33,902 results
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Plasmid#55653Purposeexpression of mouse HRASDepositorAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only
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pACλCI-β-flap (831-1057)
Plasmid#53733PurposeExpression of λCI fused via three alanines to E. coli RNAP beta subunit, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.DepositorPublicationInsertλCI-β-flap fusion
ExpressionBacterialPromoterlac UV5Available sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBRα-β flap (831-1057)
Plasmid#53734PurposeExpression of alpha NTD fused via three alanines to E. coli RNAP subunit beta, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.DepositorPublicationInsertalpha NTD-Beta flap fusion
ExpressionBacterialPromoterlpp/lacUV5 (tandem promoter)Available sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a-Ec.coaA
Plasmid#50386PurposeHeterologous expression of E. coli CoA biosynthetic enzyme, PanK (pantothenate kinase), induced with IPTG. Contain 6xHis-tag for nickel affinity purification.DepositorInsertPanK
Tags6XHis-tagExpressionBacterialPromoterT7Available sinceMarch 13, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTD103aiiA
Plasmid#48884PurposeNegative feedback plasmid from oscillatorDepositorInsertaiiA and LuxR
UseSynthetic BiologyTagsssrA (TSAANDENYALAA)ExpressionBacterialPromoterpLuxAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TRBP
Plasmid#50351Purposebacterial expression of TRBPDepositorAvailable sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pQF-lacZ
Plasmid#48094PurposeCumate-inducible expression of lacZDepositorInsertE. coli lacZ
ExpressionBacterialPromoterPQ5Available sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRF-PhlF
Plasmid#49367DepositorInsertPhlF repressor
UseSynthetic BiologyExpressionBacterialAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET C-terminal TEV His6 cloning vector with BioBrick polycistronic restriction sites (9Bc)
Plasmid#48285DepositorTypeEmpty backboneTagsHis6ExpressionBacterialAvailable sinceNov. 5, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTDpelB-NTwinStrep
Plasmid#45940Purposeuse to create N-terminal PelB-Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal PelB-Twin-Strep-tag and C-terminal 6x His-tag for periplasmic translocation via PelB signal sequenceDepositorTypeEmpty backboneTags6xHis, PelB, and Twin-StrepExpressionBacterialPromoterTrc (lactose/IPTG inducible)Available sinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTD-NStrepHis
Plasmid#45936Purposeuse to create N-terminal Strep-tagII fusion proteins or dual tagged fusion with N-terminal Strep-tagII and C-terminal 6x His-tagDepositorTypeEmpty backboneTags6xHis and Strep IIExpressionBacterialPromoterTrc (lactose/IPTG inducible)Available sinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGPD2
Plasmid#43972DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastPromoterGPDAvailable sinceApril 9, 2013AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
CREBBP
Plasmid#38977PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRE107
Plasmid#43829DepositorPublicationTypeEmpty backboneUseBacterial allelic exchange vector with sacb1ExpressionBacterialAvailable sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pQTEV-ASF1A
Plasmid#31591DepositorInsertASF1 anti-silencing function 1 homolog A (S. cerevisiae) (ASF1A Human)
TagsHis and TEVExpressionBacterialAvailable sinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p15TV-L
Plasmid#26093PurposeSGC Empty backbone for bacterial expression under T7 promoter. Uses infusion based cloning method.DepositorTypeEmpty backboneTags6xHis and TEV cleavage siteExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available sinceSept. 6, 2012AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
HA-mDyn2 pcDNA3
Plasmid#36264DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDynamin 2 (Dnm2 Mouse)
TagsHAExpressionBacterial and MammalianPromoterCMVAvailable sinceApril 20, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDEST14-SpyCatcher EQ
Plasmid#35045DepositorInsertSpyCatcher EQ
TagsHis6ExpressionBacterialMutationDeletion of C-terminal till I113, and point mutat…Available sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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pDuet TDP-43 M337V
Plasmid#27464DepositorInsertTDP-43 (TARDBP Human)
UseCoexpressionExpressionBacterialMutationMethionine 337 mutated to Valine (M337V)Available sinceFeb. 10, 2011AvailabilityAcademic Institutions and Nonprofits only