29,692 results

1. pTara:500

   Plasmid

   #60717

   Purpose

   Expresses T7 RNAP from the arabinose inducible promoter PBAD with a RBS strength of 500 from the Salis RBS calculator. Also includes a constitutive araC ORF

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Insert

   T7 RNAP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD

   Available since

   Nov. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGST2-Rabex5 (9-73)

   Plasmid

   #17630

   Depositor

   James Hurley

   Article

   Lee et al Nat Struct Mol Biol. 2006 Mar . 13(3):26

   Insert

   Rabex5 (9-73) (RABGEF1 )
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Ubiquitin-binding domain: residues 9-73

   Promoter

   Available since

   March 28, 2008

   Availability

   Academic Institutions and Nonprofits only

3. pORTMAGE-4

   Plasmid

   #72679

   Purpose

   Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Cam resistance marker.

   Depositor

   Csaba Pál

   Article

   Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p

   Insert

   mutL E32K
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   E32K mutation conferring dominant mutator phenoty…

   Promoter

   Available since

   Feb. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCoofy4

   Plasmid

   #43986

   Depositor

   Sabine Suppmann

   Article

   Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

   Type

   Empty backbone

   Use

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. tdTomato-anti-AlfaTag nanobody

   Plasmid

   #171819

   Purpose

   Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

   Depositor

   Ikuo Wada

   Article

   Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

   Insert

   tdTomato-anti-AlfaTag nanobody
   

   Use

   Tags

   6xHis and SEP tag (6xD)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAL-pck_T

   Plasmid

   #74068

   Purpose

   pAL374 plasmid carrying the pck (T) sgRNA targeting, the template strand of pck, SpecR

   Depositor

   Timothy Lu

   Article

   Cleto et al ACS Synth Biol. 2016 Feb 16.

   Insert

   sgRNA pck (T)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   ptac

   Available since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pAWP78-PVCpnf_pvc13-E01DARPin

   Plasmid

   #198287

   Purpose

   Produces PVCs retargeted with anti-EGFR DARPin E01

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   PVCpnf_pvc13-E01DARPin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET30-R5-GFP

   Plasmid

   #17838

   Depositor

   Jay Keasling

   Article

   Shaikh et al Anal Chem. 2008 Feb 1. 80(3):886-90.

   Insert

   pET30-GFP
   

   Use

   Synthetic Biology

   Tags

   His, R5, and S-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET15bpATnp

   Plasmid

   #121137

   Purpose

   This vector encode a His6-tagged proteinA-Tnp(hyperactive form) fusion protein.

   Depositor

   Keji Zhao

   Article

   Carter et al Nat Commun. 2019 Aug 20;10(1):3747. doi:

   Insert

   Protein A-Tnp
   

   Use

   Tags

   his

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28-iLOVf

    Plasmid

    #63723

    Purpose

    E coli expression plasmid to produce Thrombin cleavable His tagged iLOV fusion proteins

    Depositor

    Brian Smith

    Type

    Empty backbone

    Use

    Tags

    TEE, iLOV, 6xHis, thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mNeptune-pBAD

    Plasmid

    #54714

    Purpose

    Localization: Protein Expression Vector , Excitation: 600, Emission: 650

    Depositor

    Roger Tsien, Robert Campbell, Nathan Shaner, Michael Davidson

    Article

    Lin et al Chem Biol. 2009 Nov 25;16(11):1169-79. d

    Type

    Empty backbone

    Use

    Tags

    6xHis and mNeptune

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pF

    Plasmid

    #42519

    Depositor

    Terry Magnuson

    Article

    Jang et al PLoS One. 2013;8(2):e57075. doi: 10.1371

    Type

    Empty backbone

    Use

    Unspecified; Cloning

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7, T3

    Available since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAT219

    Plasmid

    #134887

    Purpose

    Bacterial expression

    Depositor

    Thomas Howard

    Article

    Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

    Insert

    mRFP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKSJ340

    Plasmid

    #27125

    Depositor

    Alan Finkelstein

    Article

    Jakes et al Mol Microbiol. 2010 Feb;75(3):567-78. do

    Insert

    Colicin Ia + Ia immunity protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pGEX4T1-human beta-catenin full length

    Plasmid

    #132609

    Purpose

    expresses human beta-catenin full length in E. coli

    Depositor

    Wenqing Xu

    Article

    Xing et al Structure. 2008 Mar;16(3):478-87. doi: 1

    Insert

    beta-catenin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHH0103_ESRRB_Hormone-recep

    Plasmid

    #109909

    Purpose

    Protein expression and purification of ESRRB_Hormone-recep

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    ESRRB_Hormone-recep (ESRRB Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. UbcH5c WT pET28a

    Plasmid

    #12643

    Depositor

    Rachel Klevit

    Article

    Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

    Insert

    UbcH5c - Ubiquitin conjugating enzyme (UBE2D3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 31, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBAD_GFP11_T7LysH17A

    Plasmid

    #59591

    Purpose

    Expression vector for solubility screening of proteins or protein fragments using a split-GFP assay.

    Depositor

    Richard Kingston

    Article

    Bulloch et al Anal Biochem. 2014 Jul 10. pii: S0003-26

    Type

    Empty backbone

    Use

    Tags

    6xHis-tag and GFP11

    Expression

    Bacterial

    Mutation

    Promoter

    pARA

    Available since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. SPLICSFAST-ER-PO-P2A

    Plasmid

    #214894

    Purpose

    Expresses equimolar Peroxisome- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-PO Contact sites

    Depositor

    Tito Calì

    Insert

    SPLICSFAST-ER-PO-P2A
    

    Use

    Tags

    none

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET32a-hEPR

    Plasmid

    #199233

    Purpose

    Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

    Depositor

    Nilson Zanchin

    Article

    Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

    Insert

    Proepiregulin preproprotein (EREG Human)
    

    Use

    Tags

    TrxA-6xHis-S-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge