SidM(546-647):SidM(546-647) (sidM Legionella pneumophila)
ExpressionMammalianAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FLT3-V5/HIS
Plasmid#201985Purposeexpression of human FLT3 receptor tyrosine kinase in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFLT3 receptor tyrosine kinase (FLT3 Human)
TagsV5, 6xHisExpressionMammalianPromoterCMVAvailable sinceJune 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
mtsCOXVIIIx2-B-gTEMP/pcDNA3
Plasmid#199806PurposeFluorescent temperature indicator B-gTEMP fused with mitochondrial targeting sequenceDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertB-gTEMP
TagsmtsCOXVIIIx2ExpressionMammalianAvailable sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-DDR1-V5/HIS
Plasmid#201100Purposeexpression of human DDR1 receptor tyrosine kinase in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman DDR1 receptor tyrosine kinase, full length, wildtype (DDR1 Human)
TagsV5/HisExpressionMammalianPromoterCMVAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
PGK 4xMito-mEmerald
Plasmid#200430PurposeMammalian expression of fluorescently-tagged marker for mitochondriaDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert4x cytochrome c oxidase subunit VIII mitochondrial targeting sequence
TagsmEmeraldExpressionMammalianMutation4x mito-targeting sequencePromoterPGKAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AW01_pU.CAG.eSpCas9(1.1).rBGpA
Plasmid#199255PurposeExpression construct encoding the high specificity eSpCas9(1.1) nucleaseDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHigh specificity eSpCas9(1.1) nuclease
UseCRISPRTags3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationK848A K1003A R1060APromoterCAG promoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-APPP1-mGFP
Plasmid#196705PurposeExpresses mCherry-APP-TagGFP2, with substitution at position 612 that prevents alpha-secretase activityDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAmyloid Precursor Protein 695 isoform (APP Human)
TagsTagGFP2 and mCherryExpressionMammalianMutationLys to Val substitution at position 612Available sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTET2-RlucCP-3MS2
Plasmid#198354PurposePart of Renilla reporter system, encodes Renilla luciferase with MS2 stem loops in the 3'UTRDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRenilla Luciferase
UseLuciferaseExpressionMammalianPromoterpTET2Available sinceApril 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
Membrane EGFP
Plasmid#198056PurposePlasma membrane localizing EGFP using Human LCK proto-oncogene sequenceDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLCK membrane EGFP (LCK Synthetic, A. victoria, Human)
UseSea urchin, mammal, zebrafishTagsEGFPMutationTandem repeat of N-terminal LCK membrane targetin…Promotersp6Available sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPB-TetON-mEGFP-HMGB1-WT
Plasmid#194561PurposeDox-inducible Mammalian Expression of mEGFP-HMGB1 WT IDR Fusion ProteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHMGB1 (HMGB1 Human)
TagsmEGFPExpressionMammalianAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-myrlysin/BORCS5
Plasmid#194332PurposeExpresses myrlysin in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBORCS5 (BORCS5 Human)
TagsGFPExpressionMammalianPromoterCMVAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBtet rtTA G72V SE Δsplice GP Luc
Plasmid#194328PurposeTetracycline inducible expression of Luciferase enzyme in mammalian cells with inclusion of rtTA G72V mutation, splice site and high sensitivity mutations for increased promotor tightnessDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLuciferase
UseLuciferaseExpressionMammalianMutationTet-responsive promotor related mutations: G72V i…PromoterTight tet-responsive promotorAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
iBE4max YE1 NG CLYBL
Plasmid#174570Purposedoxycycline inducible BE4max YE1 NG expression for human CLYBL locus knock-inDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBE3
ExpressionMammalianPromotertet ONAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
mGFP-hNET
Plasmid#193364Purposeexpression of human norpinephrine transporter tagged with monomeric EGFP at the N-terminus in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman Norepinephrine Transporter (SLC6A2 Human)
Tagsmonomeric EGFP (mEGFP)ExpressionMammalianPromoterCMVAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX459_gRNA-AAVS1_hspCas9
Plasmid#193309PurposeCas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)
UseCRISPRExpressionMammalianMutationnot applicableAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpCas9 TadCBEa
Plasmid#193782PurposeExpress TadCBEa (with SpCas9) in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTadA-CDa-SpCas9D10A
ExpressionMammalianAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PhoDer
Plasmid#188473PurposeExpresses the optogenetic human Gasdermin D (PhoDer) in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPhoDer (GSDMD Synthetic, Human)
ExpressionMammalianPromoterCMV IEAvailable sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAK_DR30_CasRx_mCherry
Plasmid#134844PurposeAll in one overexpression of CasRx and guideRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRfxCas13d
UseCRISPRTagsT2A mCherryExpressionMammalianPromoterCAGAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only