32,120 results
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Plasmid#198322PurposeCumate-inducible gene expression in diverse gram negative bacteria. Features also include mobilisation via oriT, a gigantic multiple cloning site, and blue/white screening via amilCP chromoproteinDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromotercumate-inducibleAvailable SinceApril 12, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits
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pYC2085
Plasmid#158721PurposeCRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using hygromycin as a selection marker.DepositorInsertSth1 sgRNA scaffold
UseCRISPRExpressionBacterialAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
BbZIP-cALFA
Plasmid#160498PurposeFor the protein expression of BbZIP with ALFA tag at C-terminus in E. coliDepositorInsertBbZIP
TagsHRV3C protease cleavage site-ALFA tag-8histidineExpressionBacterialAvailable SinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQE30-His-IF2
Plasmid#124124PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETM6
Plasmid#49795PurposeePathBrick vector for pathway engineering in E. coli.DepositorTypeEmpty backboneTagsS-tagExpressionBacterialPromoterT7Available SinceJan. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SpdCas9-LBiT_Strep-Tag-II
Plasmid#198751PurposeExpresses S. Pyogenes dCas9 fused to LargeBiT fragment of split NanoLuc luciferaseDepositorInsertSpdCas9 - LargeBiT
TagsStrep-tag IIExpressionBacterialMutationD10A, H840A (nuclease inactivating mutations in &…PromoterT7Available SinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFC331
Plasmid#87843PurposeAMA1 plasmid with Aspergillus optimized Cas9 and argB selection markerDepositorInsertsCas9
argB
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialPromoterAspergillus nidulans tef1 promoter and native pro…Available SinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCW-LIC
Plasmid#26098PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneTagsThree consecutive Taq promoters, 2 activeExpressionBacterialAvailable SinceAug. 20, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFDH
Plasmid#131706PurposeExpress constitutively an NAD-dependent formate dehydrogenase from Pseudomonas sp. 101 in E. coliDepositorInsertformate dehydrogenase
Tagshis-tagExpressionBacterialAvailable SinceNov. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJCC_076 AsCas12a R1226A
Plasmid#171669PurposeFor bacterial expression of AsCas12a R1226A (RuvC-inhibiting mutation) with an N-terminal His-MBP tagDepositorInsertAsCas12a R1226A
Tags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged arginine 1226 to alanineAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFV2-HA
Plasmid#187082PurposeExpression of ATP synthase, with all Cys changed to Ala, and subunit a-HA tagged, subunit beta-His tagged)DepositorInsertatpBEFHAGDC
Tags6-His beta subunit (atpD) and HA tag subunit a (a…ExpressionBacterialMutationAll Cys codons changed to Ala.Available SinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
NSUN2
Plasmid#188059PurposeBacterial expression plasmid for human NSUN2; for activity testingDepositorAvailable SinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQE60-EFG-His
Plasmid#124126PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
hCD12
Plasmid#225541PurposeExpresses human histone deacetylase 6 (HDAC6) catalytic domain 1 and 2 (CD12), also known as hCD12, in Escherichia coli cells.DepositorInsertHuman Histone Deacetylase 6 Catalytic Domain 1 and 2 (HDAC6 Human)
TagsHis6-MBP tag with cleavable TEV siteExpressionBacterialPromoterT7Available SinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETx-SR6v15
Plasmid#176212PurposeBacterial expression of the SR6v15 nanobody targeting the receptor binding domain of SARS-CoV-2 spike proteinDepositorInsertSR6v15
Tags6XHisExpressionBacterialPromoterT7Available SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
LB1353 (Bcl-2 promoter, no IgH enh)
Plasmid#15393DepositorInsertB-cell lymphoma/leukemia-2 (BCL2 Human)
UseLuciferaseTagsLuciferaseExpressionBacterial and MammalianMutationBcl-2 with full promoter and no IgH enhancers MHS…Available SinceAug. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
5WCI (MYST1)
Plasmid#101269PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceOct. 2, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJUMP27ts-1A(sfGFP)
Plasmid#126975PurposeLevel 1 vector. sfGFP reporter. Thermosensitive origin pSC101 (low copy number)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialMutationRepA A56V thermosensitive mutationAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1237
Plasmid#119262Purposefor cloning new sgRNAsDepositorInsertdcas9
ExpressionBacterialMutationD10A and H840AAvailable SinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRISPathBrick
Plasmid#65006PurposeE. coli vector for expression of S. pyogenes dCas9, tracrRNA, and nontargeting CRISPR array with BsaI site for inserting user-defined spacer-repeat bricksDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionBacterialPromoterConstitutive native promotersAvailable SinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
BBa_J72214-BBa_J72090
Plasmid#40782DepositorInsertVioABCDE
UseSynthetic Biology; PhagemidExpressionBacterialAvailable SinceOct. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-mNeongreen-Etanercept
Plasmid#182421PurposeBacterial expression of Vesicle Nucleating peptide-mNeongreen-Etanercept fusionDepositorInsertVNp-mNeongreen-Etanercept (TNFRSF1B Human)
TagsVesicle Nucleating peptide (VNp) & mNeongreenExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pST39
Plasmid#64009Purposeoriginal polycistronic cloning vector for plasmid suite (generation I)DepositorTypeEmpty backboneExpressionBacterialPromoterT7Available SinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDIV151
Plasmid#226180PurposeExpresses Cas9 in a backbone compatible with K. phaffii.DepositorInsertCas9
UseCRISPRTagsSV40ExpressionBacterial and YeastPromoterGAP1Available SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB405-TIC21-sGFP
Plasmid#213865PurposeBinary vector for the expression of sGFP flanked with an N-terminal chloroplas inner envelope membrane transit peptide in plants .DepositorInsertchloroplast inner envelope membrane transit peptide (TIC21 Mustard Weed)
ExpressionBacterial and PlantAvailable SinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQE30-His-AsnRS
Plasmid#124105PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBFC0993
Plasmid#231993PurposeCRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with negative control (RFP-targeting) spacerDepositorInsertsdRfxCas13d
crRNA with negative control (RFP-targeting) spacer
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterJ23119 and pTetAvailable SinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDB-His-MBP-mGSDMD
Plasmid#123365PurposeExpression of full-length mouse GSDMD in E. coliDepositorAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
BK Dunlop
Plasmid#25466DepositorInserthuman papovavirus BK Dunlop
ExpressionBacterialAvailable SinceSept. 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
PrecA-CFP--PrecA-YFP
Plasmid#228097PurposerecA promoter driving expression of CFP reporter and recA promoter driving expression of YFP reporterDepositorInsertCFP & YFP
ExpressionBacterialPromoterrecA & recAAvailable SinceMay 28, 2025AvailabilityAcademic Institutions and Nonprofits only