32,069 results
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Plasmid#98550Purposeempty BB3 for insertion and overexpression of 1 gene in P. pastorisDepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only
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HK3
Plasmid#25303PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHK3:R480-R922 (HK3 Human)
TagsHisExpressionBacterialMutationContains amino acids R480-R922Available SinceAug. 16, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQLinkHD-SYNZIP6
Plasmid#80662PurposeEncodes the synthetic coiled coil peptide SYNZIP6DepositorInsertSYNZIP6
Tags6xHisExpressionBacterialAvailable SinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQLinkHD-SYNZIP5
Plasmid#80661PurposeEncodes the synthetic coiled coil peptide SYNZIP5DepositorInsertSYNZIP5
Tags6xHisExpressionBacterialAvailable SinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_BCL2L1_BH4+Bcl-2
Plasmid#109993PurposeProtein expression and purification of BCL2L1_BH4+Bcl-2DepositorInsertBCL2L1_BH4+Bcl-2 (BCL2L1 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
TOPO CFP
Plasmid#68489PurposeTOPO construct expressing CFP for generation of GMAP-compatible geneDepositorInsertCyan Fluorescent Protein
UseGmapExpressionBacterialPromoterPlacAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTetQCas-BsaI
Plasmid#170629PurposeExpression of CRISPR-associated transposasesDepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI)
ExpressionBacterialAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCJ209
Plasmid#162682PurposeLidless variant of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) replacing Gly251:Thr472 of MHETase with the 7-residue loop (Trp185:Phe191, PETase numbering) of PETase , codon optimized for expression in E. coli K12, with C-terminal His tag.DepositorInsertMHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) with the lid removed
TagsHisExpressionBacterialMutationReplacement of G251-T472 with W185-F191 from PETa…PromoterT7/lacAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMTTH+eGFP-bromo5
Plasmid#157804PurposeExpression of synthetic protein composed of eGFP and 5 repeats of BRD4 bromodomain #1DepositorInserteGFP-bromo5
TagsHis6 and MBPExpressionBacterialPromoterpTACAvailable SinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R364
Plasmid#138234PurposeExpresses the split mCherry reporter interrupted by the Cth-Ter artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only